2015
DOI: 10.1136/gutjnl-2014-308491
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Identification and genetic manipulation of human and mouse oesophageal stem cells

Abstract: We developed clonogenic and organotypic culture systems for the quantitative analyses of human and mouse oesophageal stem/progenitor cells and identified novel cell surface marker combinations that enrich for these cells. Using this system, we demonstrate that elimination of p63 inhibits self-renewal of human oesophageal stem/progenitor cells. We anticipate that these esophagosphere culture systems will facilitate studies of oesophageal stem cell biology and may prove useful for ex vivo expansion of human oeso… Show more

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Cited by 27 publications
(38 citation statements)
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“…A layer of proliferating basal cells attached to the basement membrane produces several layers of differentiated cells, which are shed into the lumen and replaced by cells derived from the basal cells. While basal cells have stem cell properties, it remains unclear whether a unique self-renewing and quiescent stem cell population exists in the esophagus (121)(122)(123)(124)(125). The stomach, in turn, is lined by an epithelium composed of crypt-like structures called gastric units that produce gastric acid (in the corpus) or the hormone gastrin (in the Antrum) (126).…”
Section: Mammalian Intestinal Stem Cellsmentioning
confidence: 99%
“…A layer of proliferating basal cells attached to the basement membrane produces several layers of differentiated cells, which are shed into the lumen and replaced by cells derived from the basal cells. While basal cells have stem cell properties, it remains unclear whether a unique self-renewing and quiescent stem cell population exists in the esophagus (121)(122)(123)(124)(125). The stomach, in turn, is lined by an epithelium composed of crypt-like structures called gastric units that produce gastric acid (in the corpus) or the hormone gastrin (in the Antrum) (126).…”
Section: Mammalian Intestinal Stem Cellsmentioning
confidence: 99%
“…Despite uncertainty on the exact nature of the human esophageal progenitor population, progenitor properties of the basal layer cells are supported by their ability to form colonies that express markers of undifferentiated epithelium in culture and differentiate into esophageal spheroids in defined medium. 49,50 Most superficial cells in the suprabasal zone of the epithelium are nondividing and considered terminally differentiated cells.…”
Section: Properties Of the Esophageal Epitheliummentioning
confidence: 99%
“…Work in esophageal squamous cell carcinoma (ESCC) cell lines suggests that p63 may be required for epithelial proliferation (85). Although the in vivo role of p63 in adult esophageal epithelium has not been addressed, genetic deletion of p63 in organotypic culture derived from adult esophageal epithelium indeed inhibited their self-renewal (30).…”
Section: Regulators Of Esophageal Epithelial Proliferation and Differmentioning
confidence: 99%
“…Additionally, methods have now been established for both mouse and human 3D organotypic esophageal epithelial cultures (17,30,69). Culturing of such esophagospheres will provide an easy platform to advance our insights into the regulation of the esophageal stem/progenitor cell populations and epithelial homeostasis.…”
Section: Esophageal Stem Cellsmentioning
confidence: 99%
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