Identification and genetic monitoring of mouse inbred strains using biochemical polymorphisms

Groen, Arnoud

doi:10.1258/002367777780936567

Cited by 27 publications

(15 citation statements)

References 22 publications

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“…In the past, most research workers have taken the authenticity of such strains on trust, but sometimes this trust is misplaced (Festing, 1974;Krog, 1976;Groen, 1977). Moreover, eyen if strains do not become genetically contaminated, errors in labelling when animals are transported could result in research workers inadvertently using the wrong animals.…”

Section: Discussionmentioning

confidence: 99%

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Polyvalent strain-specific alloantisera as tools for routine genetic quality control of inbred and congenic strains of rats and mice

Festing

Totman

1980

Lab Anim

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SummaryStrain-specific polyvalent alloantisera may be obtained by injecting lymphocytes pooled from several different strains into an inbred recipient. 6 sera ofthis type were produced in rats and 23 in mice. A dye-exclusion microcytotoxic test was used to evaluate the strain specificity of such sera. A total of 663 out of 713 (93.0%) of the tests conformed with expectation, but there were 48 (6· 7%) false negative results in which the test failed to detect non-authentic animals. There were also 2 (0·3%) false positive results, in which authentic animals were shown as non-authentic. These were attributed to technical errors. Most false negative results occurred when serum and test cell suspensions matched at the major histocompatibility complex.It was concluded that the use of strain-specific polyvalent immune sera, coupled with a simple immunological test such as the microcytotoxic test, offers a sensitive and quick new method for routine genetic quality control.

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Section: Discussionmentioning

confidence: 99%

“…Biochemical marker genes (Groen, 1977;Hoffman, 1978) are useful but their study is technically complicated and time-consuming. Skin grafting is sensitive for discovering whether a strain is inbred, but is clumsy as a means of strain identification: it also takes far too long (12-100 days) to get an answer.…”

Section: Discussionmentioning

confidence: 99%

Polyvalent strain-specific alloantisera as tools for routine genetic quality control of inbred and congenic strains of rats and mice

Festing

Totman

1980

Lab Anim

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“…Although the * Corresponding author. origin and genetic similarities of many American strains have been traced in detail (Potter & Klein, 1979;Staats, 1979;Roderick, Staats & Womack, 1981), fewer studies have been published for European strains, though Groen (1977) and Hilkens et al (1981) have published information on some of them. Nor have these strains been compared with those of American origin.…”

Section: Introductionmentioning

confidence: 99%

Esterase alleles of inbred mouse strains maintained in The Netherlands

Hilgers

Deimling²,

Zutphen

et al. 1988

Genet. Res.

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Fifty-seven mouse strains were examined for genetic variation at 21 esterase loci. Three new alleles were found: Es-6 d in strain A/WySna, Es-ll e in FTC/CpbU and Es-18 c in two WLL/BrA sublines. At most loci there was a single allele found in over 80 % of strains, with one or two rare alleles. However, the Es-1, 3, 10, 13, 25 and 27 loci were much more polymorphic. Although several loci were linked on chomosomes 3, 8 and 9, linkage disequilibrium was only found between Es-5 and Es-11 (chromosome 8) and Es-26 and Es-27 (chromosome 3). There was also significant disequilibrium between Es-1 and 3, Es-1 and 10, and Es-3 and 10, which are on different chromosomes, suggesting that the 57 strains are not a random sample of inbred mouse strains. Fifty-four strains were closely related, with the Es-7", -17", -18", -23 C set of alleles, which are typical of Mus musculus domesticus. The three exceptional strains were MOL3 (Mus musculus molossinus), WLL/BrA (English-Norwegian origin) and TA2 (Chinese origin). There were 10 groups of strains which were identical at all loci. Sublines of the same strain were usually identical. Sometimes more distantly related strains, such as CBA/Bi, C3H/He, SM and DBA/Li, were identical, and in a few cases strains with no known common ancestry such as C58 and MAS were identical. Attempts to discriminate between a subset of 22 American and 15 European strains were unsuccessful, suggesting that the European strains add only in a quantitative manner to the gene pool of 'laboratory mice', whereas wild-derived strains such as MOL3 are genetically quite distinct from other laboratory mice.

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“…The genetic monitoring of rodent colonies demands the use of immunological and biochemical techniques (Groen, 1977;Hoffman, 1978;Festing & Totman, 1980). Many of these are costly, technically complicated and time consuming.…”

mentioning

confidence: 99%

Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens

Fernández¹,

Weeks²

1986

Lab Anim

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Identification and genetic monitoring of mouse inbred strains using biochemical polymorphisms

Cited by 27 publications

References 22 publications

Polyvalent strain-specific alloantisera as tools for routine genetic quality control of inbred and congenic strains of rats and mice

Polyvalent strain-specific alloantisera as tools for routine genetic quality control of inbred and congenic strains of rats and mice

Esterase alleles of inbred mouse strains maintained in The Netherlands

Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens

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