Identification and Growth Characterization of a Novel 2,4-D (Dichlorophenoxyacetic Acid) Degrading Bacterium Maribacter Sp Amsu Isolated From Aquaculture Effluent

Abstract

Sankaralingam et al.: Identification and growth characterization of a novel 2,4-D (dichlorophenoxyacetic acid)However the bacterial growth was sustained and a significant death was observed with culture media containing 800mg/100ml 2,4-D and above. At regular time intervals the optical density was measured at 600nm. Growth of the isolate was determined by viable cell enumeration immediately after inoculation. The growth of Maribacter sp in medium containing 50mg/100ml to 200mg/100ml 2,4-D was not varied much during 24 to 48h, and the total viable count of 121 and 144 × 10 -4 CFU.ml -1 were observed, respectively. Media optimization results indicated that lactose (carbon) and ammonium chloride (nitrogen) added medium at pH 7 and 35 °C found to be suitable for the better growth of Maribacter sp. But lead added medium inhibited the growth of Maribacter sp. Degradation of 2,4D in culture supernatant was determined by using HPLC and the result obtained was discussed.