Identification and histopathological and pathogenicity analysis of Aeromonas salmonicida salmonicida from goldfish (Carassius auratus) in North China

Jin, Shibo; Fu, Songzhe; Li, Ruijun; Dang, Huifeng; Gao, Dongxu; Ye, Shigen; Jiang, Zhiqiang

doi:10.1016/j.aaf.2019.04.004

Cited by 11 publications

(8 citation statements)

References 24 publications

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“…(Preena et al 2019) thereby interrupting the sustainable fish culture. Mass mortality of goldfish population infected with Aeromonas hydrophila (Dharmaratnam et al 2018), A. salmonicida (Jin et al 2020), Proteus hauseri (Kumar et al 2015), Mycobacterium marinum, (Hodgkinson et al 2012), Flavobacterium columnare (Verma et al 2015) etc. was noticed in various fish farms.…”

Section: Introductionmentioning

confidence: 99%

A peek into mass mortality caused by antimicrobial resistant Edwardsiella tarda in goldfish, Carassius auratus in Kerala

2022

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Edwardsiella tarda is one of the serious threats affecting the worldwide aquaculture. In the present study, four isolates were recovered from diseased goldfish, showing hemorrhages, reported with 60% mass mortality in an ornamental fish farm, Ernakulam, Kerala. Based on the phenotypic and genotypic analysis, the bacteria were identified as Edwardsiella tarda , Citrobacter freundii, Acinetobacter junii and Comammonas testosteronii . Experimental challenge studies using healthy goldfish revealed that among the four isolates, E. tarda alone leads to 100% mortality of experimental fish within 175 degree days and the pathogen could be successfully re-isolated from the moribund fish. The LD50 value of E. tarda was calculated as 9.9 × 10 5 CFU/fish. The histopathology of the infected tissues of goldfish had shown the typical features of E .tarda infection. The pathogen was found positive for the virulence genes viz., hly , etfA , etfD and eseD as detected using PCR. Thus E. tarda was confirmed as the real causative agent of the disease outbreak. Multiple antimicrobial resistance (AMR) exhibited by the pathogen towards 19 tested antibiotics with the MAR index of 0.46 highlighted the exposure of antibiotics to the fish in the farm. The existence of antibiotic resistant genes within the plasmid as revealed through plasmid curing studies pointed out the possibility of rapid dissemination of AMR in aquaculture. Hence proper surveillance and appropriate diagnostic methods need to be implemented at regular intervals to mitigate the menace.

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Section: Introductionmentioning

confidence: 99%

A peek into mass mortality caused by antimicrobial resistant Edwardsiella tarda in goldfish, Carassius auratus in Kerala

2022

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“…Then, the anaesthetized sea cucumber was dissected, and the wounded part of the body wall was removed from the whole body and fixed with Bouin's solution for 24 hr. The fixed samples were used to prepare tissue sections according to standard methods (Jin et al., 2020).…”

Section: Methodsmentioning

confidence: 99%

Wound healing at the body wall of the sea cucumber Apostichopus japonicus by Chinese herbal treatment

et al. 2020

Aquac. Res.

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“…For staining, sectioned material was dewaxed in xylene, rehydrated through a graded ethanol series (0-100%) and stained with hematoxylin and eosin. Permanent preparations were obtained by dehydrating (through an ethanol series 50-100%), and cleaned in xylene; neutral resin was used to seal the tissue sections, which were then examined under a Leica DM4000 microscope (Jin et al 2020).…”

Section: Histopathological Analysismentioning

confidence: 99%

Aeromonas hydrophila as a Causative Agent of Fester-Needle Tail Disease in Guppies (Poecilia reticulata)

Huang¹

2021

IJAB

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Nowadays, increases in the scale of guppy (Poecilia reticulata) breeding have been accompanied by increases in the frequency of disease, resulting in economic losses. One such disease, the fester-needle tail disease was notorious. In the current study, an epidemiological investigation was carried out for 1 year, and a total of 138 needle tail cases with obvious symptoms of rot were collected from 5 guppy farms in Anshan and Liaoyang cities in Liaoning Province, China. Microscopic observations excluded fungal and parasitic infections as causes of the disease. Transmission electron microscopy did not detect any viral inclusions in the diseased site. There was a high density of nucleiin the cells, with cells becoming longer and narrower, and containing an increasing number of vesicles. Intotal, 232 bacterial strains were isolated from fish with needle tail disease. Results of 16S ribosomal DNA sequencing, and physiological and biochemical identification showed that Aeromonasspp. accounted for 35.3% of all the isolates, with Aeromonas hydrophila being the most isolated strain; in total 37strains were isolated, accounting for 45.1% of the Aeromonas spp. isolates. A bacterial challenge experiment with A. hydrophila revealed it to be a possible causative agent of fester-needle tail disease. Histopathological observations in the controlled infection showed that epithelial cells in the caudal fin became more compacted, with all cells showing different degrees of karyotheca dissolution. These results provide a reference for further research and scientific control of needle tail disease in this economically important species.© 2021 Friends Science Publishers

show abstract

Identification and histopathological and pathogenicity analysis of Aeromonas salmonicida salmonicida from goldfish (Carassius auratus) in North China

Cited by 11 publications

References 24 publications

A peek into mass mortality caused by antimicrobial resistant Edwardsiella tarda in goldfish, Carassius auratus in Kerala

A peek into mass mortality caused by antimicrobial resistant Edwardsiella tarda in goldfish, Carassius auratus in Kerala

Wound healing at the body wall of the sea cucumber Apostichopus japonicus by Chinese herbal treatment

Aeromonas hydrophila as a Causative Agent of Fester-Needle Tail Disease in Guppies (Poecilia reticulata)

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