Identification and isolation of a new x‐type HMW glutenin subunit 1Dx1.6t gene from Aegilops tauschii

An, Xianghai; Li, X. H.; Xiong, Xunhao; Yan, Yueming; Zhang, Y. Z.; Gao, Liyan; Wang, A. L.; Wang, K.; Zeller, F. J.; Hsam, S. L. K.

doi:10.1111/j.1439-0523.2008.01543.x

Cited by 13 publications

(5 citation statements)

References 21 publications

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“…To date, only limited numbers of x‐type HMW glutenin genes have been isolated from Ae. tauschii accessions (Lu et al 2005; Wan et al 2005; Zhang et al 2008b; An et al 2009 ). In the current, two novel x‐type HMW glutenins 1Dx3 t and 1Dx4 t were cloneded and characterized from Ae.…”

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confidence: 99%

Molecular characterization of HMW-GS 1Dx3t and 1Dx4t genes from Aegilops tauschii and their potential value for wheat quality improvement

Wang

Pan

et al. 2012

Hereditas

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Two x-type high molecular weight glutenin subunits (HMW-GS) in Aegilops tauschii, 1Dx3(t) and 1Dx4(t) were identified by SDS-PAGE and MALDI-TOF-MS. Their complete coding sequences were isolated by AS-PCR. 1Dx3(t) and 1Dx4(t) genes consist of 2535 bp and 2508 bp and encode 845 and 836 amino acid residues, respectively. The deduced molecular masses of 1Dx3(t) and 1Dx4(t) gene products are 87655.26 Da and 86664.24 Da, respectively, well corresponding to the molecular masses measured by MALDI-TOF-MS. A total of 18 SNPs were identified between 1Dx3(t) and 1Dx4(t). Comparing with 1Dx5 subunit, 1Dx3(t) had a six amino acid insertion at 146-151 while the 1Dx4(t) had a nine amino acid deletion when compared with 1Dx3(t) subunit. The authenticity of the cloned 1Dx3(t) and 1Dx4(t) genes were confirmed by successful expression of their ORFs in E. coli. Comparison and phylogenetic tree based on the amino acid and nucleotide sequences confirmed that 1Dx3(t) was most closely related to 1Dx5 subunit that is widely accepted as a superior subunit for bread-making property. The secondary structure prediction demonstrated that 1Dx3(t) subunit has significantly high α-helix and β-strand contents, suggesting it might have positive effects on dough quality.

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confidence: 99%

Molecular characterization of HMW-GS 1Dx3t and 1Dx4t genes from Aegilops tauschii and their potential value for wheat quality improvement

Wang

Pan

et al. 2012

Hereditas

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“…tauschii accessions found to be superior for various components of heat tolerance. Recent studies indicate good prospects for extending the range of traits that may be introgressed from Aegilops taushii (An et al. 2009, Takumi et al.…”

Section: Resultsmentioning

confidence: 99%

A direct hybridization approach to gene transfer from Aegilops tauschii Coss. to Triticum aestivum L

et al. 2010

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Aegilops tauschii Coss., the D-genome donor of bread wheat, represents a rich source of resistance and productivity traits for wheat improvement. In this study, a direct hybridization approach using Ae. tauschii as the female in crosses with Triticum aestivum resulted in about 50 times more F 1 plants than the reciprocal cross. In a set of five Ae. tauschii · T. aestivum crosses, an average of 35% of the pollinated florets resulted in embryo formation. Following embryo rescue on artificial medium, an average of 6.8 F 1 plants could be obtained for every 100 florets pollinated. Colchicine treatment of the F 1 plants greatly enhanced backcross seed formation (14.9 backcross seeds compared to an average of 0.47 seeds per 100 florets for untreated tillers). The efficacy of this gene transfer system was demonstrated by improved cell membrane stability and chlorophyll retention in BC 1 F 4 lines derived from the crosses of three heat-tolerant accessions of Ae. tauschii with bread wheat cultivar ÔPBW 550Õ.

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“…In the present study, the substitution from cysteine to arginine in the y-type subunit could be responsible for the lower dough quality by decreasing the number and affecting the pattern of disulphide cross-links in the gluten in polymers, which have been verified by site-directed mutagenesis technology. Previous studies have suggested that the number and distribution of cysteine residues were two of the key factors influencing wheat dough quality [18,34,48,49]. Although exploiting the new HMW-GSs genes from wild species was an effective way to broaden the genetic diversity of wheat [8,21,31,35,50,51], the gene engineering could be utilized to improve the dough quality by modifying the number or the structure of cysteine residues in HMW subunits [11,35].…”

Section: Discussionmentioning

confidence: 99%

“…Several favorable genes for the storage protein have been identified in Ae. tauschii [18][19][20][21][22][23][24]. However, few of them were introgressed into wheat and applied in wheat breeding.…”

Section: Introductionmentioning

confidence: 99%

Identification and Introgression of a Novel HMW-GS Gene from Aegilops tauschii

Fan²,

et al. 2022

Agronomy

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High molecular weight glutenin subunits (HMW-GSs) play a major role in determining the dough quality of wheat. As the D genome donor of hexaploid wheat, Aegilops tauschii is an important genetic resource for wheat quality breeding. In the present study, a novel HMW-GSs from Ae. tauschii was identified and designated as Glu-Dt1. Multiple sequence alignment indicated that one cysteine was mutated into arginine in the y-type subunit. Site-directed mutagenesis technology was applied to verify the function of gene Glu-Dt1. Three introgression lines (ILs), B9, B25, and B35 with the Glu-D1 loci substituted by Glu-Dt1 were detected from the BC3F5 population derived from hexaploid wheat cultivar Jimai22 and Ae. tauschii Y215 through the direct hybridization approach. The dough quality and agronomic performance analysis were performed, which provide valuable resources for wheat genetic studies and breeding for distinctive end-use quality.

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Identification and isolation of a new x‐type HMW glutenin subunit 1Dx1.6^t gene from Aegilops tauschii

Cited by 13 publications

References 21 publications

Molecular characterization of HMW-GS 1Dx3t and 1Dx4t genes from Aegilops tauschii and their potential value for wheat quality improvement

Molecular characterization of HMW-GS 1Dx3t and 1Dx4t genes from Aegilops tauschii and their potential value for wheat quality improvement

A direct hybridization approach to gene transfer from Aegilops tauschii Coss. to Triticum aestivum L

Identification and Introgression of a Novel HMW-GS Gene from Aegilops tauschii

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