Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System

Buczek, Magdalena Elżbieta; Reeder, Stephen; Regad, Tarik

doi:10.1007/978-1-4939-7401-6_13

Cited by 8 publications

(13 citation statements)

References 12 publications

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“…In addition to the isolation of PCSC populations based on marker expression, other strategies exploiting PCSC-specific gene reporters are being developed for real-time analysis of PCSC behavior at a single-cell level in vitro and in animal models. These systems are based on the stable expression of a reporter gene (e.g., fluorescent protein) driven by the promoters of PCSC-or lineage-specific genes such as NANOG, PSA, SOX2, and OCT4 [13][14][15][16][17][18] (Table 1). For example, Qin et al [3] took advantage of the fact that PSA represents one of the most lineage-specific differentiation markers in human prostate epithelia and addressed the important question of whether the phenotypically differentiated (i.e., PSA +/hi ) and undifferentiated (PSA −/lo ) PCa cell populations might be functionally distinct.…”

Section: Current Methods For Pcsc Enrichment and Analysismentioning

confidence: 99%

Concise Review: Prostate Cancer Stem Cells: Current Understanding

et al. 2018

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Prostate cancer (PCa) is heterogeneous, harboring phenotypically diverse cancer cell types. PCa cell heterogeneity is caused by genomic instability that leads to the clonal competition and evolution of the cancer genome and by epigenetic mechanisms that result in subclonal cellular differentiation. The process of tumor cell differentiation is initiated from a population of prostate cancer stem cells (PCSCs) that possess many phenotypic and functional properties of normal stem cells. Since the initial reports on PCSCs in 2005, there has been much effort to elucidate their biological properties, including unique metabolic characteristics. In this Review, we discuss the current methods for PCSC enrichment and analysis, the hallmarks of PCSC metabolism, and the role of PCSCs in tumor progression. Stem Cells 2018;36:1457-1474.

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Section: Current Methods For Pcsc Enrichment and Analysismentioning

confidence: 99%

Concise Review: Prostate Cancer Stem Cells: Current Understanding

et al. 2018

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“…It is known that normal stem cells and CSCs share core stemness signaling pathways such as Notch, Hedgehog, WNT/β-catenin and JAK/STAT, that have pivotal roles in maintaining stem cell properties and regulating their transcriptional program (Chen K. et al, 2013). SOX2, OCT4, KLF4, and NANOG are some of the key TFs known to promote stemness by upregulating genes involved in selfrenewal and pluripotency, while suppressing genes involved in differentiation (Young, 2011;Tang et al, 2015;Buczek et al, 2018). Key stem cell TFs like SOX2, OCT4, and NANOG have been proven to be overexpressed in CSCs.…”

Section: Transcription Factors As Potential Csc Markersmentioning

confidence: 99%

The Relevance of Transcription Factors in Gastric and Colorectal Cancer Stem Cells Identification and Eradication

Pádua

Figueira

Ribeiro

et al. 2020

Front. Cell Dev. Biol.

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Gastric and colorectal cancers have a high incidence and mortality worldwide. The presence of cancer stem cells (CSCs) within the tumor mass has been indicated as the main reason for tumor relapse, metastasis and therapy resistance, leading to poor overall survival. Thus, the elimination of CSCs became a crucial goal for cancer treatment. The identification of these cells has been performed by using cellsurface markers, a reliable approach, however it lacks specificity and usually differs among tumor type and in some cases even within the same type. In theory, the ideal CSC markers are those that are required to maintain their stemness features. The knowledge that CSCs exhibit characteristics comparable to normal stem cells that could be associated with the expression of similar transcription factors (TFs) including SOX2, OCT4, NANOG, KLF4 and c-Myc, and signaling pathways such as the Wnt/βcatenin, Hedgehog (Hh), Notch and PI3K/AKT/mTOR directed the attention to the use of these similarities to identify and target CSCs in different tumor types. Several studies have demonstrated that the abnormal expression of some TFs and the dysregulation of signaling pathways are associated with tumorigenesis and CSC phenotype. The disclosure of common and appropriate biomarkers for CSCs will provide an incredible tool for cancer prognosis and treatment. Therefore, this review aims to gather the new insights in gastric and colorectal CSC identification specially by using TFs as biomarkers and divulge promising drugs that have been found and tested for targeting these cells.

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“…Cancer stem cells (CSCs) are a small number of cells with the capacity for self-renewal, infinite proliferation, and multi-directional differentiation in tumor tissue [3]. Their anti-apoptotic, tumorigenic, and migratory ability differs significantly from those of highly differentiated cancer cells [3]. CSCs are considered to be the source of swelling of tumor cells with different differentiation degrees, and thus the origin of infinite proliferation and metastasis of tumors.…”

Section: Introductionmentioning

confidence: 99%

RNA Binding Protein RNPC1 Suppresses the Stemness of Human Endometrial Cancer Cells via Stabilizing MST1/2 mRNA

Wang

Zhong

et al. 2020

Med Sci Monit

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Departmental sources Background: RNA binding protein RNPC1 has a tumor-suppressive role in various tumors, nevertheless, the role of RNPC1 in human endometrial cancer (EC) are never been reported. Material/Methods: Western blot, quantitative polymerase chain reaction and sphere forming analysis were performed to evaluate the stem-like traits of cells and RNPC1-induced effects on EC cell stemness. RNA immunoprecipitation (RIP) was constructed to investigate the underlying mechanisms. Results: The spheres formed by EC cells, named EC spheres, exhibited a remarkably higher stemness than the parental cells, which is characterized as the increase of sphere forming ability, ALDH1 activity, stemness marker expression and migration ability. Notably, RNPC1 expression was decreased in poorly differentiated EC cells than that in EC cells with moderately differentiated. Additionally, RNPC1 expression was significantly decreased in EC spheres and RNPC1 overexpression attenuated the stemness of EC spheres. Moreover, RNPC1 overexpression decreased the migration ability of EC spheres. Mechanistic studies showed that RNPC1 overexpression activated the Hippo pathway through directly binding to MST1/2. Inhibition of MST1/2 rescued RNPC1-mediated effects on EC sphere stemness. Conclusions: Therefore, our results indicate a novel RNPC1/MST1/2 signaling responsible for EC cell stemness.

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Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System

Cited by 8 publications

References 12 publications

Concise Review: Prostate Cancer Stem Cells: Current Understanding

Concise Review: Prostate Cancer Stem Cells: Current Understanding

The Relevance of Transcription Factors in Gastric and Colorectal Cancer Stem Cells Identification and Eradication

RNA Binding Protein RNPC1 Suppresses the Stemness of Human Endometrial Cancer Cells via Stabilizing MST1/2 mRNA

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