Identification and Kinetic Characteristics of an Indigenous Diesel-degrading Gordonia alkanivorans Strain

Young, C. C.; Lin, Ta Chen; Yeh, Mao Song; Shen, Fo Ting; Chang, Jo Shu

doi:10.1007/s11274-005-5742-7

Cited by 47 publications

(27 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, Márquez-Rocha et al (2001) attained nearly 90 % diesel consumption after 13 days of bioremediation. In general, the diesel biodegradation by the consortia was faster than that of other microbes reported in the literature (Vieira et al 2007;Young et al 2005). These results prove the good feasibility of using polluted sites as source of mixed consortia for hydrocarbon degradation, as also has been found in similar previous works (Nikakhtari et al 2009;Vieira et al 2007).…”

Section: Resultssupporting

confidence: 86%

“…Similar K s values were reported by Young et al (2005), who reported a K s value of 3.196 g of TPH/L. Table 4 also displays the correlation coefficients of the model and the significance of the correlations.…”

Section: Parameter Estimationsupporting

confidence: 81%

“…However, this hypothesis cannot be verified, because, all of the consortia displayed similar values. Except for the assays conducted at 35°C, l max ranged between 0.17 and 0.34 h -1 , which is slightly higher than those obtained in previous studies (Whang et al 2008;Young et al 2005). …”

Section: Parameter Estimationcontrasting

confidence: 67%

See 2 more Smart Citations

Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils

Moliterni

Jiménez-Tusset

Rayo

et al. 2012

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Three microbial consortia were isolated from three polluted soils located at an oil refinery and acclimated to grow on diesel fuel as the sole carbon source. Batch experiments were then conducted with the three consortia to study the kinetics of diesel biodegradation. The effects of temperature (25, 30 and 35°C) and diesel concentration (0.5, 1 and 3 %) on the biodegradation of diesel were analysed. Several species were identified in the acclimated microbial consortia, and some of them appeared in more than one consortium. Thermal inhibition was observed at 35°C. In the rest of experiments, over 80 % of the substrate was degraded after 40 h of treatment. These results proved the good feasibility of using the polluted sites as sources of mixed consortia for hydrocarbon degradation. However, diesel degradation efficiencies and rates were very similar, suggesting that the acclimation process produced mixed consortia with very similar characteristics; in this context, origin of the soil sample was not a decisive factor. A simple Monod-type kinetic model was used to simulate the biodegradation process, and accurate results were obtained. The l max values were between 0.17 and 0.34 h -1 . The results of this study revealed that the consortia can function at high concentrations of hydrocarbons without any sign of growth inhibition, which is important for the design of bioreactors for wastewater treatment with high concentrations of fuel.

show abstract

Section: Resultssupporting

confidence: 86%

Section: Parameter Estimationsupporting

confidence: 81%

See 1 more Smart Citation

Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils

Moliterni

Jiménez-Tusset

Rayo

et al. 2012

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Genetic variation of Gordonia population in different wastewater samples as reveled by 16S rRNA gene analysis Among Actinobacteria, the genus Gordonia exhibits greater metabolic and genetic versatility [2,43]. The role of Gordonia spp.…”

Section: Discussionmentioning

confidence: 99%

Rapid quantification and analysis of genetic diversity among Gordonia populations in foaming activated sludge plants

Marrengane

Kumar

Pillay

et al. 2011

J. Basic Microbiol.

View full text Add to dashboard Cite

Activated sludge plants, sporadically suffers malfunction due to the proliferation of filamentous bacteria mainly Gordonia and Microthrix species. Nested Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (nested PCR-RFLP) in combination with quantitative realtime PCR (q PCR) was applied to study the distribution of Gordonia in foaming samples. Samples of mixed liquor were collected from three full-scale activated sludge plants that were experiencing filamentous biological foaming. Partial sequencing of 16S rRNA genes revealed the dominance of Gordonia amarae (60 -80%), Gordonia terrae (10%), Gordonia polyisoprenivorans (30 -40%) and an unidentified Gordonia species (20 -50%). Restriction enzyme analysis of the amplicons exhibited 87.14 to 99.6% similarity at interspecies level. The q PCR results showed that there was an average of 15.6% Gordonia 16S rRNA copies with respect to the total bacterial 16S rRNA gene in foaming sludge samples with the highest being 23.51% and lowest being 10.28%. The presence of Gordonia spp. in the foaming samples was observed throughout the year but was lower during winter and its presence was significantly higher in foaming samples, compared to Microthrix parvicella (r = 0.007, P < 5%). This approach could help to quantify and confirm the existence of genetically diverse indigenous Gordonia spp. in foaming samples and can be used as an indicator of forthcoming foaming incidents. Bulking and foaming bacteria are one of the major issues in Wastewater Treatment Works (WWTW) with varying wastewater characteristics and operating conditions [3,5,18,25,27,29,33]. Formation of thick stable foam on the surfaces of clarifier could lead to a significant reduction in the quality of effluent affecting the overall efficiency of the plant. Studies worldwide established the presence of filamentous bacteria viz., Thiothrix spp., G. amarae, Candidatus Microthrix parvi- [4,10,20,29,31,35,36]. The most frequent filamentous bacteria causing sludge foaming are Candidatus M. parvicella and G. amare [24]. However, numerous studies have also confirmed that the incident of biological foaming is highly correlated with the profusion of Mycolata including Gordonia amarae [24,26] which also includes four more genera viz., Mycobacterium, Nocardia, Rhodococcus and Tsukamurella [27]. Members of this genus Gordonia are aerobic, catalase-positive, gram-positive to gram-variable, slightly acid-fast, nonmotile and nocardioform actinomycetes. These microorganisms are able to rapidly assimilate substrates when available and then store them intra-cellularly as carbon and energy stores. These energy reserves can It is now evident that some operational parameters experienced by the activated sludge including low food to microorganism ratio, low dissolved oxygen concentration, sludge age and environmental conditions like temperature and pH are primarily responsible to create a selective environment for the proliferation of certain foam forming filamentous microorganisms [19,26,41]. Proliferation of certain f...

show abstract

“…The abilities of certain bacteria to utilize diesel oil as growth substrate have been extensively studied using a variety of microorganisms [5]- [8].…”

Section: Introductionmentioning

confidence: 99%

Growth Kinetics Study of a Bacterial Consortium Producing Biosurfactants, Constructed with Six Strains Isolated from an Oily Sludge

Sadouk-Hachaïchi¹,

Tazerouti²,

Hacène³

2014

ABB

View full text Add to dashboard Cite

How to cite this paper: Sadouk-Hachaïchi, Z., et al. (2014) AbstractWe investigated the ability of a bacterial community constructed with six strains isolated from an oily sludge, to utilize diesel oil at high concentrations. The consortium was able to grow at concentrations up to 84 g diesel oil/L and had produced biosurfactants during its active growth phase; these compounds, in their crude form, reduced the surface tension of distilled water from 72 mN/m to 31 mN/m, with a corresponding Critical Micelle Concentration value γ CMC = 81 mg/L. The plot of specific growth rates obtained from the growth curves versus initial concentrations was found to fit adequately the experimental data by the Andrews inhibitory model, which resulted in the following kinetic constants: μmax = 0.535d −1 ± 0.063, KS = 18.68 g/L ± 3.59 and KI = 29.02 g/L ± 4.96, reflecting the slow biodegradation rate. At 25.2 g diesel oil/L, close to the optimal concentration S * = 23.28 g/L ± 4.23, the consortium metabolized diesel oil faster than each strain did individually, suggesting that the process was stimulated by a synergistic interaction between the members of the consortium.

show abstract

Identification and Kinetic Characteristics of an Indigenous Diesel-degrading Gordonia alkanivorans Strain

Cited by 47 publications

References 21 publications

Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils

Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils

Rapid quantification and analysis of genetic diversity among Gordonia populations in foaming activated sludge plants

Growth Kinetics Study of a Bacterial Consortium Producing Biosurfactants, Constructed with Six Strains Isolated from an Oily Sludge

Contact Info

Product

Resources

About