Identification and localization of a schistosome-associated fucosyllactose determinant expressed by Fasciola hepatica

Abdul-Salam, Fatma; Mansour, Mohamed H.

doi:10.1016/s0147-9571(99)00063-6

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…Evidências experimentais sugerem que os açucares da superfície das células estão envolvidos no processo de reconhecimento entre parasita e hospedeiro, e esta mudança na ordem dos carboidratos superficiais induz modificações estruturais e funcionais nas células do hospedeiro 5 .…”

Section: Discussionunclassified

“…Estudos moleculares e imunohistoquímicos têm investigado o desenvolvimento do ovo de esquistossoma e o papel das suas secreções na iniciação, desenvolvimento e ativação da resposta imune do organismo do hospedeiro 12 . Entretanto, pouco ainda se sabe sobre o papel dos carboidratos expressos durante o desenvolvimento de parasitoses como a esquistossomose 5 .…”

Section: Discussionunclassified

“…O tegumento dos ovos de Schistosoma mansoni tem uma estrutura complexa ancorada em um citoesqueleto externo rico em carboidratos com características extremamente dinâmicas permitindo diversas interações entre o parasita e o hospedeiro 5,6 .…”

Section: Introductionunclassified

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Estudo comparativo da expressão de carboidratos no sistema ovogranuloma hepático na esquistossomose humana e experimental

Guimarães,

Melo-Junior,

Silva

et al. 2008

Rev Inst Adolfo Lutz

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Em virtude das lesões granulomatosas em animais e humanos aparentemente demonstrarem as mesmas alterações histológicas, poucas dados existem sobre as alterações patobioquímicas relacionadas aos carboidratos expressos pelos tecidos parasitados pelo S. mansoni. Neste trabalho, os resultados indicam que todas as lectinas testadas evidenciaram padrões de marcação diferenciados no tegumento do ovo do parasita e no granuloma periovular. A lectina WGA (Wheat germ agglutinin) apresentou uma intensa marcação do sistema ovo-granuloma (SOG) na esquistossomose experimental, enquanto que nas amostras teciduais humanas a WGA, LTA (Lotus tetragonolobus agglutinin) e PNA (Peanut agglutinin) marcaram apenas o ovo de S. mansoni. A lectina UEA-I (Ulex europaeus agglutinin) marcou de forma incipiente e inespecífica o SOG; por outro lado, a LTA marcou preferencialmente os anéis de fibrose do granuloma hepático em humanos. Houve intensa marcação da WGA no SOG e no ovo de S. mansoni, enquanto que a PNA marcou apenas o ovo do parasita, o qual indica a presença de resíduos de n-acetil-glucosamina e galactose, respectivamente. As lectinas WGA, PNA e Con A (Concanavalin agglutinin) falharam na distinção de tipos celulares encontrados no granuloma tanto experimental como humano. Conclui-se que a análise histoquímica com o uso de lectinas é uma ferramenta útil na investigação de alterações bioquímicas específicas que caracterizam a esquistossomose humana e experimental.

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Section: Discussionunclassified

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Estudo comparativo da expressão de carboidratos no sistema ovogranuloma hepático na esquistossomose humana e experimental

Guimarães,

Melo-Junior,

Silva

et al. 2008

Rev Inst Adolfo Lutz

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“…Evidently, fucose moieties constitute a common structural denominator that seems to be implicated in various key positions among developmentally regulated trematode oligosaccharide sequences. These included, the series of fucosylated units linked a1,3 and a1,4 to GIcNAc in egg glycosphingolipids (Van der Kleij et al 2002;Wuhrer et al 2002), the larval-associated tri-saccharide determinant, stage-specific embryonic antigen 1 (SSEA-1) (Robijn et al 2005) as well as tri-and tetra-antennary complex-type glycans, with polyfucosylated polylactosamine chains, containing the Lewis X blood group antigen (Huang et al 2001;Nyame et al 2002;Wuhrer et al 2006) or the blood group H-related fucoyllactose epitope expressed in multiple copies among larval and adult trematodes (Mansour 1996;Abdul-Salam and Mansour 2000). Although factors governing the success or failure of larval parasitic trematode development within the molluscan intermediate host are poorly understood (Loker et al 2004), this dynamic host-parasite interplay involves the interaction between the outer glycocalyx of the parasite and the cellular and humoral arms of the defense system of the host, which in turn may be the primary targets of parasite-mediated interference Loker and Bayne 2001).…”

Section: Introductionmentioning

confidence: 99%

Characterization of fucose-binding lectins in rock- and mud-dwelling snails inhabiting Kuwait Bay

Mansour

Abdul-Salam

2009

Immunobiology

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The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

Abdel-Rahman¹,

Mohamed

Abdel‐Rahman

et al. 2014

J Parasit Dis

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In the present study, the carbohydrate structures associated with Fasciola gigantica adult worm were identified by indirect hemagglutination inhibition test. Glucose was found to be the main monosaccharide associated with the fluke. According to indirect hemagglutination inhibition results, purification of glycoprotein fractions from worm crude extract was carried out by affinity chromatography immobilized glucose agarose gel and Con-A lectin columns. The isolated glycoprotein fractions, FI and FII, were characterized by SDS-PAGE which revealed one band in FI of 26 kDa and another one band of 19.5 kDa in FII compared with 12 bands associated with whole worm extract. Both fractions were also characterized by isoelectric focusing technique which proved that both bands were acidic in nature with pIs 6.4 and 6.5 respectively. The comparative diagnostic evaluation of the two isolated glycoprotein fractions and crude extract of experimental fasciolosis in rabbits by ELISA revealed that FII was more potent in the diagnosis during prepatent (first week post infection) and patent periods (10 weeks post infection) than FI and crude extract. Moreover, infected rabbit sera at ten weeks post infection identified both bands; 26 and 19.5 kDa in western blot analysis confirming its immunodiagnostic activities which was proved previously by ELISA. FII proved potency in diagnosis of fasciolosis in 200 buffalo serum samples of different ages and sexes using ELISA which recorded 95 % positive and 5 % negative samples. Moreover, the detailed structural analyses of the most potent fraction, F11, using mass spectrum was made and elucidated chemical structure; O-glycan [Ser-(Arg-Ser-Arg-Ser-GlucNAc) 19 -GlucNAc]. The present result introduces GlucNAc rich fraction of F .gigantica that can be used successfully in the diagnosis of acute and chronic fasciolosis.

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Identification and localization of a schistosome-associated fucosyllactose determinant expressed by Fasciola hepatica

Cited by 6 publications

References 26 publications

Estudo comparativo da expressão de carboidratos no sistema ovogranuloma hepático na esquistossomose humana e experimental

Estudo comparativo da expressão de carboidratos no sistema ovogranuloma hepático na esquistossomose humana e experimental

Characterization of fucose-binding lectins in rock- and mud-dwelling snails inhabiting Kuwait Bay

The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

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