Identification and mapping of human papillomavirus type 1 RNA transcripts recovered from plantar warts and infected epithelial cell cultures

Chow, Louise T.; Reilly, Sheila S.; Broker, Thomas R.; Taichman, Lorne B.

doi:10.1128/jvi.61.6.1913-1918.1987

Cited by 79 publications

(24 citation statements)

References 40 publications

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“…This protein is a nonstructural late protein expressed to very high levels in HPV-1induced papillomas (9) and causes the disaggregation of the cytoplasmic skeleton (10). Furthermore, in HPV-1, -6, or -11 condyloma and in low-grade lesions of HPV-16, and E4 mRNA is the most abundant transcript (5,6,8,30), and antibodies to E4 of HPV-8 (33) and of HPV-16 (20) were detected in human sera. The situation with CRPV is somewhat different.…”

Section: Discussionmentioning

confidence: 99%

Progression from papilloma to carcinoma is accompanied by changes in antibody response to papillomavirus proteins

Lin

Borenstein

Selvakumar

et al. 1993

J Virol

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Cottontail rabbit papillomavirus induces benign tumors, papillomas, in rabbits which progress at a high frequency to malignant tumors, carcinomas. Cottontail rabbit papillomavirus therefore provides an experimental model for oncogenic human papillomaviruses. The nature of the antigens recognized by the host has not been identified at any stage of tumor development. Here, we characterized the humoral immune response to viral antigens in cottontail and domestic rabbits at the papilloma stage, in domestic rabbits at the carcinoma stage, and in animals in which papillomas had regressed. Antibodies to linear epitopes were identified by Western blotting (immunoblotting) with bacterial fusion proteins, and evidence for recognition of conformational epitopes was obtained by immunoprecipitation. An immune response to the early proteins El, E2, E6, and E7 was detected only in a fraction of the animals, and all animals were negative for E4 and E5. The response to E6 and E7 peaked around 7 months and then decreased, while that to El and E2 remained level after an initial raise. The antibody response to structural proteins was low at the papilloma stage, and antibodies to Li recognized predominantly conformational epitopes. As papillomas progressed to carcinomas, there was a drastic increase in the response to Li and L2, suggesting a change in interaction between virus-infected host cells and the host's immune system.

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Section: Discussionmentioning

confidence: 99%

Progression from papilloma to carcinoma is accompanied by changes in antibody response to papillomavirus proteins

Lin

Borenstein

Selvakumar

et al. 1993

J Virol

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“…1). E1^E4 mRNA species have been demonstrated to be very abundant in HPV-1-, HPV-6-, and HPV-11-induced condylomas (8,9,24,25), as well as in an HPV-16-transformed rodent cell line (36,37). In addition, an HPV-11-specific E1^E4 transcript was successfully used as a direct marker for HPV-11 infection in both the xenograft system and in cultured human cells (4,33,34).…”

Section: Hpv-16 Infects Human Keratinocytes In Vitro An Hpv-16 Stockmentioning

confidence: 99%

In Vitro Infection and Type-Restricted Antibody-Mediated Neutralization of Authentic Human Papillomavirus Type 16

White¹,

Wilson²,

Bonnez

et al. 1998

J Virol

119

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Human papillomavirus type 16 (HPV-16) is strongly associated with the development of cervical cancer. Studies of model systems with animal papillomaviruses have demonstrated the importance of neutralizing antibodies in preventing papillomavirus-associated disease. The assessment of neutralizing antibody responses against HPV-16, previously hampered by the lack of a viral source, was enabled by the recent propagation of an HPV-16 stock in xenografted severe combined immunodeficiency (SCID) mice. HPV-16 infection of an immortalized human keratinocyte cell line was demonstrated by detection of an HPV-16-specific spliced mRNA amplified by reverse transcriptase PCR. Infection was blocked by preincubation of the virus with antiserum generated against HPV-16 virus-like particles (VLPs) composed of the major capsid protein, L1. To examine potential cross-neutralizing activity among the different genital HPV types, rabbit antisera to L1 VLPs corresponding to HPV-6, -11, -18, -31, -33, -35, -39, and -45 were assayed for the ability to block the HPV-16 infection of cultured cells. Antiserum raised against HPV-33 L1 VLPs was the only heterologous antiserum which inhibited HPV-16 infection. Thus, a neutralization assay for HPV-16 may help to characterize the components required to compose a broadly efficacious genital HPV vaccine.

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“…The upstream regulatory region (URR) contains elements involved in the control of virus transcription and replication (see text for references). (31)(32)(33) suggesting that they can represent but only a small fraction of the papillomavirus transcription products. The present communication reports the discovery of a set of mRNAs from the El gene region of BPV-1 which have not previously been described.…”

Section: Introductionmentioning

confidence: 99%

Messenger RNAs from the E1 region of bovine papillomavirus type 1 detected in virus-infected bovine cells

Burnett¹,

Moreno‐López

Pettersson³

1987

Nucl Acids Res

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Bovine papillomavirus type 1 DNA replicated to a high copy number in virus-infected bovine fibroblasts. Infected bovine cells were therefore used as a source of RNA for Northern blotting analysis to search for viral transcripts hybridizing to the E1 gene region, implicated in viral DNA replication. Cytoplasmic polyadenylated RNA preparations contained at least five different E1-region transcripts, ranging from 1200 to approximately 4500 nucleotides in length. All of these species contained sequence information from the 5'-end of the E1 open reading frame, but only the largest species included sequences from its central portion. The latter transcript is a candidate mRNA for a stimulatory replication factor (R) previously mapped by genetic experiments (1).

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Identification and mapping of human papillomavirus type 1 RNA transcripts recovered from plantar warts and infected epithelial cell cultures

Cited by 79 publications

References 40 publications

Progression from papilloma to carcinoma is accompanied by changes in antibody response to papillomavirus proteins

Progression from papilloma to carcinoma is accompanied by changes in antibody response to papillomavirus proteins

In Vitro Infection and Type-Restricted Antibody-Mediated Neutralization of Authentic Human Papillomavirus Type 16

Messenger RNAs from the E1 region of bovine papillomavirus type 1 detected in virus-infected bovine cells

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