Identification and mechanism analysis of chemical modulators enhancing astaxanthin accumulation in Haematococcus pluvialis

Yu, Xinheng; Niu, Xiangfeng; Zhang, Xiaoqing; Pei, Guangsheng; Liu, Jing; Chen, Lei; Zhang, Weiwen

doi:10.1016/j.algal.2015.07.006

Cited by 46 publications

(7 citation statements)

References 64 publications

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“…This indicates that melatonin enhanced the accumulation of astaxanthin in H. pluvialis in to stressful conditions. Additionally, compared with previously reported induction strategies, astaxanthin production with melatonin treatment combined with nitrogen starvation and photoinduction was much greater than that with H 2 O 2 , butylated hydroxyanisole (BHA), or various light-emitting diode (LED) treatments. − However, because of culture media differences, the dependence of regulators on the algal strain and species and the culture conditions, this observation requires further experimental validation. In H. pluvialis , cyst formation is the primary response to stress.…”

Section: Discussionmentioning

confidence: 96%

Melatonin: A Multifunctional Molecule That Triggers Defense Responses against High Light and Nitrogen Starvation Stress in Haematococcus pluvialis

Ding

Zhao

et al. 2018

J. Agric. Food Chem.

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Melatonin (MLT), a ubiquitously distributed small molecule, functions in plant responses to various biotic and abiotic stresses. However, the interactions between melatonin and other important molecules in Haematococcus pluvialis response stresses are largely unknown. In the present study, exogenous melatonin improved H. pluvialis resistance to nitrogen starvation and high light. We concluded that exogenous melatonin treatment prevented the reactive oxygen species (ROS) burst and limited cell damage induced by abiotic stress through activation of antioxidant enzymes and antioxidants. Astaxanthin, a major antioxidant in H. pluvialis cells, exhibited a 2.25-fold increase in content after treatment with melatonin. The maximal astaxanthin content was 32.4 mg g. The functional roles of the nitric oxide (NO)-mediated mitogen activated protein kinase (MAPK) signaling pathway and cyclic adenosine monophosphate (cAMP) signaling pathway induced by melatonin were also evaluated. The results clearly indicate that cAMP signaling pathways are positively associated with microalgal astaxanthin biosynthesis. Additionally, the NO-dependent MAPK signaling cascade is activated in response to astaxanthin accumulation induced by melatonin, confirming that MAPK is a target of NO action in physiological processes. This work is the first to use H. pluvialis as in vivo model and documents the influence of melatonin on the physiological response to abiotic stress in this microalgae.

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Section: Discussionmentioning

confidence: 96%

Melatonin: A Multifunctional Molecule That Triggers Defense Responses against High Light and Nitrogen Starvation Stress in Haematococcus pluvialis

Ding

Zhao

et al. 2018

J. Agric. Food Chem.

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“…In this study, GA3 is proved to be the most effective factor to promote astaxanthin biosynthesis in heterotrophically-grown C. zofingiensis . GA3 was found to induce astaxanthin biosynthesis in H. pluvialis through up-regulating the transcription of β-carotene ketolase genes, which is involved in converting β-carotene to the canxathathin-the precursor of astaxanthin ( Lu et al, 2010 ; Yu et al, 2015 ). GA is a diterpene phytochrome that control the growth and development of plants, including seed germination, hypocotyl elongation, flower initaiation, stem elongation via enhanced cell division and elongation.…”

Section: Discussionmentioning

confidence: 99%

Effective Two-Stage Heterotrophic Cultivation of the Unicellular Green Microalga Chromochloris zofingiensis Enabled Ultrahigh Biomass and Astaxanthin Production

Chen

et al. 2022

Front. Bioeng. Biotechnol.

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Chromochloris zofingiensis has obtained particular interest as a promising candidate for natural astaxanthin production. In this study, we established a two-stage heterotrophic cultivation process, by using which both the growth of C. zofingiensis and astaxanthin accumulation are substantially enhanced. Specifically, the ultrahigh biomass concentration of 221.3 g L−1 was achieved under the optimum culture conditions in 7.5 L fermenter during 12 days. When scaled-up in the 500 L fermentor, the biomass yield reached 182.3 g L−1 in 9 days, while the astaxanthin content was 0.068% of DW. To further promote astaxanthin accumulation, gibberellic Acid-3 (GA3) was screened from a variety of phytohormones and was combined with increased C/N ratio and NaCl concentration for induction. When C. zofingiensis was grown with the two-stage cultivation strategy, the astaxanthin yield reached 0.318 g L−1, of which the biomass yield was 235.4 g L−1 and astaxanthin content was 0.144% of DW. The content of the total fatty acids increased from 23 to 42% of DW simultaneously. Such an astaxanthin yield was 5.4-fold higher than the reported highest record and surpassed the level of Haematococcus pluvialis. This study demonstrated that heterotrophic cultivation of C. zofingiensis is competitive for industrial astaxanthin production.

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“…Scientists showed that cytokine(zeatin) has not any effect on Astaxanthin accumulation in H.pluvialis. (Yu et al, 2015). Some scientists showed that cytokines in forty-five (45) microalgal species increased cell division, growth processes, and photosynthetic activity, also they had a cellular protective role.…”

Section: Cytokininsmentioning

confidence: 99%

The Enhancement of Astaxanthin Production Using Medium Cultures Components, Stressors, Nanoparticles, Elicitors, and Gene Engineering in Haematococcus pluvialis

Garshasbi¹

2023

Preprint

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Natural astaxanthin is a precious substance obtained from some organisms such as microalgae. This plant has many benefits for humans, so research into its cost-effective and economical production has recently increased. For this purpose, some methods such as the use of different culture media, gene engineering, different stresses, nanoparticles, bio acids, and phytohormones are important. Accordingly, this review study was conducted to demonstrate the effect of the factors mentioned above for the high production of astaxanthin in microalgae, especially Haematococcus pluvialis (H.p).

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Identification and mechanism analysis of chemical modulators enhancing astaxanthin accumulation in Haematococcus pluvialis

Cited by 46 publications

References 64 publications

Melatonin: A Multifunctional Molecule That Triggers Defense Responses against High Light and Nitrogen Starvation Stress in Haematococcus pluvialis

Melatonin: A Multifunctional Molecule That Triggers Defense Responses against High Light and Nitrogen Starvation Stress in Haematococcus pluvialis

Effective Two-Stage Heterotrophic Cultivation of the Unicellular Green Microalga Chromochloris zofingiensis Enabled Ultrahigh Biomass and Astaxanthin Production

The Enhancement of Astaxanthin Production Using Medium Cultures Components, Stressors, Nanoparticles, Elicitors, and Gene Engineering in Haematococcus pluvialis

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