Identification and molecular analysis of races of Fusarium oxysporum f. sp. lycopersici isolated from tomato in Baja California, Mexico

Armenta-López, Sara Elodia; Valenzuela-Solano, César; Hernández-Martínez, Rufina

doi:10.18781/r.mex.fit.2103-4

Cited by 4 publications

(4 citation statements)

References 25 publications

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“…lycopersici (Fol) was procured from Laboratorio de Biotecnología Vegetal y Postcosecha (CIAD, Hermosillo, México). The fungus was isolated from tomato fruit (Solanum lycopersicum) and was identified according to the morphological and microscopic characteristics reported [19,20].…”

Section: Methodsmentioning

confidence: 99%

Arabinoxylans Release from Brewers’ Spent Grain Using Extrusion and Solid-State Fermentation with Fusarium oxysporum and the Antioxidant Capacity of the Extracts

Cervantes-Ramirez

Vásquez-Lara

Sánchez‐Estrada

et al. 2022

Foods

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Brewers’ spent grain (BSG) is the most abundant byproduct generated from the beer-brewing process. BSG is a material rich in hemicellulose, composed of arabinoxylans (AX). However, the high crosslinking of this material causes low availability of AX, for which it is necessary to apply different treatments. The objective of this research is to increase the release of arabinoxylans through solid-state fermentation with Fusarium oxysporum f. sp. lycopersici using extruded brewery spent grain. First, the BSG is subjected to two types of physical treatments: extrusion at 20% moisture, 200 rpm and 50 °C (BSGe), and blade milling (BSGm). The chemical composition is determined for each sample (BSG, BSGe and BSGm). Subsequently, the solid-state fermentation process (SSF) is carried out on each sample. The fermentation kinetics at 30 °C are monitored for 7 days. Once the SSF concludes, AX are extracted, and the purity of AX is determined by the phloroglucinol colorimetric assay. Finally, the total phenolic compounds, phenolic acids and antioxidant capacity by DPPH are quantified. No significant differences (p ≥ 0.05) in the protein, lipid, ash or total dietary fiber contents are found among the samples. No significant difference (p ≥ 0.05) in the content of soluble fiber is found, although BSGe and BSGm have higher values than BSG. On the other hand, the yields of soluble AX exhibit significant differences (p ≤ 0.05) among nonfermented samples (BSG, 0.03%; BSGm, 0.53%; BSGe, 0.70%) and with SSF (BSG, 2.95%; BSGm, 6.24%; and BSGe, 9.58%). In addition, the contents of free phenolic compounds and free phenolic acids and the percent inhibition of free extracts by 2,2-diphenyl-1-picrylhydrazyl (DPPH) differ significantly (p ≤ 0.05) between samples subjected to SSF and nonfermented samples. Therefore, extrusion and SSF treatment increase AX release from BSG as well as the antioxidant capacity of the extracts.

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Section: Methodsmentioning

confidence: 99%

Arabinoxylans Release from Brewers’ Spent Grain Using Extrusion and Solid-State Fermentation with Fusarium oxysporum and the Antioxidant Capacity of the Extracts

Cervantes-Ramirez

Vásquez-Lara

Sánchez‐Estrada

et al. 2022

Foods

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“…Fusarium oxysporum is a phytopathogen with some strain that causes vascular wilting, crown, and root rots in several crops [1].…”

Section: Extended Abstractmentioning

confidence: 99%

Computational Design of a Biosensor Based On Silicon Dioxide Nanoneedles Selective To Fusarium Oxysporum

Zarazúa-Sánchez,

Díaz-Cervantes,

Aguilera-Granja

2024

Proceedings of the 9th World Congress on Recent Advances in Nanotechnology

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“…Due to these challenges, in planta-based methods continue to provide the standard for the accurate differentiation of pathogenic from nonpathogenic strains, segregation of the different formae specialis, and differentiation of Fol races (e.g., Loṕez-Benıtez et al, 2018;Cabral et al, 2020;Munawar et al, 2020;Armenta-Loṕez et al, 2021;de Oliveira et al, 2021). In planta identification methods vary in terms of plant age, inoculation method, and the duration needed for symptom development, with results in 2-12 weeks, depending on the method.…”

Section: Introductionmentioning

confidence: 99%

Monitoring for a new I3 resistance gene-breaking race of F. oxysporum f. sp. lycopersici (Fusarium wilt) in California processing tomatoes following recent widespread adoption of resistant (F3) cultivars: Challenges with race 3 and 4 differentiation methods

et al. 2023

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Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici (Fol), causes losses in tomato production worldwide, with major impacts on Californian tomato processing. Single-gene resistance is the primary management tool, but its efficacy has been compromised following the emergence of two successive resistance-breaking races, which, in California, emerged within 12 years of resistance deployment. Fol race 3-resistant (F3) processing tomato cultivars (containing the I3 resistance gene) were deployed in the state starting in approximately 2009. The emergence of a new resistance-breaking race (which would be called race 4) is imminent, and early detection will be critical to delay the spread while new resistance is sought. The detection of Fol race 4 is challenged by the lack of validated, rapid, and accurate diagnostic tools. In evaluating in planta phenotyping methods, this study found that rapid seedling phenotyping is not reliable and generates false positives for nonpathogens. Longer (10 weeks) mature plant assays are the most reliable, but may not be sufficiently timely. As an additional challenge, based on field and greenhouse studies, Fol race 3 can cause symptoms in resistant F3 cultivars at frequencies greater (30%) than expected for off-types (<2%). We developed a three-F3 cultivar in planta assay to overcome the challenges this posed to differentiating Fol race 3 and Fol race 4. Using the assay, we determined that all putative resistance-breaking cases were Fol race 3; Fol race 4 was not detected in these early survey efforts. These results highlight the need for developing rapid Fol race 4 detection tools and a better understanding of the factors underlying inconsistent I3 gene expression in Fol race 3.

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Identification and molecular analysis of races of Fusarium oxysporum f. sp. lycopersici isolated from tomato in Baja California, Mexico

Cited by 4 publications

References 25 publications

Arabinoxylans Release from Brewers’ Spent Grain Using Extrusion and Solid-State Fermentation with Fusarium oxysporum and the Antioxidant Capacity of the Extracts

Arabinoxylans Release from Brewers’ Spent Grain Using Extrusion and Solid-State Fermentation with Fusarium oxysporum and the Antioxidant Capacity of the Extracts

Computational Design of a Biosensor Based On Silicon Dioxide Nanoneedles Selective To Fusarium Oxysporum

Monitoring for a new I3 resistance gene-breaking race of F. oxysporum f. sp. lycopersici (Fusarium wilt) in California processing tomatoes following recent widespread adoption of resistant (F3) cultivars: Challenges with race 3 and 4 differentiation methods

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