Identification and Molecular Characteristics of a Novel Single-Stranded RNA Virus Isolated from Culextritaeniorhynchus in China

Wu, Zhen; Liu, Jingyu; Feng, Xiao; Zhang, Yuli; Liu, Lin; Niu, Guoyu

doi:10.1128/spectrum.00536-23

Cited by 3 publications

References 22 publications

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Identification of a newly discovered virus from Culex and Armigeres mosquitoes in China

Zhu,

Chen,

Sun

et al. 2024

Sci Rep

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Mosquito associated virus have always been a significant threat to global health. Metagenomics offers a straightforward and quantitative means to acquire the information of novel virus and has greatly enriched the content of mosquito associated virus databases. During an entomological surveillance for arthropod-borne viruses in China, we identified a previously unrecognized virus from mosquitoes, temporarily named Huajieling virus. In this study, a total of 3,960 mosquitoes were collected and then divided into 91 pools, according to location and species. QRT-PCR and nested PCR were performed to confirm the presence of Huajieling virus. Its genomic features and phylogenetic relationships were further analyzed. Our results showed that Huajieling virus was detected in 7 of the 91 mosquito pools and that the minimum infection rate (MIR) was 0.18% (7/3,960). One complete genome sequence and 2 viral partial sequences were obtained from the Huajieling virus-positive pools. Pairwise distances analysis indicated that these amplified sequences shared high nucleotide identity. Phylogenetic analysis demonstrated that Huajieling virus is most closely related to Wufeng shrew picorna-like virus 43, which belonging to Picornavirales . Further analyses indicated that Huajieling virus is a new member of unclassified Picornavirales , and is intermediate between the family Caliciviridae and Secoviridae in taxonomic status.

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Identification of a newly discovered virus from Culex and Armigeres mosquitoes in China

Zhu,

Chen,

Sun

et al. 2024

Sci Rep

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Expanding the Plant Virome: Umbra-Like Viruses Use Host Proteins for Movement

Simon,

Quito-Avila,

Bera

2024

Annual Review of Virology

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Before the very recent discovery of umbra-like viruses (ULVs), the signature defining feature of all plant RNA viruses was the encoding of specialized RNA-binding movement proteins (MPs) for transiting their RNA genomes through gated plasmodesmata to establish systemic infections. The vast majority of ULVs share umbravirus-like RNA-dependent RNA polymerases and 3′-terminal structures, but they differ by not encoding cell-to-cell and long-distance MPs and by not relying on a helper virus for trans-encapsidation and plant-to-plant transmission. The recent finding that two groups of ULVs do not necessarily encode MPs is expanding our understanding of the minimum requirements for modern plant RNA viruses. ULV CY1 from citrus uses host protein PHLOEM PROTEIN 2 (PP2) for systemic movement, and related ULVs encode a capsid protein, thereby providing an explanation for the lack of helper viruses present in many ULV-infected plants. ULVs thus resemble the first viruses that infected plants, which were likely deposited from feeding organisms and would have similarly required the use of host proteins such as PP2 to exit initially infected cells.

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Huajieling virus: A newly discovered member of the Caliciviridae family from mosquito, China

zhu,

Chen,

et al. 2024

Preprint

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Mosquito associated virus have always been a significant threat to global health. Metagenomics offers a straightforward and quantitative means to acquire the information of novel virus and has greatly enriched the content of mosquito associated virus databases. During an entomological surveillance for arthropod-borne viruses in China, we identified a previously unrecognized virus from mosquitoes, temporarily named Huajieling virus. In this study, a total of 3,960 mosquitoes were collected and then divided into 91 pools, according to location and species. QRT-PCR and nested PCR were performed to confirm the presence of Huajieling virus. Its genomic features and phylogenetic relationships were further analyzed. Our results showed that Huajieling virus was detected in 7 of the 91 mosquito pools and that the minimum infection rate (MIR) was 0.18% (7/3,960). One complete genome sequence and 2 viral partial sequences were obtained from the Huajieling virus-positive pools. Pairwise distances analysis indicated that these amplified sequences shared high nucleotide identity. Phylogenetic analysis demonstrated that Huajieling virus is most closely related to Wufeng shrew picorna like virus 43, which belonging to Caliciviridae. Further analyses indicated that Huajieling virus is a new member of unclassified Caliciviridae.

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Identification and Molecular Characteristics of a Novel Single-Stranded RNA Virus Isolated from Culextritaeniorhynchus in China

Abstract: Our study provided important epidemiological evidence for the epidemic of HMV2 in Shandong Province. Here, we report the first isolation and molecular characteristics of this virus and enrich our knowledge of the distribution of HMV2 in China.

Cited by 3 publications

References 22 publications

Identification of a newly discovered virus from Culex and Armigeres mosquitoes in China

Identification of a newly discovered virus from Culex and Armigeres mosquitoes in China

Expanding the Plant Virome: Umbra-Like Viruses Use Host Proteins for Movement

Huajieling virus: A newly discovered member of the Caliciviridae family from mosquito, China

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