Identification and Molecular Characterization of Class 1 Integrons in Multiresistant Escherichia coli Isolates from Poultry Litter

Ponce-Rivas, Elizabeth; Muñoz-Márquez, María-Enriqueta; Khan, Ashraf A.

doi:10.1128/aem.00660-12

Cited by 11 publications

(9 citation statements)

References 19 publications

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“…Sedentary integrons are DNA elements found in the chromosomes of many species and were initially discovered due to their association with AMR (Mazel, 2006;Partridge et al, 2009). In contrast to sedentary integrons, mobile resistance integrons are often found in plasmids (Ponce-Rivas et al, 2012). The role of these elements in the acquisition and dissemination of ARGs is crucial, especially in Gram-negative bacteria (Ponce-Rivas et al, 2012), but they are also present in Gram-positive bacteria (Nandi et al, 2004).…”

Section: Integronsmentioning

confidence: 99%

“…In contrast to sedentary integrons, mobile resistance integrons are often found in plasmids (Ponce-Rivas et al, 2012). The role of these elements in the acquisition and dissemination of ARGs is crucial, especially in Gram-negative bacteria (Ponce-Rivas et al, 2012), but they are also present in Gram-positive bacteria (Nandi et al, 2004). Integrons contain the gene encoding integrase (IntI), an enzyme that allows the incorporation of circular DNA segments by site-specific recombination (Cambray et al, 2010).…”

Section: Integronsmentioning

confidence: 99%

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Targeting Plasmids to Limit Acquisition and Transmission of Antimicrobial Resistance

et al. 2020

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Antimicrobial resistance (AMR) is a significant global threat to both public health and the environment. The emergence and expansion of AMR is sustained by the enormous diversity and mobility of antimicrobial resistance genes (ARGs). Different mechanisms of horizontal gene transfer (HGT), including conjugation, transduction, and transformation, have facilitated the accumulation and dissemination of ARGs in Gram-negative and Gram-positive bacteria. This has resulted in the development of multidrug resistance in some bacteria. The most clinically significant ARGs are usually located on different mobile genetic elements (MGEs) that can move intracellularly (between the bacterial chromosome and plasmids) or intercellularly (within the same species or between different species or genera). Resistance plasmids play a central role both in HGT and as support elements for other MGEs, in which ARGs are assembled by transposition and recombination mechanisms. Considering the crucial role of MGEs in the acquisition and transmission of ARGs, a potential strategy to control AMR is to eliminate MGEs. This review discusses current progress on the development of chemical and biological approaches for the elimination of ARG carriers.

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Section: Integronsmentioning

confidence: 99%

Section: Integronsmentioning

confidence: 99%

Targeting Plasmids to Limit Acquisition and Transmission of Antimicrobial Resistance

et al. 2020

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“…In one of the studies on multiresistant poultry E . coli isolates it was found that resistance determinants were harboured in class 1 integron [ 42 ]. Most common carriages were observed for aadA1 and dfrA1 gene cassettes, encoding resistance to streptomycin and trimethoprim, which were either located on mega-plasmids or on the chromosome.…”

Section: Discussionmentioning

confidence: 99%

An Escherichia coli Strain, PGB01, Isolated from Feral Pigeon Faeces, Thermally Fit to Survive in Pigeon, Shows High Level Resistance to Trimethoprim

et al. 2015

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In this study, of the hundred Escherichia coli strains isolated from feral Pigeon faeces, eighty five strains were resistant to one or more antibiotics and fifteen sensitive to all the antibiotics tested. The only strain (among all antibiotic-resistant E. coli isolates) that possessed class 1 integron was PGB01. The dihydrofolate reductase gene of the said integron was cloned, sequenced and expressed in E. coli JM109. Since PGB01 was native to pigeon’s gut, we have compared the growth of PGB01 at two different temperatures, 42°C (normal body temperature of pigeon) and 37°C (optimal growth temperature of E. coli; also the human body temperature), with E. coli K12. It was found that PGB01 grew better than the laboratory strain E. coli K12 at 37°C as well as at 42°C. In the thermal fitness assay, it was observed that the cells of PGB01 were better adapted to 42°C, resembling the average body temperature of pigeon. The strain PGB01 also sustained more microwave mediated thermal stress than E. coli K12 cells. The NMR spectra of the whole cells of PGB01 varied from E. coli K12 in several spectral peaks relating some metabolic adaptation to thermotolerance. On elevating the growth temperature from 37°C to 42°C, susceptibility to kanamycin (both strains were sensitive to it) of E. coli K12 was increased, but in case of PGB01 no change in susceptibility took place. We have also attempted to reveal the basis of trimethoprim resistance phenotype conferred by the dfrA7 gene homologue of PGB01. Molecular Dynamics (MD) simulation study of docked complexes, PGB01-DfrA7 and E. coli TMP-sensitive-Dfr with trimethoprim (TMP) showed loss of some of the hydrogen and hydrophobic interaction between TMP and mutated residues in PGB01-DfrA7-TMP complex compared to TMP-sensitive-Dfr-TMP complex. This loss of interaction entails decrease in affinity of TMP for PGB01-DfrA7 compared to TMP-sensitive-Dfr.

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“…[6] By amplifying, for example, the variable region of class 1 integrons from community DNA and subsequent Southern blot hybridization, Binh et al [49] demonstrated that aadA gene cassettes were introduced via manure into agricultural soils. Ponce-Rivas et al [50] used PCR to evaluate the prevalence and origin of class 1 integrons and Qnr determinants in fluoroquinolone-resistant Escherichia coli isolates from poultry litter. They showed that resistance determinants within E. coli of poultry origin are genetically diverse and suggested the need for surveillance programs focused on the detection of genetic elements related to horizontal transfer genes.…”

Section: Pcr-based Approachesmentioning

confidence: 99%

Approaches to Assess the Effects and Risks of Veterinary Antibiotics Applied with Manure to Soil

Jechalke¹,

Cook²,

Smalla³

2015

Antimicrobial Resistance - An Open Challenge

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In veterinary medicine, large quantities of antibiotic substances are administered each year for therapeutic and prophylactic purposes or to promote growth. "s a consequence, the antibiotics and bacteria carrying transferable antibiotic resistance genes are excreted by the animals and reach the environment through run-off, leaching, and/or following manure application to agricultural fields, where they have been found to affect the structure and function of soil bacterial communities. However, we are only beginning to understand the global effects of environmental pollution with antibiotics and resistance determinants and the resulting risks for human health. For regulatory purposes, there is urgent need for criteria and methods that allow reliable and reproducible assessment of risks associated with release of realistic concentrations of antibiotics and resistance determinants into the environment following manure application. In this chapter, we will summarize recent advances, limitations, and research needed to optimize the methods to quantify and evaluate the effects and risks associated with these compounds. "pproaches that are discussed focus on antibiotic resistance genes and include classical tools such as cultivation and PCR detection as well as quantitative real-time PCR and next-generation sequencing technologies used in combination with functional screening.

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Identification and Molecular Characterization of Class 1 Integrons in Multiresistant Escherichia coli Isolates from Poultry Litter

Cited by 11 publications

References 19 publications

Targeting Plasmids to Limit Acquisition and Transmission of Antimicrobial Resistance

Targeting Plasmids to Limit Acquisition and Transmission of Antimicrobial Resistance

An Escherichia coli Strain, PGB01, Isolated from Feral Pigeon Faeces, Thermally Fit to Survive in Pigeon, Shows High Level Resistance to Trimethoprim

Approaches to Assess the Effects and Risks of Veterinary Antibiotics Applied with Manure to Soil

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