Identification and Molecular Characterization of a Bovine G3 Rotavirus Which Causes Age-Independent Diarrhea in Cattle

Abstract

G3 rotaviruses have been reported rarely in cattle, and none have been characterized. We report the first genomic characterization of a bovine G3 rotavirus, CP-1, which had been biologically characterized in vivo and shown to cause age-independent diarrhea. CP-1 was a G3 rotavirus as its VP7 had 92 to 96% deduced amino acid identity to those of G3 rotaviruses. However, initially, CP-1 was identified as a G10 rotavirus by RT-PCR even though the CP-1 VP7 had only 81 to 85% deduced amino acid identity to those of G10 rotaviruses. Rotavirus CP-1 was of P[5] specificity, a type common in cattle, and had a bovine NSP1 and NSP4. These results added another animal species to those in which G3 rotaviruses have been found, characterized a bovine rotavirus which caused age-independent diarrhea in calves, and raised the possibility that bovine G3 rotaviruses may be misdiagnosed as G10 rotaviruses.

Molecular characterization of group A bovine rotavirus in southeastern and central-western Brazil, 2009-2010

“…Genotype G6 was the most frequent and was identiϐied in 17 (42.5%) samples, followed by genotypes G8, G10 and G11, which were detected in ϐive (12.5%), two (5%) and one (2.5%) sample (s), respectively. (Brito et al 2000) and in other countries (Falcone et al1999, Attar et al 2002, Garaicoechea et al 2006, infections in calves caused by group A rotaviruses belonging to other G and P genotypes have also been reported (Abe et al 2011). …”

Detection of Rare G3P[19] Porcine Rotavirus Strains in Chiang Mai, Thailand, Provides Evidence for Origin of the VP4 Genes of Mc323 and Mc345 Human Rotaviruses

“…The G3 and G4 genotypes were copredominant genotypes, with a prevalence of 43.6% and 46.2%, respectively, followed by much less prevalent G5 (5.1%) and G2 (2.6%) genotypes. Most of the G4 genotypes (17 of 18 isolates) were found in combination with P [6], while the re-maining one was with P [13]. The majority of G3 genotypes (13 of 17 isolates) were found in combination with P [19], the rare P genotype of porcine rotaviruses, whereas another three isolates were in combination with P [6].…”

“…The specificity of the VP4P19 primer was evaluated by testing the primer with all 13 isolates of P [19], three isolates of P [13], and one each of P [3], P [4], and P [12]. Briefly, 3 l of RNA genome was added to 0.3 l of 50% dimethyl sulfoxide before being heated at 97°C for 5 min and then rapidly cooled on ice.…”

“…However, other porcine rotaviruses, such as G1, G2, G6, G8, G9, and G10, have also been reported occasionally (1,5,6,19,25,32,34,43,45,52,57). For the P type, P [6] and P [7] were found to be the most common genotypes in pigs, while other P types, such as P [13], P [14], P [19], P [23], and P [26], were seldom reported (3,14,20,25,27,30,31,59). In Thailand, the epidemiological study of the group A rotavirus in pigs has been limited, and G3 had been the only G type detected in the last decade (44), until G10 was recently reported (43).…”

Molecular Analysis of the VP7, VP4, VP6, NSP4, and NSP5/6 Genes of a Buffalo Rotavirus Strain: Identification of the Rare P[3] Rhesus Rotavirus-Like VP4 Gene Allele

“…Rotavirus strains belonging to serotypes G6, G8, and G10, in association with VP4 types P6 [1], P7 [5], and P8 [11], are commonly found in cattle, though strains belonging to G1, G2, G3, and G11 have been detected sporadically (18,36,57). An unusual P [17]-and G7-like bovine rotavirus has also been isolated from a calf, presumably the result of interspecies transmission from birds to cows (55).…”