Identification and molecular characterization of begomovirus and associated satellite DNA molecules infecting Cyamopsis tetragonoloba

Kumar, Jitendra; Kumar, Abhinav; Roy, Joy; Tuli, Rakesh; Khan, J. A.

doi:10.1007/s11262-010-0482-7

Cited by 46 publications

(22 citation statements)

References 28 publications

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“…Although alphasatellites are capable of autonomous replication in permissible plant cells, they depend on helper virus-encoded proteins for movement within and between plants (8). The two alphasatellites were maintained in wheat by WDIV as efficiently as by the begomoviruses (CLCuBuV and CyTLCuV) in tobacco and are thus typical of the earlier described satellites (38,46). RNA silencing is an antiviral defense mechanism.…”

Section: Discussionmentioning

confidence: 79%

“…The primer pair MF1_FOR/REV (Table 1) was used in the PCR assays to amplify the WDIV genome from wheat samples. Alphasatellites and betasatellite were amplified in PCR assays using the primer pairs nanofor/nanorev and ␤01/04 (Table 1), respectively, as described previously (38). The RCA and PCR assays were performed for all of the 460 wheat (450 symptomatic and 10 asymptomatic) samples.…”

Section: Methodsmentioning

confidence: 99%

“…Both of these alphasatellites were ϳ1.3 kb long and shared 77% identity with one another. In a similarity search, one alphasatellite showed an identity of 99% with Cotton leaf curl Multan alphasatellite (CLCuMA) from okra (46), and the other showed an identity of 93% with Guar leaf curl alphasatellite (GLCuA) from Cyamopsis tetragonoloba (38), thus establishing the presence of CLCuMA and GLCuA in wheat. Both of these alphasatellite molecules possessed three highly conserved features: a loop sequence TAGTATTAC (nonanucleotide sequence), an adenine (A)-rich region, and a gene encoding a Rep (Fig.…”

Section: Detection Of Wdiv and Satellites In Wheatmentioning

confidence: 99%

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Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Kumar

Singh

et al. 2014

J Virol

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In contrast to begomoviruses, mastreviruses have not previously been shown to interact with satellites. This study reports the first identification of the association of satellites with a mastrevirus in field-grown plants. Two alphasatellite species were detected in different field samples of wheat infected with Wheat dwarf India virus (WDIV), a Cotton leaf curl Multan alphasatellite (CLCuMA) and a Guar leaf curl alphasatellite (GLCuA). In addition to the alphasatellites, a betasatellite, Ageratum yellow leaf curl betasatellite (AYLCB), was also identified in the wheat samples. No begomovirus was detected in the wheat samples, thus establishing association of the above-named satellites with WDIV. Agrobacterium-mediated inoculation of WDIV in wheat, in the presence of either of the alphasatellites or the betasatellite, resulted in infections inducing more severe symptoms. WDIV efficiently maintained each of the alphasatellites and the betasatellite in wheat. The satellites enhanced the level of WDIV DNA in wheat. Inoculation of the satellites isolated from wheat with various begomoviruses into Nicotiana tabacum demonstrated that these remain capable of interacting with the viruses with which they were first identified. Virus-specific small RNAs accumulated in wheat upon infection with WDIV but were lower in abundance in plants coinfected with the satellites, suggesting that both the alphasatellites and the betasatellite suppress RNA silencing. These results suggest that the selective advantage for the maintenance of the alphasatellites and the betasatellite by WDIV in the field is in overcoming RNA silencing-mediated host defense. IMPORTANCE Wheat is the most widely cultivated cereal crop in the world. A number of viruses are important pathogens of wheat, including the viruses of the genus

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Section: Discussionmentioning

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Detection Of Wdiv and Satellites In Wheatmentioning

confidence: 99%

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Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Kumar

Singh

et al. 2014

J Virol

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“…The alphasatellite molecules are also associated with the begomovirus-induced diseases occurring in the Indian subcontinent [35,36]. Like the betasatellites, the alphasatellite associated with TbLCD in Pusa, Bihar, was also found to be a recombinant of major and minor parents spreading in the Indian sub-continent (Fig.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Tobacco leaf curl Pusa virus, a new monopartite Begomovirus associated with tobacco leaf curl disease in India

Singh

Haq

et al. 2011

Virus Genes

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Leaf curl disease of tobacco (TbLCD) is endemic in India. A monopartite Begomovirus, a betasatellite and an alphasatellite were found associated with the disease in Pusa, Bihar. The DNA-A of the Begomovirus associated with TbLCD in Pusa, Bihar was found to comprise of 2707 nt with a typical Old World begomovirus-like genome organization. The full-length sequence of DNA-A [HQ180391] showed that the Pusa isolate is a newly described member of the genus Begomovirus, as it had <89% sequence homology with DNA-A of all the known begomoviruses. The isolate is tentatively named as Tobacco leaf curl Pusa virus [India:Pusa:2010]. The betasatellite (HQ180395) associated with TbLCD in Pusa was identified as a variant of Tomato leaf curl Bangladesh betasatellite [IN:Raj:03], with which it shared 90.4% sequence identity. The alphasatellite (HQ180392) associated with the disease had highest 87% nucleotide sequence identity with Tomato leaf curl alphasatellite. The Begomovirus, betasatellite, and alphasatellite associated with TbLCD in Pusa, Bihar, India were found to be recombinants of extant begomoviruses, betasatellites and alphasatellites spreading in the Indian sub-continent and South-East Asia.

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“…Ltd. Bangalore, India) as per the manufacturer's instruction and used as template for amplification of betasatellite DNA in PCR. Primer pair b01 (5 0 -GAAACCACTACGCTACG CAGCAGCC-3 0 ) and b04 (5 0 -ACCCTCCCAGGGGTA CACACCGCCG-3 0 ) [18] were used and PCR was performed at 94°C/5 min for one cycle as initial denaturation, 94°C/45 s, 50°C/60 s, and 72°C/90 s for 30 cycles and a final amplification 72°C/5 min. PCR yielded a DNA fragment of ca.…”

Section: Isolation Of Total Dna and Confirmation Of Begomoviral Complexmentioning

confidence: 99%

Study of betasatellite molecule from leaf curl disease of sunn hemp (Crotalaria juncea) in India

Kumar

Khan

et al. 2010

Virus Genes

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Leaves of sunn hemp (Crotalaria juncea) showing geminiviral symptoms were collected from Lucknow, India during rainy season in 2008. DNA template isolated from the symptomatic leaf tissues were subjected to polymerase chain reaction (PCR) using specific primers to amplify coat protein (CP) gene of DNA-A as well as betasatellite DNA associated with the leaf curl disease. CP gene showed 97% sequence identity with that of Cotton leaf curl Burewala virus (CLCuBwV). Further, the betasatellite DNA molecule revealed sequence similarity with previously characterized betasatellite DNA of begomoviruses affecting malvaceous crops from different regions of India and Pakistan. Maximum similarity (>90%) of betasatellite DNA under study was observed with Cotton leaf curl Multan betasatellite (CLCuMB-[Pak: Mul17:08) and other betasatellite DNA from Pakistan thus confirming possible infection of C. juncea with begomovirus. A complementary sense open reading frame (ORF) βC1 is present at nucleotide position 194-550. Sequence comparison of this ORF with other members of begomoviruses further confirmed association of a begomovirus with C. juncea. The betasatellite DNA when expressed under the control of CaMV35S promoter Nicotiana tabacum, showed leaf deformities. Our results demonstrated that a malvaceous betasatellite is adapted by a nonmalvaceous host and causes similar disease symptoms.

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Identification and molecular characterization of begomovirus and associated satellite DNA molecules infecting Cyamopsis tetragonoloba

Cited by 46 publications

References 28 publications

Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Molecular characterization of Tobacco leaf curl Pusa virus, a new monopartite Begomovirus associated with tobacco leaf curl disease in India

Study of betasatellite molecule from leaf curl disease of sunn hemp (Crotalaria juncea) in India

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