Identification and molecular characterization of Bean yellow mosaic virus infecting French bean in Himachal Pradesh

Sharma, Vivek; Sharma, Anuradha; Rajput, Kajal; Sharma, Shalini

doi:10.1007/s13337-015-0270-z

Cited by 18 publications

(6 citation statements)

References 13 publications

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“…Daha önce yapılan çalışmalarda da DAS ELISA yöntemi kullanılmış ve değişen oranlarda bulaşıklık değerleri belirlenmiştir [6,11,18,19] Burdur'da yetiştirilen fasulye üretim alanlarında BYMV'nün varlığının ilk kez ortaya konulduğu bu araştırma sonuçları daha sonraki çalışmalara ışık tutacaktır. Fasulye bitkilerini virüs hastalıklarından korumada etkili bir strateji olan dayanıklılık çalışmalarına ağırlık verilmelidir.…”

Section: şEkil 1 Klorotik Beneklenme Mozayik Ve şEkil Bozukluğu Belunclassified

Burdur İlinde Yetiştirilen Fasulye Bitkilerinde Bean Yellow Mosaic Virus (BYMV)’ünün Araştırılması

Ulum¹,

Kiliç

Yardımcı³

2020

Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Bean yellow mosaic virus, Teşhis Özet: Bu çalışmada Türkiye'deki fasulye yetiştiriciliğinde önemli bir paya sahip olan Burdur ilinde BYMV (Bean yellow mosaic virus)'ün varlığı araştırılmıştır. Öncelikli olarak bu bölgedeki fasulye üretim alanlarına sürveyler yapılarak virüs simptomu sergileyen fasulye yaprak örnekleri alınmıştır. Toplanan fasulye yaprak örneklerinde BYMV'nin varlığı biyolojik ve serolojik olarak araştırılmıştır. Arazi çalışmaları sonucunda; 443 örnek toplanmış ve bütün örnekler BYMV'ne spesifik antiserumlar kullanılarak double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) yöntemiyle testlenmiştir. DAS-ELISA testi sonucunda 443 örnekten 97'sinde (% 21.89) BYMV belirlenmiştir. DAS-ELISA testi sonucunda pozitif reaksiyon veren yaprak örnekleri, test bitkilerine inokule edilmiş ve oluşan belirtiler değerlendirilmiştir. Çalışmaların sonucunda Burdur fasulye üretim alanlarından alınan yaprak örneklerinde BYMV enfeksiyonları saptanmıştır.

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Section: şEkil 1 Klorotik Beneklenme Mozayik Ve şEkil Bozukluğu Belunclassified

Burdur İlinde Yetiştirilen Fasulye Bitkilerinde Bean Yellow Mosaic Virus (BYMV)’ünün Araştırılması

Ulum¹,

Kiliç

Yardımcı³

2020

Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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“…The integrity of total RNA was analyzed on 1% denaturing gel using leaves from healthy plants as negative control. cDNA was synthesized using reverse transcriptase in a reaction volume of 20 µl, containing 1 µg of total RNA, 1 µl of oligodT (10)(11)(12)(13)(14)(15)(16)(17)(18) as per the manufacturer's instructions. For reverse transcription-polymerase chain reaction (RT-PCR), BYMV specific primer pair BYMV-pnsF (5`-TCAGATCAAGAGCAACTCAATGCA-3`) and BYMV-pnsR (5`-GACGGATACTCTAAATACGAACA-3`) [17] specifically designed from the BYMV coat protein gene.…”

Section: Detection Of Bymv Coat Protein Gene By Rt-pcrmentioning

confidence: 99%

“…Agarose gels (1.2%), used for resolving the PCR products, were prepared and run as described by Maniatis et al The gel was stained with ethidium bromide and evaluated under UV light. The sizes of PCR products were estimated according to the migration pattern of a 100 bp DNA ladder [18].…”

Section: Detection Of Bymv Coat Protein Gene By Rt-pcrmentioning

confidence: 99%

Molecular Detection of Bean Yellow Mosaic Virus in Lupinus albus Plants and its Associated Alterations in Biochemical and Physiological Parameters

Barakat¹,

Torky²

2017

J Antivir Antiretrovir

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Bean yellow mosaic virus is one of the most devastating diseases of cultivated Leguminosae plants worldwide causing mosaic, mottling, malformation and distortion in infected cultivar plants. Present study was conducted to investigate the possibility of infection of Lupinus albus (Lupine) with Bean yellow mosaic virus. Virus isolate was identified by detection of the coat protein gene amplified by reverse transcription polymerase chain reaction and also via Chenopodium Amaranticolor as a diagnostic host plant. Results showed that infection can be induced under greenhouse conditions and infected plants showed a considerable level of mosaic symptoms. As disease development in infected plants is always associated with physiological and chemical changes, some metabolic alterations parameters have been evaluated like photosynthetic pigment contents, total carbohydrate content, total soluble protein, total protein, total free amino acid, proline induction, total phenolics, salicylic acid, and abscisic acid content in healthy and infected lupine plants. Results showed a great variation in all the biochemical categories in Lupinus albus infected with bean yellow mosaic virus as compared to healthy plants. Chlorophyll a of virus inoculated Lupinus albus decreased to 27%, whereas Chlorophyll b content decreased to 19.5% and carbohydrate content decreased to 36% when compared to healthy control plant corresponding values. Results also showed many metabolic changes in virus infected Lupine plants. The effect of virus infection on the induction of plant growth regulators like abscisic acid was determined, as well as the relationship between abscisic acid activation, accumulation of the virus, and symptoms development was discussed, and the effect of abscisic acid inhibitor application on virus infection and Lupine primary and secondary metabolism was elucidated, as this effect is a neglected field of research.

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“…2 Bean yellow mosaic virus (BYMV) belongs to the Potyvirus genus (Family: Potyviridiae) and is a devastating viral disease for many crop legumes and ornamental plants throughout the world. 3 BYMV disease poses a significant threat for faba bean production, with losses of 30-50%. 4 However, under severe epidemic conditions, the disease can cause a total crop failure.…”

Section: Introductionmentioning

confidence: 99%

Antiviral activity of chitosan nanoparticles for controlling plant-infecting viruses

et al. 2022

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Chitosan nanoparticles (ChiNPs) are a potentially effective means for controlling numerous plant diseases. This study firstly describes the antiviral capabilities of ChiNPs to control plant viral diseases compared to its bulk form. Bean yellow mosaic virus (BYMV) was used as a model plant virus affecting faba bean plants and many other legumes. The antiviral effectiveness of ChiNPs and chitosan were evaluated as a curative application method, using six dosage rates (50, 100, 200, 250, 300 and 400 mg/L). Results indicated that ChiNPs curatively applied 48 h post virus inoculation entirely inhibit the disease infectivity and viral accumulation content at 300 mg/L and 400 mg/L. The virus titre was greatly alleviated within the plant tissues by 7.71% up to100% depending on ChiNP dosage rates. However, chitosan used in its bulk-based material form revealed a relatively low to an intermediate reduction in virus infectivity by 6.67% up to 48.86%. Interestingly, ChiNPs affect the virus particle’s integrity by producing defective and incomplete BYMV viral particles, defeating their replication and accumulation content within the plant tissues. Simultaneously, ChiNP applications were appreciably shown to promote the pathogenesis-related (PR-1) gene and other defence-related factors. The mRNA of the PR-1 gene was markedly accumulated in treated plants, reaching its maximum at 400 mg/L with 16.22-fold relative expression change over the untreated control. Further, the total phenol dynamic curve was remarkably promoted for 30 days in response to ChiNP application, as compared to the untreated control. Our results provide the first report that chitosan-based nanomaterials have a superior effect in controlling plant viruses as an antiviral curing agent, suggesting that they may feasibly be involved in viral disease management strategies under field conditions without serious health concerns and environmental costs.

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Identification and molecular characterization of Bean yellow mosaic virus infecting French bean in Himachal Pradesh

Cited by 18 publications

References 13 publications

Burdur İlinde Yetiştirilen Fasulye Bitkilerinde Bean Yellow Mosaic Virus (BYMV)’ünün Araştırılması

Burdur İlinde Yetiştirilen Fasulye Bitkilerinde Bean Yellow Mosaic Virus (BYMV)’ünün Araştırılması

Molecular Detection of Bean Yellow Mosaic Virus in Lupinus albus Plants and its Associated Alterations in Biochemical and Physiological Parameters

Antiviral activity of chitosan nanoparticles for controlling plant-infecting viruses

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