IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF TWO SERINE PROTEASES AND THEIR POTENTIAL INVOLVEMENT IN PROPHENOLOXIDASE ACTIVATION IN <i>Plutella xylostella</i>

Gao, Gang; Xu, Xi; Yu, Jing; Li, Linmiao; Ju, Wenyan; Jin, Fengliang; Freed, Shoaib

doi:10.1002/arch.21337

Cited by 5 publications

(2 citation statements)

References 33 publications

(38 reference statements)

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“…Serpin can prevent excessive melanism by inhibiting SPs/SPHs activity and blocking proPO activation. SPs/SPHs and Serpin rigorously regulate the activation of proPO in time and space, removing the invading exogenous substances and evading the detrimental effects of intermediates [42][43][44][45][46][47]. Therefore, the PO cascade is a good target for biological pest control.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Targets PAP1 to Mediate Melanization in Plutella xylostella (Linnaeus) Infected by Metarhizium anisopliae

Zhang,

Jin,

Huang

et al. 2024

IJMS

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MicroRNAs (miRNAs) play a pivotal role in important biological processes by regulating post-transcriptional gene expression and exhibit differential expression patterns during development, immune responses, and stress challenges. The diamondback moth causes significant economic damage to crops worldwide. Despite substantial advancements in understanding the molecular biology of this pest, our knowledge regarding the role of miRNAs in regulating key immunity-related genes remains limited. In this study, we leveraged whole transcriptome resequencing data from Plutella xylostella infected with Metarhizium anisopliae to identify specific miRNAs targeting the prophenoloxidase-activating protease1 (PAP1) gene and regulate phenoloxidase (PO) cascade during melanization. Seven miRNAs (pxy-miR-375-5p, pxy-miR-4448-3p, pxy-miR-279a-3p, pxy-miR-3286-3p, pxy-miR-965-5p, pxy-miR-8799-3p, and pxy-miR-14b-5p) were screened. Luciferase reporter assays confirmed that pxy-miR-279a-3p binds to the open reading frame (ORF) and pxy-miR-965-5p to the 3′ untranslated region (3′ UTR) of PAP1. Our experiments demonstrated that a pxy-miR-965-5p mimic significantly reduced PAP1 expression in P. xylostella larvae, suppressed PO activity, and increased larval mortality rate. Conversely, the injection of pxy-miR-965-5p inhibitor could increase PAP1 expression and PO activity while decreasing larval mortality rate. Furthermore, we identified four LncRNAs (MSTRG.32910.1, MSTRG.7100.1, MSTRG.6802.1, and MSTRG.22113.1) that potentially interact with pxy-miR-965-5p. Interference assays using antisense oligonucleotides (ASOs) revealed that silencing MSTRG.7100.1 and MSTRG.22113.1 increased the expression of pxy-miR-965-5p. These findings shed light on the potential role of pxy-miR-965-5p in the immune response of P. xylostella to M. anisopliae infection and provide a theoretical basis for biological control strategies targeting the immune system of this pest.

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Section: Discussionmentioning

confidence: 99%

MicroRNA Targets PAP1 to Mediate Melanization in Plutella xylostella (Linnaeus) Infected by Metarhizium anisopliae

Zhang,

Jin,

Huang

et al. 2024

IJMS

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“…Penicillium italicum (76) and humoral defense mechanism triggering in Plutella xylostella (148). Currently, many studies have been described proteases with serine and alkaline properties (44; 33; 149; 82; 150) due to stability in pH ranges, tolerance with severe conditions, as well as PMSF inhibition, which suggest the majority use of serine alkaline proteases in detergent formulations.…”

Section: Applicationsmentioning

confidence: 99%

Purificação e caracterização de protease de Aspergillus terreus VSP-22

Gimenes¹

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Ata de defesa com as respectivas assinaturas dos membros encontra-se no processo de vida acadêmica do aluno. DedicatóriaÀ Deus, pelo dom da vida e por ser meu amparo em todos os momentos, sejam de felicidade ou durante as batalhas do dia a dia. Sei que está sempre presente e ouve minhas orações.Aos meus pais, Marcos e Márcia, pelo amor e dedicação em minha educação. A vocês dedico este trabalho, por serem essência na minha formação pessoal e por transmitirem a garra de luta e vontade de vencer.Ao meu namorado Rafael, pelo carinho, companheirismo e apoio em mais uma etapa da minha vida. A quem esteve ao meu lado em todos os momentos difíceis e me deu suporte.Ao meu irmão Nathan, pela admiração e momentos de descontração.

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An entomopathogenic bacterium, Xenorhabdus hominickii ANU101, produces oxindole and suppresses host insect immune response by inhibiting eicosanoid biosynthesis

Sadekuzzaman

Park

Lee

et al. 2017

Journal of Invertebrate Pathology

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IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF TWO SERINE PROTEASES AND THEIR POTENTIAL INVOLVEMENT IN PROPHENOLOXIDASE ACTIVATION IN Plutella xylostella

Cited by 5 publications

References 33 publications

MicroRNA Targets PAP1 to Mediate Melanization in Plutella xylostella (Linnaeus) Infected by Metarhizium anisopliae

MicroRNA Targets PAP1 to Mediate Melanization in Plutella xylostella (Linnaeus) Infected by Metarhizium anisopliae

Purificação e caracterização de protease de Aspergillus terreus VSP-22

An entomopathogenic bacterium, Xenorhabdus hominickii ANU101, produces oxindole and suppresses host insect immune response by inhibiting eicosanoid biosynthesis

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