Identification and Molecular Characterizations of <i>Neoscytalidium dimidiatum</i> Causing Stem Canker of Red‐fleshed Dragon Fruit (<i>Hylocereus polyrhizus)</i> in Malaysia

Mohd, Masratul Hawa; Salleh, Baharuddin; Zakaria, Latiffah

doi:10.1111/jph.12146

Cited by 67 publications

(49 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Microscopic examination of the pathogen demonstrated branched and septate hyphae with no conidiophores. Consistent with [21], arthroconidia were thick-walled and barrel-shaped that could be found individually or in chains, ranging 5-15 x 3-6 µm in size ( Figure 2). Old cultures, of 25 days, developed hyaline pycnidial conidia when young, and dark brown central regions when aged.…”

Section: Discussionsupporting

confidence: 57%

“…Plant disease management mainly relies on the life cycle of the pathogen. N. dimidiatum produces two types of spores, pycniospores which are formed in pycnidia embedded in mature lesions and phragmospores which are formed by the breaking up of individual or groups of cells of mature hyphae in dead tissues of the lesion [20,21]. In culture, only phragmospores are formed and produced.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Characterization and Disease Control of Stem Canker on Royal Poinciana (Delonix regia) Caused by Neoscytalidium dimidiatum in the United Arab Emirates

Raish

Saeed

Sham

et al. 2020

IJMS

View full text Add to dashboard Cite

In the United Arab Emirates (UAE), royal poinciana (Delonix regia) trees suffer from stem canker disease. Symptoms of stem canker can be characterized by branch and leaf dryness, bark lesions, discoloration of xylem tissues, longitudinal wood necrosis and extensive gumming. General dieback signs were also observed leading to complete defoliation of leaves and ultimately death of trees in advanced stages. The fungus, Neoscytalidium dimidiatum DSM 109897, was consistently recovered from diseased royal poinciana tissues; this was confirmed by the molecular, structural and morphological studies. Phylogenetic analyses of the translation elongation factor 1-a (TEF1-α) of N. dimidiatum from the UAE with reference specimens of Botryosphaeriaceae family validated the identity of the pathogen. To manage the disease, the chemical fungicides, Protifert®, Cidely® Top and Amistrar® Top, significantly inhibited mycelial growth and reduced conidial numbers of N. dimidiatum in laboratory and greenhouse experiments. The described “apple bioassay” is an innovative approach that can be useful when performing fungicide treatment studies. Under field conditions, Cidely® Top proved to be the most effective fungicide against N. dimidiatum among all tested treatments. Our data suggest that the causal agent of stem canker disease on royal poinciana in the UAE is N. dimidiatum.

show abstract

Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Disease Control of Stem Canker on Royal Poinciana (Delonix regia) Caused by Neoscytalidium dimidiatum in the United Arab Emirates

Raish

Saeed

Sham

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Neoscytalidium dimidiatum is an opportunistic fungus of plants (Padin et al 2005). This pathogen can cause pitahaya canker and spot on the surface of the stem or fruit (Lan et al 2012;Chuang et al 2012;Yi et al 2013;Mohd et al 2013) and internal black rot in fruit (Ezra et al 2013). However, this is the first reported of internal browning in pitahaya in Guangdong Province, China.…”

mentioning

confidence: 98%

Fruit internal brown rot caused by Neoscytalidium dimidiatum on pitahaya in Guangdong province, China

Lin

et al. 2015

Australasian Plant Dis. Notes

View full text Add to dashboard Cite

In July 2013, an unknown fruit internal brown rot disease was observed on white-fleshed pitahaya (Hylocereus undatus) in Zhanjiang, Guangdong province, China. Based on morphological characteristics and phylogenetic analysis of ITS sequences, the pathogen was determined as Neoscytalidium dimidiatum.Keywords Hylocereus . Fungal diseases . Neoscytalidium dimidiatum Pitahaya (Hylocereus undatus), also known as strawberry pear or dragon fruit, is a cactaceous fruit crop cultivated widely in some tropical and subtropical countries because of its high nutrient content and numerous selling points such as the attractive shape, colour and the good internal properties for market (Le Bellec et al. 2006). In China, pitahaya is a great contributor to the regional farming economic development particularly in the Hainan, Guangdong, Fujian, Guangxi and Yunnan provinces; the cultivation area up to 13,000 ha on the mainland.In July 2013, an unknown fruit internal brown rot disease was observed on white-fleshed pitahaya in several orchards of Zhanjiang, Guangdong province. The disease caused the fruit to develop slower than uninfected fruit in the initial stage, induced fruit internal brown rot and made the peel colour change from green to red 10-15 days in advance of the normal fruit peel which turned red 24-25 days after anthesis (Nerd et al. 1999). The brown rot first occurred below the persistent style ( Fig. 1a) which gradually spread and developed into a rotten area. The conspicuous grey-white mycelia developed after the diseased fruit was cut in half lengthways and kept in a sealed plastic box for 3 days (Fig. 1b). The incidence of the disease was approximately 15 % in the field.Fruit with internal brown rot were collected from an orchard (21°8′52″N 110°18′28″E). After surface sterilisation with 75 % ethanol solution, a symptomatic piece of fruit tissue from the location of the brown rot was carefully extracted with sterile forceps and placed on potato dextrose agar (PDA) plates under aseptic conditions and then incubated at 25°C in the dark for 36 h. Hyphae growing from the tissue pieces were transferred and sub-cultured to obtain pure cultures. To obtain single spored isolates, conidia from pure cultures were suspended in sterile water and streaked onto PDA plates. The single-conidia forming colonies were obtained after incubation at 25°C in the dark for 24 h.Healthy fruit were used for pathogenicity tests in the laboratory and field. A suspension of approximately 10 4 spores/ mL was prepared from 4-day-old cultures grown at 25°C in the dark. Two hundred μl of the suspension was injected into the sarcocarp of healthy fruit with a sterile 32G hypodermic needle. Injection of isovolumetric sterile water served as a control. In the laboratory, all fruit were maintained in a sealed plastic box at 25°C in the dark. The symptoms of brown rot were observed on inoculated fruit 7 days postinoculation (Fig. 1c). Fruit in the laboratory rotted more quickly than in the field, whereas control fruit did not develop any symptoms ...

show abstract

“…A mycelial disc of 6 mm diameter was placed onto the center of PDA plate and incubated as above for 7 days. Water agar (WA) overlaid with toothpicks [18] was used to induce the formation of microsclerotia and pycnidia and incubated as above for 7 days. The size of 50 randomly selected conidia and the diameter of 50 microsclerotia and pycnidia were measured.…”

Section: Isolation and Morphological Identificationmentioning

confidence: 99%

Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia

et al. 2019

Self Cite

View full text Add to dashboard Cite

Crinum asiaticum and Hymenocallis littoralis, commonly known as spider lilies are bulbous perennial and herbaceous plants that widely planted in Malaysia as ornamental. During 2015-2016, symptom of leaf blight was noticed on the hosts from several locations in Penang. The symptom appeared as irregular brown to reddish lesions surrounded by yellow halos. As the disease progressed, the infected leaves became blighted, dried, and fell off with the presence of black microsclerotia and pycnidia on the lesions parts. The present study was conducted to investigate the causal pathogen of leaf blight on C. asiaticum and H. littoralis. Based on morphological characteristics and DNA sequences of internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF1-a) gene, the causal pathogen was identified as Macrophomina phaseolina. Phylogenetic analysis of combined dataset of ITS and TEF1-a grouped the isolates studied with other isolates of M. phaseolina from GenBank. The grouping of the isolates was supported by 96% bootstrap value. Pathogenicity test proved the role of the fungus in causing leaf blight on both hosts. ARTICLE HISTORY

show abstract

Identification and Molecular Characterizations of Neoscytalidium dimidiatum Causing Stem Canker of Red‐fleshed Dragon Fruit (Hylocereus polyrhizus) in Malaysia

Cited by 67 publications

References 26 publications

Molecular Characterization and Disease Control of Stem Canker on Royal Poinciana (Delonix regia) Caused by Neoscytalidium dimidiatum in the United Arab Emirates

Molecular Characterization and Disease Control of Stem Canker on Royal Poinciana (Delonix regia) Caused by Neoscytalidium dimidiatum in the United Arab Emirates

Fruit internal brown rot caused by Neoscytalidium dimidiatum on pitahaya in Guangdong province, China

Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia

Contact Info

Product

Resources

About