Identification and molecular profiling of a novel homolog of cystatin C from rock bream (Oplegnathus fasciatus) evidencing its transcriptional sensitivity to pathogen infections

Elvitigala, Don Anushka Sandaruwan; Lee, Jehee

doi:10.1007/s11033-021-06415-9

Cited by 5 publications

(2 citation statements)

References 36 publications

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“…The results show that the chitinases are present together with high levels of IgGFc‐binding‐like protein, cystatin, and fish‐egg lectin (Fig. 2 ), three proteins shown to play a potential role in the teleost immune system against pathogens [ 30 , 31 , 32 , 33 , 34 ]. Moreover, the Atlantic salmon stomach chitinases share many features with human AMCase [ 35 ]; they share the same conserved domains, they are abundant in the gastrointestinal tract, they are acid‐stable, and they are able to degrade crab shell chitin.…”

Section: Discussionmentioning

confidence: 99%

The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases

et al. 2023

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Atlantic salmon (Salmo salar) possesses a genome containing 10 genes encoding chitinases, yet their functional roles remain poorly understood. In other fish species, chitinases have been primarily linked to digestion, but also to other functions, as chitinase‐encoding genes are transcribed in a variety of non‐digestive organs. In this study, we investigated the properties of two chitinases belonging to the family 18 glycoside hydrolase (GH18) group, namely Chia.3 and Chia.4, both isolated from the stomach mucosa. Chia.3 and Chia.4, exhibiting 95% sequence identity, proved inseparable using conventional chromatographic methods, necessitating their purification as a chitinase pair. Detailed biochemical analysis revealed sustained chitinolytic activity against β‐chitin for up to 24 hours, spanning a pH range of 2 to 6. Moreover, subsequent in vitro investigations established that this chitinase pair efficiently degrades diverse chitin‐containing substrates into chitobiose (GlcNAc2), highlighting the potential of Atlantic salmon to utilize novel chitin‐containing feed sources. Analysis of the gastric matrix proteome demonstrate that the chitinases are secreted and rank among the most abundant proteins in the gastric matrix. This finding correlates well with the previously observed high transcription of the corresponding chitinase genes in Atlantic salmon stomach tissue. By shedding light on the secreted chitinases in the Atlantic salmon's stomach mucosa and elucidating their functional characteristics, this study enhances our understanding of chitinase biology in this species. Moreover, the observed capacity to effectively degrade chitin‐containing materials implies the potential utilization of alternative feed sources rich in chitin, offering promising prospects for sustainable aquaculture practices.

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Section: Discussionmentioning

confidence: 99%

The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases

et al. 2023

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“…In theory, chitinases could play a role in the digestion of chitin-containing feed and/or chitin-containing pathogens such as fungi. The results show that the chitinases are present together with high levels of IgGFc-binding-like protein, cystatin and fish-egg lectin (Figure 2), three proteins shown to play a potential role in the teleost immune system against pathogens [26][27][28][29][30] . Moreover, the Atlantic salmon stomach chitinases share many features with human AMCase 31 ; they share the same conserved domains, they are abundant in the gastrointestinal tract, they are acid-stable, and they are able to degrade crab shell chitin.…”

Section: Discussionmentioning

confidence: 99%

The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases

Holen

Tuveng

Kent

et al. 2022

Preprint

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The Atlantic salmon (Salmo salar) genome contains 10 chitinase encoding genes, but little is known about the function of these chitinases. We show that the protein products of three genes, the family 18 glycoside hydrolase (GH18) chitinases Chia.3, Chia.4, and Chia.7 are secreted in the stomach mucosa and are amongst the most abundant proteins in this matrix. Chia.3 and Chia.4, sharing 95% sequence identity, were not possible to separate by standard chromatographic methods and were thus purified as a chitinase pair. Biochemical analysis revealed chitinolytic activity towards β-chitin for up to 24 hours at pH 2-6. Further in vitro analysis showed that this chitinase pair efficiently degraded various chitin-containing substrates to chitobiose (GlcNAc2) suggesting that Atlantic salmon has the potential to utilize novel chitin-containing feed sources.

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Molecular characterization, expression and immune functional analysis of cystatin 10 in turbot

Zhang,

Duan,

Chen

et al. 2024

Mol Biol Rep

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Identification and molecular profiling of a novel homolog of cystatin C from rock bream (Oplegnathus fasciatus) evidencing its transcriptional sensitivity to pathogen infections

Cited by 5 publications

References 36 publications

The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases

The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases

The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases

Molecular characterization, expression and immune functional analysis of cystatin 10 in turbot

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