Identification and nucleotide sequence of the Leptospira biflexa serovar patoc trpE and trpG genes

Nucleotide sequence and analysis of a gene encoding anthranilate synthase component I in Spirochaeta aurantia

“…The apparent lack of trpG in the cloned trpE-containing DNA supports our previous hypothesis that the S. aurantia ASI polypeptide can interact with the E. coli ASII polypeptide to form an AS active with either glutamine or NH3 as the amino donor (1). The fact that the S. aurantia trpE gene is not closely linked to a trpG gene differs from the arrangement found in the L. biflexa chromosome, in which trpE and trpG are separated by 64 bp (31). This difference is not surprising considering the great evolutionary divergence between S. aurantia and L. biflexa (24).…”

“…In part because there has not yet been a genetic transfer system described for any spirochete, little is known about gene organization in these bacteria. However, several spirochete genes have been cloned and sequenced (for example, see references 1,3,19,23, and 29 to 31), and the chromosome of one of the spirochete species, Borrelia burgdorferi, is so far unique among procaryotes in that it is linear rather than circular (13).…”

“…The trpE gene and the adjacent trpG gene, which encode components I and II, respectively, of anthranilate synthase (AS), the first enzyme in the tryptophan biosynthetic pathway, were cloned from Leptospira biflexa (29,30) and have been sequenced (31). To begin our analysis of the molecular genetics of the SpirochaetaTreponema-Borrelia cluster, we cloned Spirochaeta aurantia DNA that complemented an Escherichia coli trpE deletion (1).…”

Amplification of trpEG: adaptation of Buchnera aphidicola to an endosymbiotic association with aphids.

“…An arrangement in which trpEG is unlinked to other trp genes is found in Rhizobium melioti (24), Thermus thermophilus (25), and Leptospira biflexa (45). In R. meliloti trpE(G) genes are fused, and enzyme activity is regulated by attenuation as is indicated by DNA encoding a tryptophancontaining leader peptide preceding trpE(G) and by mutational analysis (24,46).…”

Nucleotide sequence analysis of the Leptospira biflexa serovar patoc rpsL and rpsG genes

“…To circumvent these difficulties, we have used molecular cloning techniques to analyze Leptospira genes (32,34). The results of those and other studies suggest that Leptospira genes may be organized differently from homologous genes in other bacteria (7,25,33,34). In this report we present an analysis of the nucleotide sequences of two L. biflexa genes which exhibit significant sequence similarity to the Escherichia coli ribosomal protein genes rpsL (encoding ribosomal protein S12) and rpsG (encoding ribosomal protein S7).…”