Identification and Optimization of Indole-3-Acetic Acid Production of Endophytic Bacteria and Their Effects on Plant Growth

Khianngam, Saowapar; Meetum, Pimjai; Chiangmai, Pantipa Na; Tanasupawat, Somboon

doi:10.21315/tlsr2023.34.1.12

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…The highest concentration of IAA was found in the Pseudomonas putida D7 strain (Table 1). The amount of produced IAA varied between 45.2 and 69.2 µg mL −1 depending on the strain, which is similar to or significantly higher than that observed in other endophytic bacterial strains [30,31].…”

Section: Characterization Of the Biological Activity Of Endophytic Ba...supporting

confidence: 71%

Biopolymers as Seed-Coating Agent to Enhance Microbially Induced Tolerance of Barley to Phytopathogens

Usmanova,

Brazhnikova,

Omirbekova

et al. 2024

Polymers

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Infections of agricultural crops caused by pathogen ic fungi are among the most widespread and harmful, as they not only reduce the quantity of the harvest but also significantly deteriorate its quality. This study aims to develop unique seed-coating formulations incorporating biopolymers (polyhydroxyalkanoate and pullulan) and beneficial microorganisms for plant protection against phytopathogens. A microbial association of biocompatible endophytic bacteria has been created, including Pseudomonas flavescens D5, Bacillus aerophilus A2, Serratia proteamaculans B5, and Pseudomonas putida D7. These strains exhibited agronomically valuable properties: synthesis of the phytohormone IAA (from 45.2 to 69.2 µg mL−1), antagonistic activity against Fusarium oxysporum and Fusarium solani (growth inhibition zones from 1.8 to 3.0 cm), halotolerance (5–15% NaCl), and PHA production (2.77–4.54 g L−1). A pullulan synthesized by Aureobasidium pullulans C7 showed a low viscosity rate (from 395 Pa·s to 598 Pa·s) depending on the concentration of polysaccharide solutions. Therefore, at 8.0%, w/v concentration, viscosity virtually remained unchanged with increasing shear rate, indicating that it exhibits Newtonian flow behavior. The effectiveness of various antifungal seed coating formulations has been demonstrated to enhance the tolerance of barley plants to phytopathogens.

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Section: Characterization Of the Biological Activity Of Endophytic Ba...supporting

confidence: 71%

Biopolymers as Seed-Coating Agent to Enhance Microbially Induced Tolerance of Barley to Phytopathogens

Usmanova,

Brazhnikova,

Omirbekova

et al. 2024

Polymers

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“…In this study, growth media for legume plant root rhizosphere bacteria was added with 0.1 mg/l L-tryptophan to provide precursor material for the formation of IAA so that the resulting concentration of IAA is more optimal [29] because it is known that most bacteria are capable of producing IAA. from tryptophan [30]. L-tryptophan was a precursor that affected IAA production.…”

Section: Resultsmentioning

confidence: 99%

The ability of bacteria from legume plant roots grown on former coal mining soil to produce Indole-3-Acetic Acid (IAA)

Rahayu,

Yuliani,

Asri

2024

E3S Web Conf.

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In general, coal mining is carried out openly using heavy equipment to take and move soil in the topsoil area until coal mining is possible to be conducted. As a consequence, the nutrient level is low because there is physical, chemical, and biological soil damage. Bioremediation is one of the alternatives to improve former coal mining land by utilizing soil microorganisms that have a role in soil plant hormone levels, such as auxin-produced root rhizosphere bacteria. This study aimed to isolate and characterize rhizosphere bacteria of legume plant roots grown on former coal mining soil, and to determine qualitatively and quantitatively its ability to produce IAA hormones. The characterizations include gram properties, colony morphology, arrangement of isolate, and cell shape. Then, the bacterial ability to produce IAA qualitatively and quantitatively respectively using the Salkowski method and spectrophotometry were tested. The results revealed that there were eleven isolates of legume plant root rhizosphere bacteria grown on the former coal mining soil that were able to produce IAA hormones with an average concentration of 15.949 ppm (2IA4); 10.762 ppm (4IIE3); 9.700 ppm (ID3); 9.422 ppm (3IB4); 7.970 ppm (2IA3); 7.847 ppm (6IIB3); 7.268 ppm (8IIIB4); 6.804 ppm (IIID5); 6.459 ppm (IE5); 5.379 ppm (7IIIB3); and 5.086 ppm (5IB3). Isolates of rhizosphere bacteria with the highest concentration have the potential to be chosen as a growth booster for legume plants grown on former coal mining soil to increase legume crop productivity.

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Identification and genomic analysis of a thermophilic bacterial strain that reduces ammonia loss from composting

Chen,

Feng,

et al. 2024

Microbiol Spectr

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Ammonia loss is the most severe during the high-temperature stage (>50°C) of aerobic composting. Regulating ammonia volatilization during this period via thermophilic microbes can significantly improve the nitrogen content of compost and reduce air pollution due to ammonia loss. In this study, an ammonia-assimilating bacterial strain named LL-8 was screened out as having the strongest ammonia nitrogen conversion rate (32.7%) at high temperatures (50°C); it is able to significantly reduce 42.9% ammonia volatile loss in chicken manure composting when applied at a high-temperature stage. Phylogenetic analysis revealed that LL-8 was highly similar (>98%) with Priestia aryabhattai B8W22 T and identified as Priestia aryabhatta . Genomic analyses indicated that the complete genome of LL-8 comprised 5,060,316 base pairs with a GC content of 32.7% and encoded 5,346 genes. Genes, such as gudB , rocG , glnA , gltA , and gltB , that enable bacteria to assimilate ammonium nitrogen were annotated in the LL-8 genome based on the comparison to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The results implied that the application of thermophilic ammonia-assimilating strain P. aryabhatta LL-8 would be a promising solution to reduce ammonia loss and mitigate air pollution of aerobic composting. IMPORTANCE Aerobic composting is one of the essential ways to recycle organic waste, but its ammonia volatilization is severe and results in significant nitrogen loss, especially during the high-temperature period, which is also harmful to the environment. The application of thermophilic bacteria that can use ammonia as a nitrogen source at high temperatures is helpful to reduce the ammonia volatilization loss of composting. In this study, we screened and identified a bacteria strain called LL-8 with high temperature (50°C) resistance and strong ammonia-assimilating ability. It also revealed significant effects on decreasing ammonia volatile loss in composting. The whole-genome analysis revealed that LL-8 could utilize ammonium nitrogen by assimilation to decrease ammonia volatilization. Our work provides a theoretical basis for the application of this functional bacteria in aerobic composting to control nitrogen loss from ammonia volatilization.

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Identification and Optimization of Indole-3-Acetic Acid Production of Endophytic Bacteria and Their Effects on Plant Growth

Cited by 3 publications

References 46 publications

Biopolymers as Seed-Coating Agent to Enhance Microbially Induced Tolerance of Barley to Phytopathogens

Biopolymers as Seed-Coating Agent to Enhance Microbially Induced Tolerance of Barley to Phytopathogens

The ability of bacteria from legume plant roots grown on former coal mining soil to produce Indole-3-Acetic Acid (IAA)

Identification and genomic analysis of a thermophilic bacterial strain that reduces ammonia loss from composting

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