Identification and partial characterization of Rhizopus nigricans Gβ proteins and their expression in the presence of progesterone

Jeraj, Natasa; Stilla, Annalisa; Petrič, Špela; Girolamo, Maria Di; Črešnar, Bronislava; Lenasi, Helena

doi:10.1016/j.jsbmb.2010.12.015

Cited by 2 publications

(3 citation statements)

References 47 publications

(58 reference statements)

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“…In this study, we collected ovaries from the D280 and D580 hens and compared the follicle arrangement from large to small according to their diameter. We found that the number of PHFs in D580 hens was significantly reduced as compared with that in D280 hens, which was consistent with a previous study [28]. IF staining showed that FSHR was located in GCs of the SWFs, which was consistent with a previous report [1].…”

supporting

confidence: 93%

The Attenuating Effect of the Intraovarian Bone Morphogenetic Protein 4 on Age-Related Endoplasmic Reticulum Stress in Chicken Follicular Cells

Yao

Lin

et al. 2020

Oxidative Medicine and Cellular Longevity

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In the poultry, only less than 5% primordial follicles in the ovary can develop into the prehierarchical follicles (PHFs) leading to progressive development, ovulation, and egg formation. This low rate of recruitment indicates a huge potential for improvement of the laying performance. A great reduction in egg production is caused by aging with extensive follicular atresia. In this study, age-related changes in the laying performance and ovarian status were compared between the peak-lay (D280) and aged (D580) chickens. Subsequently, a cross coculture of PHFs and granulosa cells (GCs) from D280 or D580 hens was adopted to reveal the mechanism of declined follicle development. Results showed that persistent endoplasmic reticulum (ER) stress in GCs of the aged hens was accompanied with intensified apoptosis. Bone morphogenetic protein 4 (BMP4) secreted by GCs of PHFs in D280 hens was capable of relieving ER stress and improving follicular dominance for selection in D580 hens. During this action, BMP4 reduced free calreticulin (CALR, an ER marker) content and attenuated cell apoptosis in PHFs of D580 hens via the PERK-CHOP-BCL2/caspase3 or CALR-Ca2+-BCL2-caspase12 pathway. Furthermore, BMP4 prevented follicular atresia by promoting production of steroid hormones to improve survival of GCs in PHFs from the aged hens. In conclusion, intensified ER stress and apoptosis occurred in GCs of PHFs in aged chickens, while BMP4 secreted by GCs was capable of improving follicular viability by alleviating ER stress to promote follicular development.

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supporting

confidence: 93%

The Attenuating Effect of the Intraovarian Bone Morphogenetic Protein 4 on Age-Related Endoplasmic Reticulum Stress in Chicken Follicular Cells

Yao

Lin

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…Many studies have predicted the existence of a progesterone receptor in the membrane of filamentous fungi such as Rhizopus nigricans [27-30] but the molecular basis of steroid signalling in fungi remains unresolved [43,44]. Progesterone has been reported to bind to enriched plasma membrane fractions of R. nigricans with high affinity and this hormone has been reported to induce an activation of G proteins that decreases in the presence of cholera toxin [29].…”

Section: Discussionmentioning

confidence: 99%

“…Progesterone has been reported to bind to fungal membranes but the direct identification of specific progesterone receptors has not been reported until now. In Rhizopus , membrane ligand-binding assays suggest the presence of a progesterone receptor but that has not led to the identification of the specific receptor [27-30]. …”

Section: Introductionmentioning

confidence: 99%

Characterization and ligand identification of a membrane progesterone receptor in fungi: existence of a novel PAQR in Sporothrix schenckii

2012

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BackgroundAdaptive responses in fungi result from the interaction of membrane receptors and extracellular ligands. Many different classes of receptors have been described in eukaryotic cells. Recently a new family of receptors classified as belonging to the progesterone-adiponectin receptor (PAQR) family has been identified. These receptors have the seven transmembrane domains characteristic of G-protein coupled receptors, but their activity has not been associated directly to G proteins. They share sequence similarity to the eubacterial hemolysin III proteins.ResultsA new receptor, SsPAQR1 (Sporothrixschenckiiprogesterone-adiponectinQ receptor1), was identified as interacting with Sporothrix schenckii G protein alpha subunit SSG-2 in a yeast two-hybrid assay. The receptor was identified as a member of the PAQR family. The cDNA sequence revealed a predicted ORF of 1542 bp encoding a 514 amino acids protein with a calculated molecular weight of 57.8 kDa. Protein domain analysis of SsPAQR1 showed the 7 transmembrane domains (TM) characteristic of G protein coupled receptors and the presence of the distinctive motifs that characterize PAQRs. A yeast-based assay specific for PAQRs identified progesterone as the agonist. S. schenckii yeast cells exposed to progesterone (0.50 mM) showed an increase in intracellular levels of 3′, 5′ cyclic adenosine monophosphate (cAMP) within the first min of incubation with the hormone. Different progesterone concentrations were tested for their effect on the growth of the fungus. Cultures incubated at 35°C did not grow at concentrations of progesterone of 0.05 mM or higher. Cultures incubated at 25°C grew at all concentrations tested (0.01 mM-0.50 mM) with growth decreasing gradually with the increase in progesterone concentration.ConclusionThis work describes a receptor associated with a G protein alpha subunit in S. schenckii belonging to the PAQR family. Progesterone was identified as the ligand. Exposure to progesterone increased the levels of cAMP in fungal yeast cells within the first min of incubation suggesting the connection of this receptor to the cAMP signalling pathway. Progesterone inhibited the growth of both the yeast and mycelium forms of the fungus, with the yeast form being the most affected by the hormone.

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Identification and partial characterization of Rhizopus nigricans Gβ proteins and their expression in the presence of progesterone

Cited by 2 publications

References 47 publications

The Attenuating Effect of the Intraovarian Bone Morphogenetic Protein 4 on Age-Related Endoplasmic Reticulum Stress in Chicken Follicular Cells

The Attenuating Effect of the Intraovarian Bone Morphogenetic Protein 4 on Age-Related Endoplasmic Reticulum Stress in Chicken Follicular Cells

Characterization and ligand identification of a membrane progesterone receptor in fungi: existence of a novel PAQR in Sporothrix schenckii

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