Identification and partial characterization of Taastrup virus: a newly identified member species of the Mononegavirales

Böck, Julia; Lundsgaard, T.; Pedersen, Per Amstrup; Christensen, Laurids Siig

doi:10.1016/j.virol.2003.10.017

Cited by 12 publications

(14 citation statements)

References 48 publications

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“…Similar genome organization, N-P-M-G-U1-L, and conserved transcription motifs have recently been reported in two mosquito rhabdoviruses, Arboretum virus (ABTV) and Puerto Almendras virus (PTAMV)[18]. A limited region of the SfRV L protein showed similarity to domain III of rhabdovirus RNA-dependent RNA-polymerase and corresponding region in the Taastrup virus, a plant rhabdovirus transmitted by a leafhopper[19]. Also, a search for SfRV RNA sequences in the public sequence database indicated a high similarity of the SfRV M gene to a mRNA clone derived from BmN cells (AK377209.1).…”

Section: Introductionmentioning

confidence: 65%

Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line

Hashimoto¹,

Macri²,

Srivastava³

et al. 2017

PLoS ONE

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A putative novel rhabdovirus (SfRV) was previously identified in a Spodoptera frugiperda cell line (Sf9 cells [ATCC CRL-1711 lot 58078522]) by next generation sequencing and extensive bioinformatic analysis. We performed an extensive analysis of our Sf9 cell bank (ATCC CRL-1711 lot 5814 [Sf9L5814]) to determine whether this virus was already present in cells obtained from ATCC in 1987. Inverse PCR of DNA isolated from Sf9 L5814 cellular DNA revealed integration of SfRV sequences in the cellular genome. RT-PCR of total RNA showed a deletion of 320 nucleotides in the SfRV RNA that includes the transcriptional motifs for genes X and L. Concentrated cell culture supernatant was analyzed by sucrose density gradient centrifugation and revealed a single band at a density of 1.14 g/ml. This fraction was further analysed by electron microscopy and showed amorphous and particulate debris that did not resemble a rhabdovirus in morphology or size. SDS-PAGE analysis confirmed that the protein composition did not contain the typical five rhabdovirus structural proteins and LC-MS/MS analysis revealed primarily of exosomal marker proteins, the SfRV N protein, and truncated forms of SfRV N, P, and G proteins. The SfRV L gene fragment RNA sequence was recovered from the supernatant after ultracentrifugation of the 1.14 g/ml fraction treated with diethyl ether suggesting that the SfRV L gene fragment sequence is not associated with a diethyl ether resistant nucleocapsid. Interestingly, the 1.14 g/ml fraction was able to transfer baculovirus DNA into Sf9L5814 cells, consistent with the presence of functional exosomes. Our results demonstrate the absence of viral particles in ATCC CRL-1711 lot 5814 Sf9 cells in contrast to a previous study that suggested the presence of infectious rhabdoviral particles in Sf9 cells from a different lot. This study highlights how cell lines with different lineages may present different virosomes and therefore no general conclusions can be drawn across Sf9 cells from different laboratories.

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Section: Introductionmentioning

confidence: 65%

Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line

Hashimoto¹,

Macri²,

Srivastava³

et al. 2017

PLoS ONE

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“…Phylogenetic analysis also indicated that Sf-rhabdovirus and Taastrup virus branched together, but their relatedness to other viruses could not be established because the sequence diversity resulted in low bootstrap values (Ͻ70). However, Sf-rhabdovirus was distinct from Taastrup virus, since it was not related to any known virus in any other gene except in a limited region of the L-protein gene, whereas the N protein of Taastrup virus was related to the N proteins of several members of the Mononegavirales (25). Furthermore, Sf-rhabdovirus was distinct from plant rhabdoviruses, since it had only one additional ORF, which was located between the Gand L-protein genes, instead of several additional ORFs, with one of those being between the P-and M-protein genes (37).…”

Section: Discussionmentioning

confidence: 99%

“…Over 200 rhabdoviruses have been identified (31), and a majority of the plant rhabdoviruses and some vertebrate rhabdoviruses use an insect vector for transmission. However, only a few rhabdoviruses have been directly isolated from insects or insect cell lines; these belong to the order Diptera (such as sigma viruses in Drosophila and Muscina flies [26,32,33] and the Culex tritaeniorhynchus rhabdovirus [CTRV] in Culex mosquitoes [34]) and to the order Hemiptera (such as Taastrup virus in the leafhopper [25]). We report here the discovery of a novel rhabdovirus in the Spodoptera frugiperda Sf9 cell line, which is the first rhabdovirus found in the order Lepidoptera.…”

Section: Discussionmentioning

confidence: 99%

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

Galvin

Glasner

et al. 2014

J Virol

127

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The Sf9 cell line, derived from Spodoptera frugiperda, is used as a cell substrate for biological products, and no viruses have been reported in this cell line after extensive testing. We used degenerate PCR assays and massively parallel sequencing ( IMPORTANCEThe Spodoptera frugiperda Sf9 cell line is used as a cell substrate for the development and manufacture of biological products. Extensive testing has not previously identified any viruses in this cell line. This paper reports on the identification and characterization of a novel rhabdovirus in Sf9 cells. This was accomplished through the use of next-generation sequencing platforms, de novo assembly tools, and extensive bioinformatics analysis. Rhabdovirus identification was further confirmed by transmission electron microscopy. Infectivity studies showed the lack of replication of Sf-rhabdovirus in human cell lines. The overall study highlights the use of a combinatorial testing approach including conventional methods and new technologies for evaluation of cell lines for unexpected viruses and use of comprehensive bioinformatics strategies for obtaining confident next-generation sequencing results.

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“…The glycoproteins of rhabdoviruses, filoviruses, and bornaviruses are classical type I transmembrane proteins containing a cleavable signal sequence and an exterior N terminus and cytoplasmic C terminus, while the glycoproteins of paramyxoviruses are type II transmembrane proteins with a cytoplasmic N terminus, an exterior C terminus, and a noncleavable signal peptide (7). Using SignalP 3.0 (5) and PolyPhobius (15), we looked for the presence and cleavability of a signal peptide and the presence and orientation of transmembrane regions in the NYMV and MIDWV ORF proteins.…”

Section: Observationmentioning

confidence: 99%

Nyamanini and Midway Viruses Define a Novel Taxon of RNA Viruses in the Order Mononegavirales

Mihindukulasuriya

Nguyen

et al. 2009

J Virol

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Here, we report the sequencing and classification of Nyamanini virus (NYMV) and Midway virus (MIDWV), two antigenically related viruses that were first isolated in 1957 and 1966, respectively. Although these viruses have been cultured multiple times from cattle egrets, seabirds, and their ticks, efforts to classify them taxonomically using conventional serological and electron microscopic approaches have failed completely. We used a random shotgun sequencing strategy to define the genomes of NYMV and MIDWV. Contigs of 11,631 and 11,752 nucleotides, representing the complete genome of NYMV and the near-complete genome of MIDWV, respectively, were assembled. Each virus genome was predicted to carry six open reading frames (ORFs). BLAST analysis indicated that only two of the ORF proteins of each virus, the putative nucleocapsid and polymerase, had detectable sequence similarity to known viral proteins. Phylogenetic analysis of these ORF proteins demonstrated that the closest relatives of NYNV and MIDWV are negative-stranded-RNA viruses in the order Mononegavirales. On the basis of their very limited sequence similarity to known viruses, we propose that NYMV and MIDWV define a novel genus, Nyavirus, in this order.Nyamanini virus (NYMV) was first isolated in 1957 from a cattle egret (Bubulcus ibis) in South Africa (24). It has subsequently been isolated in Nigeria, Egypt, India, and Thailand from cattle egrets and Argas walkerae ticks (14,16,24). Although there has been no recognized human infection or disease associated with NYMV, suckling mice succumbed to NYMV infection 7 or 8 days after intracerebral inoculation (14). NYMV has not been definitively characterized or classified to date (10).Midway virus (MIDWV) was first isolated in 1966 from seabird ticks of two species [Ornithodoros (Alectorobius) spp.] collected on the Midway, Kure, and Manana islands in the Central Pacific and from northern Honshu, Japan. In addition, on Aomatsushima Island, nestling seabirds of two seabird species, Larus crassirostris and Nycticorax nycticorax, were found to have antibody to MIDWV. This virus is pathogenic for newborn Swiss mice but not 4-week-old Swiss mice injected intracranially. Also, the virus is cytopathic in BHK-21 cells and produces plaques in Vero cells. Efforts to classify MIDWV have revealed only that MIDWV is antigenically related to NYMV in cross-box complement fixation (CF) assays (22).To date, conventional approaches, such as serological analysis and electron microscopy (EM), have not yielded definitive characterization of MIDWV or NYMV. Heretofore, it was not known whether MIDWV and NYMV are DNA viruses or RNA viruses or to which virus family they belong. In this paper, we describe the application of unbiased high-throughput sequencing to define the genome sequences of NYMV and MIDWV. By analysis of their genomes, NYMV and MIDWV were determined to be negative-stranded RNA viruses highly divergent from all known viruses but most closely related to viruses in the order Mononegavirales. On the basis of the analys...

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Identification and partial characterization of Taastrup virus: a newly identified member species of the Mononegavirales

Cited by 12 publications

References 48 publications

Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line

Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

Nyamanini and Midway Viruses Define a Novel Taxon of RNA Viruses in the Order Mononegavirales

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