Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico

Mendoza-Rodriguez, Cynthia; Ocampo‐Candiani, Jorge; Claudio, Pilar Morales-San; Vazquez-Martínez, Osvaldo; Salinas-Santander, Mauricio; Torres‐López, Ernesto

doi:10.11648/j.ijcems.20200605.12

Cited by 1 publication

(2 citation statements)

References 15 publications

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“…It is to be noted that the samples were collected from these patients when they visited the clinic reporting overt HSV lesions in the labial/genital sites. Recently similar incidents have also been reported from Mexico where clinical HSV isolates from immunocompromised patients were found positive for tk-PCR while one remained negative in in vitro culture 32 . This non-CPE feature is unusual for HSV.…”

Section: Discussionsupporting

confidence: 69%

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Non-cytopathic herpes simplex virus type-1 isolated from acyclovir-treated patients with recurrent infections

Roy

Sukla

et al. 2022

Sci Rep

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Herpes simplex virus (HSV) usually produces cytopathic effect (CPE) within 24-72 h post-infection (P.I.). Clinical isolates from recurrent HSV infections in patients on Acyclovir therapy were collected between 2016 and 2019 and tested in cell cultures for cytopathic effects and further in-depth characterization. Fourteen such isolates did not show any CPE in A549 or Vero cell lines even at 120 h P.I. However, these cultures remained positive for HSV-DNA after several passages. Sequence analysis revealed that the non-CPE isolates were all HSV-1. Analysis of the thymidine kinase gene from the isolates revealed several previously reported and two novel ACV-resistant mutations. Immunofluorescence and Western blot data revealed a low-level expression of the immediate early protein, ICP4. Late proteins like ICP5 or capsid protein, VP16 were almost undetectable in these isolates. AFM imaging revealed that the non-CPE viruses had structural deformities compared to wild-type HSV-1. Our findings suggest that these strains are manifesting an unusual phenomenon of being non-CPE herpesviruses with low level of virus protein expressions over several passages. Probably these HSV-1 isolates are evolving towards a more “cryptic” form to establish chronic infection in the host thereby unraveling yet another strategy of herpesviruses to evade the host immune system.

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Section: Discussionsupporting

confidence: 69%

“…Other than a recent report from Mexico 32 , we know of no other precedence of these unusual forms of non-CPE clinical strains of HSV-1. The striking fact is that although the clinical isolates were non-CPE, the patients repeatedly presented with clinical manifestation of HSV infection in the form of oral or genital lesions.…”

Section: Discussionmentioning

confidence: 81%

Non-cytopathic herpes simplex virus type-1 isolated from acyclovir-treated patients with recurrent infections

Roy

Sukla

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico

Cited by 1 publication

References 15 publications

Non-cytopathic herpes simplex virus type-1 isolated from acyclovir-treated patients with recurrent infections

Non-cytopathic herpes simplex virus type-1 isolated from acyclovir-treated patients with recurrent infections

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