Identification and pathogenicity of<i>Botryosphaeria</i>species associated with grapevine decline in Western Australia

Taylor, Ashby B.; Hardy, G.; Wood, PM; Burgess, Treena I.

doi:10.1071/ap05018

Cited by 134 publications

(144 citation statements)

References 21 publications

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“…The relatively common occurrence of L. theobromae in Cameroon, compared to the other regions sampled in this study, could also reflect a climatic influence. Lasiodiplodia theobromae appears to occur most commonly in consistently warm areas (Taylor et al 2005;Urbez-Torrez et al 2008) and the climatic conditions in the localities where samples were collected in this study apparently support the findings.…”

Section: Fig 9 Mean Cambial Lesion Lengths (Mm) For Each Botryosphasupporting

confidence: 81%

Botryosphaeriaceae associated with Terminalia catappa in Cameroon, South Africa and Madagascar

et al. 2009

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Species in the Botryosphaeriaceae represent some of the most important fungal pathogens of woody plants. Although these fungi have been relatively well studied on economically important crops, hardly anything is known regarding their taxonomy or ecology on native or non-commercial tree species. The aim of this study was to compare the diversity and distribution of the Botryosphaeriaceae on Terminalia catappa, a tropical tree of Asian origin planted as an ornamental in Cameroon, Madagascar and South Africa. A total of 83 trees were sampled, yielding 79 Botryosphaeriaceae isolates. Isolates were initially grouped based on morphology of cultures and conidia. Representatives of the different morphological groups were then further characterised using sequence data for the ITS, tef 1-alpha, rpb2, BOTF15 and beta-tubulin gene regions. Five species of the Botryosphaeriaceae were identified, including Neofusicoccum parvum, N. batangarum sp. nov., Lasiodiplodia pseudotheobromae, L. theobromae and L. mahajangana sp. nov. Lasiodiplodia pseudotheobromae and L. theobromae, were the most commonly isolated species (62%), and were found at all the sites. Neofusicoccum parvum and N. batangarum were found in South Africa and Cameroon, respectively, whereas L. mahajangana was found only in Madagascar. Greenhouse inoculation trials performed on young T. catappa trees showed variation among isolates tested, with L. pseudotheobromae being the most pathogenic. The Botryosphaeriaceae infecting T. catappa appear to be dominated by generalist species that also occur on various other hosts in tropical and sub-tropical climates.

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Section: Fig 9 Mean Cambial Lesion Lengths (Mm) For Each Botryosphasupporting

confidence: 81%

Botryosphaeriaceae associated with Terminalia catappa in Cameroon, South Africa and Madagascar

et al. 2009

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“…The mycelial mass was harvested and placed into 1.5 mL sterile Eppendorf tubes. A modified method from Raeder and Broda (1985) was used to extract the DNA (Taylor et al, 2005). DNA was purified using the Ultrabind DNA purification kit in accordance with the manufacturer's instructions (MO BIO Laboratories, Solana Beach, California).…”

Section: Dna Extraction Pcr Amplification and Sequencingmentioning

confidence: 99%

“…These primers amplified a 500 bp region of RPB2 for all isolates except MUCC211 for which primers RPB2bot6F and FrRPB2-7cR were used. PCR products were cleaned and sequenced as described previously (Taylor et al, 2005). …”

Section: Dna Extraction Pcr Amplification and Sequencingmentioning

confidence: 99%

Use of the Genealogical Sorting Index (GSI) to delineate species boundaries in the Neofusicoccum parvum–Neofusicoccum ribis species complex

Sakalidis

Hardy

Burgess

2011

Molecular Phylogenetics and Evolution

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“…Thus, their pathogenicity has been underestimated, and their epidemiology remains not fully investigated. To date, 30 botryosphaeriaceous species, including Neofusicoccum parvum, have been reported to be involved in dieback disease (Spagnolo et al, 2014a;Luque et al, 2009;Taylor et al, 2005;Úrbez-Torres, 2011). The wood symptoms of botryosphaeria dieback are associated with brown stripes located in the outer xylem.…”

Section: Introductionmentioning

confidence: 99%

Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria dieback of grapevine

et al. 2015

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Liquid chromatography-diode array screening of the organic extract of the cultures of 13 isolates of the fungus Neofusicoccum parvum, the main causal agent of botryosphaeria dieback of grapevine, showed similar metabolites. One strain was selected for further chemical studies and led to the isolation and characterisation of 13 metabolites. Structures were elucidated through spectroscopic analyses, including one-and two-dimensional NMR and mass spectrometry, and through comparison to literature data. The isolated compounds belong to four different chemical families: five metabolites, namely, (À)-terremutin (1), (+)-terremutin hydrate (2), (+)-epi-sphaeropsidone (3) (À)-4-chloro-terremutin hydrate (4) and(+)-4-hydroxysuccinate-terremutin hydrate (5), belong to the family of dihydrotoluquinones; two metabolites, namely, (6S,7R) asperlin (6) (11), belong to the family of dihydroisocoumarins; and two of the metabolites, namely, 6-methyl-salicylic acid (12) and 2-hydroxypropyl salicylic acid (13), belong to the family of hydroxybenzoic acids. We determined the phytotoxic activity of the isolated metabolites through a leaf disc assay and the expression of defence-related genes in Vitis vinifera cells cv. Chardonnay cultured with (À)-terremutin (1), the most abundant metabolite. Finally, analysis of the brown stripes of grapevine wood from plants showing botryosphaeria dieback symptoms revealed the presence of two of the isolated phytotoxins. and (6R,7S)-dia-asperlin (7), belong to the family of epoxylactones; four metabolites, namely, (R)-(À)-mellein (8), (3R,4R)-4-hydroxymellein (9), (3R,4S)-4-hydroxymellein (10) (R)(À)-3-hydroxymellein

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Identification and pathogenicity ofBotryosphaeriaspecies associated with grapevine decline in Western Australia

Cited by 134 publications

References 21 publications

Botryosphaeriaceae associated with Terminalia catappa in Cameroon, South Africa and Madagascar

Botryosphaeriaceae associated with Terminalia catappa in Cameroon, South Africa and Madagascar

Use of the Genealogical Sorting Index (GSI) to delineate species boundaries in the Neofusicoccum parvum–Neofusicoccum ribis species complex

Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria dieback of grapevine

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