Identification and phylogenetic relationship of Iranian strains of various Leishmania species isolated from cutaneous and visceral cases of leishmaniasis based on N-acetylglucosamine-1-phosphate transferase gene

Hajjaran, Homa; Mohebali, Mehdi; Teimouri, Aref; Oshaghi, Mohammad Ali; Mirjalali, Hamed; Kazemirad, Elham; Shiee, Mohammad Reza; Naddaf, Saied Reza

doi:10.1016/j.meegid.2014.05.026

Cited by 28 publications

(23 citation statements)

References 33 publications

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“…Strategies include histone 2B RFLP analysis (184); sequencing and RFLP analysis of N-acetylglucosamine-1-phosphate transferase (181,224); PCR and RFLP analysis of hydrophilic acylated surface protein B, also known as K26 (9,225); glycosomal glyceraldehyde 3-phosphate dehydrogenase sequencing (24); meta2 RFLP analysis (226); chitinase sequencing (27); sequence analysis of DNA and RNA polymerase genes (227); pteridine reductase 1 sequencing (228); ␤-tubulin analysis (229); heat shock protein 20 sequencing (230); and sequence analysis of a calmodulin intergenic spacer (231).…”

Section: Miscellaneous Targetsmentioning

confidence: 99%

Species Typing in Dermal Leishmaniasis

2015

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SUMMARY Leishmania is an infectious protozoan parasite related to African and American trypanosomes. All Leishmania species that are pathogenic to humans can cause dermal disease. When one is confronted with cutaneous leishmaniasis, identification of the causative species is relevant in both clinical and epidemiological studies, case management, and control. This review gives an overview of the currently existing and most used assays for species discrimination, with a critical appraisal of the limitations of each technique. The consensus taxonomy for the genus is outlined, including debatable species designations. Finally, a numerical literature analysis is presented that describes which methods are most used in various countries and regions in the world, and for which purposes.

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Section: Miscellaneous Targetsmentioning

confidence: 99%

Species Typing in Dermal Leishmaniasis

2015

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“…infantum [ 4 ]. Recent studies showed a high prevalence of CL in Iran [ 5 , 6 ], Turkey and Syria [ 7 ]. Although Iraq shares long borders with these countries and leishmaniasis is endemic, the World Health Organization has not classified it as a country with a high burden profile [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

An outbreak of Leishmania major from an endemic to a non-endemic region posed a public health threat in Iraq from 2014-2017: Epidemiological, molecular and phylogenetic studies

et al. 2018

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BackgroundCutaneous leishmaniasis (CL) is a neglected worldwide, zoonotic, vector-borne, tropical disease that is a threat to public health. This threat may spread from endemic to non-endemic areas. Current research has exploited epidemiological, molecular and phylogenetical studies to determine the danger of an outbreak of CL in the borderline area between northern and central Iraq from 2014–2017.Methodology/Principal findingsFor the first time, using sequence analysis of the cytochrome b gene, the occurrence of CL in the borderline area between northern and central Iraq was confirmed to be due to Leishmania major. The phylogenetic analysis indicated that it was closely related to the L. major MRHO/IR/75/ER strain in Iran.Conclusions and significanceIn conclusion, the genotype confirmation of the L. major strain will improve our understanding of the epidemiology of the disease. This is important for facilitating control programs to prevent the further spread of CL. Furthermore, this area could be considered as a model for further research on the risk of global CL epidemics in other non-endemic countries where both reservoir hosts and sandfly vectors are present.

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“…Reports from other parts of Iran also indicate that the main causative agent for CL is L. major in Semnan, Golestan, North Khorasan, Isfahan, Fars, Hormozgan, and Ilam provinces whereas the predominant species in Kerman, Yazd, and Razavi Khorasan provinces has been L. tropica (15). Sarkari (17). It seems that nagt gene is better than LACK gene to indicate diversity of Leishmania species in this area.…”

Section: Discussionmentioning

confidence: 89%

Evaluation of LACK Gene Diversity in Leishmania major Using PCR and Sequencing Methods

Kardooni

Rahdar

Teimoori

et al. 2017

Jundishapur J Microbiol

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Background: Cutaneous leishmaniasis is one of the most important tropical diseases in many parts of the world caused by two main species of parasites including Leishmania major and L. tropica in the old world. Several attempts have been made to reach an effective and reliable vaccine against the disease with a spectrum of success. Selection of an effective target for vaccination needs to recognize a protein antigen with minor diversity. Leishmania activated C kinase (LACK) antigen is one of the good targets for this purpose that needs to be evaluated for its diversity in local study areas.

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Identification and phylogenetic relationship of Iranian strains of various Leishmania species isolated from cutaneous and visceral cases of leishmaniasis based on N-acetylglucosamine-1-phosphate transferase gene

Cited by 28 publications

References 33 publications

Species Typing in Dermal Leishmaniasis

Species Typing in Dermal Leishmaniasis

An outbreak of Leishmania major from an endemic to a non-endemic region posed a public health threat in Iraq from 2014-2017: Epidemiological, molecular and phylogenetic studies

Evaluation of LACK Gene Diversity in Leishmania major Using PCR and Sequencing Methods

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