Identification and phylogenetic study of bioluminescent bacteria from squid (Loligo duvaucelii) based on 16S rRNA gene

Pertiwi, Melati Putri; Praseptin, Patricia Karin Himawan; Werdani, Ika Diana; Listyorini, Dwi; Prabaningtyas, Sitoresmi

doi:10.1063/1.5050134

Cited by 1 publication

(2 citation statements)

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“…Target gene amplification was carried out by PCR technique using universal primers for 16S rRNA gene. The forward primer used was 27F, 5'-AGAGTTTGATCMTGGCTCAG-3', and reverse primer used was R1492, 5'-TACGGYTACCTTGTTACGACT-3' (Pertiwi et al, 2018). Pre-denaturation was carried out at 95°C for 3 minutes, followed by 40 amplification cycles consisting of denaturation at 95°C for 1 minute, annealing at 50°C for 1 minute, extension at 72°C for 1 minute, and the final extension was carried out at 72°C for 10 minutes.…”

Section: Genetic Identification (Dna Barcoding)mentioning

confidence: 99%

“…Pre-denaturation was carried out at 95°C for 3 minutes, followed by 40 amplification cycles consisting of denaturation at 95°C for 1 minute, annealing at 50°C for 1 minute, extension at 72°C for 1 minute, and the final extension was carried out at 72°C for 10 minutes. Electrophoresis was performed to visualize the PCR product on 1% agarose gel running in 50 volts electrical current for 60 minutes (Pertiwi et al, 2018). The results of electrophoresis are visualized using a UV trans illuminator.…”

Section: Genetic Identification (Dna Barcoding)mentioning

confidence: 99%

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Genetic Identification of Superbugs from River Streams in State University of Malang

Sholekah

Listyorini

2023

JRBA

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Superbugs revert to bacterial strains, which exhibit resistance to antibiotics. These bacteria could cause some problems in disease treatment and the environment. This research aimed of this study was to identify the presence of superbugs in the river stream that flows through Malang State University campus area. Amoxicillin, tetracycline, and chloramphenicol were selected to test the possible bacterial resistance. Water samples taken from a decided spot were spread over Luria Bertani’s agar on both antibiotic supplemented and antibiotics-free media with no delay. Molecular identification was carried out using 16S rRNA gene DNA barcoding approach, completed with morphological and Gram staining analyses. A total of 16 isolates of gram-negative colonies were found in the form of bacilli, diplobacilli, cocci, and diplococci. The genetic identification of eight resistant colonies led us to suggest that the isolates may belong to Aeromonas, Shigella, and Bacillus. Further studies are still required to get a clearer view of the correct taxonomical position of those resistant isolates.

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Section: Genetic Identification (Dna Barcoding)mentioning

confidence: 99%

Section: Genetic Identification (Dna Barcoding)mentioning

confidence: 99%