Identification and physical mapping of genes expressed in the <i>corpus luteum</i> in cattle<sup>1</sup>

Bønsdorff, Tina Bjørnlund; Eggen, Andre A.; Gautier, Mathieu; Åsheim, H.-C.; Rønningen, K.; Lingaas, Frode; Olsaker, I.

doi:10.1046/j.1365-2052.2003.01023.x

Cited by 5 publications

(9 citation statements)

References 45 publications

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“…Approximately 2 × 10 5 cDNA clones were transferred to nylon membranes by colony lifts (Sambrook et al. 1989) and screened with the previously identified BTCL9 RDA fragment (Bønsdorff et al. 2003).…”

Section: Screening Of a Cdna And A Bac Library With Btcl9mentioning

confidence: 99%

“…2002), using the primers listed in Table 1. The mapping was performed and analysed as previously described (Bønsdorff et al. 2003).…”

Section: Rh Mappingmentioning

confidence: 99%

“…Tissue samples were stored in RNAlater (Ambion Europe Ltd, Huntingdon, UK) at −20 °C, until RNA extraction. Total RNA was extracted and first strand cDNA was produced as previously described (Bønsdorff et al. 2003).…”

Section: Expression Analysismentioning

confidence: 99%

“…1999). For cattle, the only report is our previous identification of a gene fragment ( Bos taurus corpus luteum, BTCL9) of PAICS found in a screen for genes expressed in corpus luteum (CL) (Bønsdorff et al. 2003).…”

Section: Introductionmentioning

confidence: 99%

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Mapping of the bovine genes of the de novo AMP synthesis pathway¹

et al. 2004

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Summary The purine nucleotides adenosine monophosphate (AMP) and guanosine monophosphate (GMP) are critical for energy metabolism, cell signalling and cell reproduction. Despite their essential function, little is known about the regulation and in vivo expression pattern of the genes involved in the de novo purine synthesis pathway. The complete coding region of the bovine phosphoribosylaminoimidazole carboxylase gene (PAICS), which catalyses steps 6 and 7 of the de novo purine biosynthesis pathway, as well as bovine genomic sequences of the six other genes in the pathway producing inosine monophosphate (IMP) and AMP [phosphoribosyl pyrophosphate amidotransferase (PPAT), phosphoribosylglycinamide formyltransferase (GART), phosphoribosylformylglycinamidine synthase (PFAS), adenylosuccinate lyase (ADSL), 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) and adenylosuccinate synthase (ADSS)], were identified. The genes were mapped to segments of six different bovine chromosomes using a radiation hybrid (RH) cell panel. The gene PPAT, coding for the presumed rate-limiting enzyme of the purine de novo pathway was closely linked to PAICS on BTA6. These, and the other bovine locations i.e. GART at BTA1, PFAS at BTA19, ADSL at BTA5, ATIC at BTA2 and ADSS at BTA16, are in agreement with published comparative maps of cattle and man. PAICS and PPAT genes are known to be closely linked in human, rat and chicken. Previously, an expressed sequence fragment of PAICS (Bos taurus corpus luteum, BTCL9) was mapped to BTA13. By isolation and characterization of a BAC clone, we have now identified a PAICS processed pseudogene sequence (psiPAICS) on BTA13. Processed pseudogene sequences of PAICS and other genes of the purine biosynthesis pathway were identified in several mammalian species, indicating that the genes of this pathway have been susceptible to retrotransposition. The seven bovine genes are expressed at a higher level in testicular and ovary tissues compared with skeletal muscle.

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Section: Screening Of a Cdna And A Bac Library With Btcl9mentioning

confidence: 99%

“…2002), using the primers listed in Table 1. The mapping was performed and analysed as previously described (Bønsdorff et al. 2003).…”

Section: Rh Mappingmentioning

confidence: 99%

Section: Expression Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mapping of the bovine genes of the de novo AMP synthesis pathway¹

et al. 2004

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“…This approach has been used to accelerate gene discovery in a variety of bovine tissues including the ovary [14], mammary gland [15], embryo [16], foetus [17], and multiple tissues [18]. In addition, a recent study reported the identification and mapping of 11 gene sequences preferentially expressed in the bovine CL [19]. The generation of ESTs is vital to the growing area of bovine functional genomics and in facilitating large-scale gene expression studies [20,21], gene mapping, and the identification of genes influencing economically important traits [22,23].…”

Section: Introductionmentioning

confidence: 99%

Analysis of gene expression in the bovine corpus luteum through generation and characterisation of 960 ESTs

Casey

Fitzpatrick

McInerney

et al. 2004

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expres

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To gain new insights into gene identity and gene expression in the bovine corpus luteum (CL) a directionally cloned CL cDNA library was constructed, screened with a total CL cDNA probe and clones representing abundant and rare mRNA transcripts isolated. The 5V-terminal DNA sequence of 960 cDNA clones, composed of 192 abundant and 768 rare mRNA transcripts was determined and clustered into 351 non-redundant expressed sequence tag (EST) groups. Bioinformatic analysis revealed that 309 (88%) of the ESTs showed significant homology to existing sequences in the protein and nucleotide public databases. Several previously unidentified bovine genes encoding proteins associated with key aspects of CL function including extracellular matrix remodelling, lipid metabolism/steroid biosynthesis and apoptosis, were identified. Forty-two (12%) of the ESTs showed homology with human or with other uncharacterised ESTs, some of these were abundantly expressed and may therefore play an important role in primary CL function. Tissue-specificity and temporal CL gene expression of selected clones previously unidentified in bovine CL tissue was also examined. The most interesting finds indicated that mRNA encoding squalene epoxidase was constitutively expressed in CL tissue throughout the oestrous cycle and 7-fold down-regulated ( P < 0.05) in late luteal tissue, concomitant with the disappearance of systemic progesterone, suggesting that de novo cholesterol biosynthesis plays an important role in steroidogenesis. The mRNA encoding the growth factor, insulin-like growth factor-binding protein-related protein 1 (IGFBP-rP1), remained constant during the oestrous cycle and was 1.8-fold up-regulated ( P < 0.05) in late luteal tissue implying a role in CL regression. D

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