Identification and Physiological Characterization of a Consortium of Hydrocarbon-Oxidizing Bacteria of Oil and Oil Products

Abstract

A consortium of microorganisms were isolated from TC-1 fuel form, each member of which is capable of consistently degrade hydrocarbons’ different fractions. The 5 strains of hydrocarbon-oxidizing bacteria (PSB) were identified and isolated from TS-1 jet fuel. Their physiological and biochemical features are defined. All strains exhibit positive catalase activity. It is determined that all UOB strains, producing exogenous and endogenous surfactants, are capable to growth on media with different fraction of hydrocarbons. The study of these associations allows to create effective preparations for bioremediation in the elimination of accidental spills of oil and petroleum products.

Revealing of Non-Cultivable Bacteria Associated with the Mycelium of Fungi in the Kerosene-Degrading Community Isolated from the Contaminated Jet Fuel

“…In our previous works, thirteen bacterial strains were isolated from the contaminated TS-1 aviation kerosene by conventional microbiological methods on the Rich medium. Their taxonomic affiliation was verified by 16S rRNA sequencing [ 45 , 46 ]. Kerosene was characterized by a diverse bacterial composition that included the following genera: Sphingobacterium, Alcaligenes, Rhodococcus, and Deinococcus .…”

“…Kerosene was characterized by a diverse bacterial composition that included the following genera: Sphingobacterium, Alcaligenes, Rhodococcus, and Deinococcus . Isolated strains were capable of the degradation of hydrocarbons and production of biosurfactants [ 45 , 46 ]. At the same time, in this study, the same bacterial genera were indicated in monospore fungal cultures based on 16S rRNA metabarcoding.…”

“…In order to indicate the presence of cultured bacteria associated with the mycelium of the monospore fungal cultures, the following media were used: Rich medium [ 42 ] and Evans medium (EM) [ 43 ]. Previously, it was shown that EM and Rich medium are optimal for isolation of cultured heterotrophic bacteria from samples of contaminated oil and fuel [ 44 , 45 , 46 ]. All isolates of micromycetes were transferred either on liquid or solid media containing 2.0% (wt/ v ) agar.…”

Microbiological remediation of oil-contaminated soils

“…Important factors determining the effectiveness of the use of micromycetes as oil utilizers are their active enzymatic system and high viability in conditions of oil contamination [56]. Micromycetes-destructors of petroleum hydrocarbons include at least 100 species of microscopic fungi [57]. Patent documents for bioremediation techniques (most of them from China, the USA, Japan, and Taiwan [27]) mention more than 30 species of fungi.…”