Identification and Preliminary Characterization of p31, a New PSTAIRE-related Protein in Fission Yeast

Tournier, Sylvie; Gachet, Yannick; Hyams, Jeremy S.

doi:10.1002/(sici)1097-0061(19970630)13:8<727::aid-yea134>3.0.co;2-w

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…Antibody GJG56 was affinity purified as described previously (Olmsted, 1981) and used at 1:100 dilution. In S. pombe, the PSTAIRE antibody detects two PSTAIRE motif-containing proteins, p34 (Cdc2p) and p31, which encodes a PSTAIRE-related protein (Tournier et al, 1997). Primary antibodies were followed with the appropriate peroxidase-conjugated secondary antibody (Sigma).…”

Section: Immunoblottingmentioning

confidence: 99%

Thecdr2⁺Gene Encodes a Regulator of G₂/M Progression and Cytokinesis inSchizosaccharomyces pombe

Hudson

Balasubramanian

et al. 1998

MBoC

103

101

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Schizosaccharomyces pombe cells respond to nutrient deprivation by altering G2/M cell size control. The G2/M transition is controlled by activation of the cyclin-dependent kinase Cdc2p. Cdc2p activation is regulated both positively and negatively. cdr2(+) was identified in a screen for regulators of mitotic control during nutrient deprivation. We have cloned cdr2(+) and have found that it encodes a putative serine-threonine protein kinase that is related to Saccharomyces cerevisiae Gin4p and S. pombe Cdr1p/Nim1p. cdr2(+) is not essential for viability, but cells lacking cdr2(+) are elongated relative to wild-type cells, spending a longer period of time in G2. Because of this property, upon nitrogen deprivation cdr2(+) mutants do not arrest in G1, but rather undergo another round of S phase and arrest in G2 from which they are able to enter a state of quiescence. Genetic evidence suggests that cdr2(+) acts as a mitotic inducer, functioning through wee1(+), and is also important for the completion of cytokinesis at 36 degrees C. Defects in cytokinesis are also generated by the overproduction of Cdr2p, but these defects are independent of wee1(+), suggesting that cdr2(+) encodes a second activity involved in cytokinesis.

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Section: Immunoblottingmentioning

confidence: 99%

Thecdr2⁺Gene Encodes a Regulator of G₂/M Progression and Cytokinesis inSchizosaccharomyces pombe

Hudson

Balasubramanian

et al. 1998

MBoC

103

101

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“…Pef1 was originally identified as a kinase that contains a PSTAIRE motif, which is a highly conserved amino acid sequence of the CDK family (Tournier et al, 1997). There have been few studies that aimed to identify the function, activation mechanisms and downstream pathways of Pef1.…”

Section: Discussionmentioning

confidence: 99%

The S. pombe CDK5 ortholog Pef1 regulates sexual differentiation through control of the TORC1 pathway and autophagy

Matsuda

Kikkawa

Uda

et al. 2020

Journal of Cell Science

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In Schizosaccharomyces pombe, a general strategy for survival in response to environmental changes is sexual differentiation, which is triggered by TORC1 inactivation. However, mechanisms of TORC1 regulation in fission yeast remain poorly understood. In this study, we found that Pef1, which is an ortholog of mammalian CDK5, regulates the initiation of sexual differentiation through positive regulation of TORC1 activity. Conversely, deletion of pef1 leads to activation of autophagy and subsequent excessive TORC1 reactivation during the early phases of the nitrogen starvation response. This excessive TORC1 reactivation results in the silencing of the Ste11-Mei2 pathway and mating defects. Additionally, we found that pef1 genetically interacts with tsc1/2 in TORC1 regulation, and physically interacts with three types of cyclins, Clg1, Pas1, and Psl1. The double deletion of clg1 and pas1 promotes activation of autophagy and TORC1 during nitrogen starvation, similar to pef1Δ cells. Overall, our work suggests that Pef1-Clg1 and Pef1-Pas1 complexes regulate initiation of sexual differentiation through control of the TSC-TORC1 pathway and autophagy.

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“…5A,B). The antibody used is specific for the conserved PSTAIR motif and the extra band is presumably the Cdk p31 (Tournier et al, 1997).…”

Section: Cdc2 and Cdc37 Interact Geneticallymentioning

confidence: 99%

Activity of Cdc2 and its interaction with the cyclin Cdc13 depend on the molecular chaperone Cdc37 inSchizosaccharomyces pombe

Turnbull

Martin

Fantes

2006

Journal of Cell Science

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Cdc37 is a molecular chaperone whose clients are predominantly protein kinases, many of which are important in cell-cycle progression. Temperature-sensitive mutants of cdc37 in Schizosaccharomyces pombe are lethal at the restrictive temperature, arresting cell division within a single cell cycle. These mutant cells elongate during incubation at the restrictive temperature, consistent with a cell-cycle defect. The cell-cycle arrest arises from defective function of the mutant Cdc37 proteins rather than a reduction in Cdc37 protein levels. Around 80% of the arrested, elongated cells contain a single nucleus and replicated (2C) DNA content, indicating that these mutants arrest the cell cycle in G2 or mitosis (M). Cytological observations show that the majority of cells arrest in G2. In fission yeast, a G2 cell-cycle arrest can arise by inactivation of the cyclin-dependent kinase (Cdk) Cdc2 that regulates entry into mitosis. Studies of the cdc37 temperature-sensitive mutants show a genetic interaction with some cdc2 alleles and overexpression of cdc2 rescues the lethality of some cdc37 alleles at the restrictive temperature, suggesting that Cdc2 is a likely client for the Cdc37 molecular chaperone. In cdc37 temperature-sensitive mutants at the restrictive temperature, the level of Cdc2 protein remains constant but Cdc2 protein kinase activity is greatly reduced. Inactivation of Cdc2 appears to result from the inability to form complexes with its mitotic cyclin partner Cdc13. Further evidence for Cdc2 being a client of Cdc37 in S. pombe comes from the identification of genetic and biochemical interactions between these proteins.

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Identification and Preliminary Characterization of p31, a New PSTAIRE-related Protein in Fission Yeast

Cited by 8 publications

References 26 publications

Thecdr2⁺Gene Encodes a Regulator of G₂/M Progression and Cytokinesis inSchizosaccharomyces pombe

Thecdr2⁺Gene Encodes a Regulator of G₂/M Progression and Cytokinesis inSchizosaccharomyces pombe

The S. pombe CDK5 ortholog Pef1 regulates sexual differentiation through control of the TORC1 pathway and autophagy

Activity of Cdc2 and its interaction with the cyclin Cdc13 depend on the molecular chaperone Cdc37 inSchizosaccharomyces pombe

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Identification and Preliminary Characterization of p31, a New PSTAIRE-related Protein in Fission Yeast

Cited by 8 publications

References 26 publications

Thecdr2+Gene Encodes a Regulator of G2/M Progression and Cytokinesis inSchizosaccharomyces pombe

Thecdr2+Gene Encodes a Regulator of G2/M Progression and Cytokinesis inSchizosaccharomyces pombe

The S. pombe CDK5 ortholog Pef1 regulates sexual differentiation through control of the TORC1 pathway and autophagy

Activity of Cdc2 and its interaction with the cyclin Cdc13 depend on the molecular chaperone Cdc37 inSchizosaccharomyces pombe

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Product

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Thecdr2⁺Gene Encodes a Regulator of G₂/M Progression and Cytokinesis inSchizosaccharomyces pombe

Thecdr2⁺Gene Encodes a Regulator of G₂/M Progression and Cytokinesis inSchizosaccharomyces pombe