Identification and profiling of microbial community from industrial sludge

Abstract

This study aims to identify microbial communities and their taxonomical pro ling basis of order, species, genus, family, and class at the level in the sludge of pulp and paper industry. Studies showed dominant phyla in 16S rDNA Illumina Miseq analysis inside sludge were Anaerolinea, Pseudomonas, Clostridia, Bacteriodia, Gammaproteobacteria, Spirochetia, Deltaproteobacteria, Spirochaetaceae, Prolixibacteraceae and some unknown microbial strains are also dominant. The results of metabarcoding of the V3-V4 16S rRNA regions acquired from paired-end Illumina MiSeq sequencing were used to analyze bacterial communities and structure. Microorganisms can produce a vast variety of secondary metabolites, all of which are playing a crucial in biogeochemical cycle processes. The present work demonstrates the potential approach to sludge treatment in the open environment via the naturally adapted microorganism, which could be an essential addition to the disposal site. In summary, these investigations indicate that the indigenous microbial community is an acceptable bioresource for remediation or detoxi cation following secondary treatment. This research aims at understanding the structure of microbial communities and their taxonomic diversity (%) in highly contaminated sludge to perform in-situ bioremediation.

Gaoshiqia sediminis gen. nov., sp. nov., isolated from coastal sediment

“…At present, closely related strains in the genera Mangrovibacterium and Sunxiuqinia have been classified, which all originated from marine environments. Prolixibacteraceae has been detected in polycyclic aromatic hydrocarbon-contaminated coastal marine sediment, paper industry sludge and an aquaculture wastewater treatment system [13–15]. Prolixibacteraceae is beneficial to the degradation of complex organic matter, and can be used in bio-electrochemical technology to treat wastewater-containing complex organic matter, heavy metal and sulphate [16].…”