Identification and profiling of miRNAs in overwintering Lissorhoptrus oryzophilus via next-generation sequencing

Yang, Shuang; Zhang, Juhong; Wang, Shang; Zhang, Xinxin; Liu, Yan

doi:10.1016/j.cryobiol.2016.11.013

Cited by 14 publications

(7 citation statements)

References 55 publications

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“…In the present study, the down-regulated differentially expressed miRNAs (20) were much more than the up-regulated differentially expressed miRNAs (5) during diapause (D/PD), which was in accord with our iTRAQ proteome data that the differentially overexpressed proteins (82) were more than the differentially underexpressed proteins (57) during diapause [30], because miRNA abundance has most often been negatively correlated with expression of its target genes. Reynolds et al also got similar results that the majority of differentially expressed miRNAs (8) were down-regulated during diapause and only two miRNAs were up-regulated in diapausing pupae than in their non-diapausing counterparts in S. bullata [3].…”

Section: Discussionsupporting

confidence: 91%

“…MicroRNAs (miRNAs) are a class of small noncoding RNAs of approximately 22 nt in length, which regulate gene expression at the post-transcriptional level by targeting sites in mRNA, and have been shown to regulate a variety of physiological processes throughout insect development [4]. Up to date, thousands of miRNAs have been identi ed from insect species, including Drosophila melanogaster [5], Bombyx mori [6,7], Locusta migratoria [8], Manduca sexta [9][10][11], Plutella xylostella [12,13], Helicoverpa armigera [14,15], Mayetiola destructor [16], Nilaparvata lugens [17], Blattella germanica [18], Aphis gossypii [19], Lissorhoptrus oryzophilus [20], Leptinotarsa decemlineata [21], Grapholita molesta [22], Atrijuglans hetaohei [23], Apis cerana cerana [24], Laodelphax striatellus [25], and Plodia interpunctella [26]. More recent evidence has shown that miRNAs may play an important role in regulation of insect diapause.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Comparative Profiling of microRNAs at Different Diapause Stages of Galeruca Daurica Adults

Duan¹,

Li²,

Tan³

et al. 2020

Preprint

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Background: MicroRNAs (miRNAs) are a class of small noncoding RNAs of approximately 22 nt in length, which regulate gene expression at the post-transcriptional level. Although the regulatory roles of miRNAs in various physiological processes throughout insect development have been investigated, it is almost unknown about the roles of miRNAs involved in the regulation of diapause in insects.Results: We constructed 12 small RNA libraries from Galeruca daurica adults at different diapause stages: pre-diapause (PD), diapause (D), post-diapause 1 (TD1), and post-diapause 2 (TD2). Using Illumina sequencing, a total of 95.06 million valid reads was obtained, and 230 miRNAs, including 143 conserved and 87 novel miRNAs, were identified from G. daurica. The expression profiles of these miRNAs were assessed across different diapause stages and miRNAs that were highly expressed at different diapause stages were identified. Comparative analysis of read counts indicated that both conserved and novel miRNAs were differently expressed among the four different diapause stages, and the differential expression was validated via qRT-PCR. The 25, 11, 15, 14, 26, and one miRNAs were differentially expressed in D/PD, D/TD1, D/TD2, TD1/PD, TD2/PD, and TD2/TD1, respectively. The KEGG and GO analysis of the predicted target genes suggested the essential roles of miRNAs in the regulation of summer diapause in G. daurica, especially via the juvenile hormone, ribosome, MAPK signaling, mTOR signaling, Ca2+ signaling, and G-protein coupled receptor signaling pathways.Conclusion: Our research results indicate that miRNAs may be involved in the regulation of summer diapause in G. daurica, and these results also provide an important new small RNA genomics resource for further studies on insect diapause.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Identification and Comparative Profiling of microRNAs at Different Diapause Stages of Galeruca Daurica Adults

Duan¹,

Li²,

Tan³

et al. 2020

Preprint

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“…We downloaded 2,474 insect miRNA sequences from miRbase 22.0 and 517 miRNAs from publicly reported references (Chang et al ., ; Yang et al ., ), covering 14 species across five insect orders. To amass more insect miRNA information, we sequenced small RNA libraries of three notorious insect pests, Scirpophaga incertulas (yellow stem borer, YSB), Nilaparvata lugens (brown planthopper, BPH) and Laodelphax striatellus (small brown planthopper, SBPH), yielding 76, 110 and 118 miRNAs, respectively (Tables S1 and S2).…”

Section: Resultsmentioning

confidence: 99%

Transgenic microRNA‐14 rice shows high resistance to rice stem borer

Xiao

Sun

et al. 2018

Plant Biotechnology Journal

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Rice stem borer (RSB, Chilo suppressalis) is an insect pest that causes huge economic losses every year. Control efforts rely heavily on chemical insecticides, which leads to serious problems such as insecticide resistance, environment pollution, and food safety issues. Therefore, developing alternative pest control methods is an important task. Here, we identified an insect-specific microRNA, miR-14, in RSB, which was predicted to target Spook (Spo) and Ecdysone receptor (EcR) in the ecdysone signalling network. In-vitro dual luciferase assays using HEK293T cells confirmed the interactions of Csu-miR-14 with CsSpo and with CsEcR. Csu-miR-14 exhibited high levels of expression at the end of each larval instar stage, and its expression was negatively correlated with the expression of its two target genes. Overexpression of Csu-miR-14 at the third day of the fifth instar stage led to high mortality and developmental defects in RSB individuals. We produced 35 rice transformants to express miR-14 and found that three lines had a single copy with highly abundant miR-14 mature transcripts. Feeding bioassays using both T and T generations of transgenic miR-14 rice indicated that at least one line (C#24) showed high resistance to RSB. These results indicated that the approach of miRNAs as targets has potential for improving pest control methods. Moreover, using insect-specific miRNAs rather than protein-encoding genes for pest control may prove benign to non-insect species, and thus is worthy of further exploration.

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“…Multiple studies have reported the differential expression of small noncoding RNAs in insects exposed to varying temperatures. Examples include the identification of a signature of miRNAs that displayed changing levels in the overwintering L. oryzophilus ( Yang et al 2017 ) as well as profiling the differentially expressed miRNAs in larvae of the freeze-tolerant fly Eurosta solidaginis (Diptera: Tephritidae) ( Lyons et al 2016 ). Transcript levels of Ago1 and Ago2, key molecular players underlying miRNA and siRNA function, were also shown to be differentially expressed in response to select temperature treatments in the brown planthopper Laodelphax striatellus (Hemiptera: Delphacidae) ( Zhou et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…This supports their likely role in regulating adaptation to a variety of conditions including cold temperatures. Accordingly, modulation of a signature of 36 miRNAs in the overwintering rice water weevil, Lissorhoptrus oryzophilus (Coleoptera: Erirhinidae) ( Yang et al 2017 ), as well as the potential role of miR-31-5p during cold acclimation in the wood-boring beetle Monochamus alternatus (Coleoptera: Cerambycidae) ( Zhang et al 2020 ), are select examples of work that highlighted the potential importance of miRNAs in insect cold response. In addition, it is relevant to add that several studies have shown the importance of miRNAs in insects exposed to various insecticides.…”

mentioning

confidence: 99%

Exposure to Temperature and Insecticides Modulates the Expression of Small Noncoding RNA-Associated Transcripts in the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Youssef

Ouédraogo

Bastarache

et al. 2022

Journal of Insect Science

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The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is an insect that can adapt to various challenges, including temperature fluctuations or select insecticide treatments. This pest is also an ongoing threat to the potato industry. Small noncoding RNAs such as miRNAs, which can control posttranscriptionally the expression of various genes, and piRNAs, which can notably impact mRNA turnover, are modulated in insects under different conditions. Unfortunately, information regarding the expression status of key players involved in their synthesis and function is for the most part lacking. The current study thus aims at assessing the levels of such targets in L. decemlineata exposed to hot and cold temperatures as well as treated to the insecticides chlorantraniliprole, clothianidin, imidacloprid, and spinosad. Transcript expression levels of Ago1, Ago2, Ago3, Dcr2a, Dcr2b, Expo-5, Siwi-1, and Siwi-2, components of pathways associated with small noncoding RNA production or function, were measured by qRT-PCR and revealed modulation of select transcripts in response to temperature challenges and to select insecticides. RNAi-mediated reduction of Ago2 transcript levels in L. decemlineata injected with Ago2-targeting dsRNA and exposed to cold and warm temperatures was also conducted. Changes in survival rates were observed for the latter condition in dsRNA- versus saline-injected insects. These results showcase the differential expression of select targets involved in small noncoding RNA homeostasis and provide leads for the subsequent assessment of their involvement during stress response in L. decemlineata using RNAi-based approaches.

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Identification and profiling of miRNAs in overwintering Lissorhoptrus oryzophilus via next-generation sequencing

Cited by 14 publications

References 55 publications

Identification and Comparative Profiling of microRNAs at Different Diapause Stages of Galeruca Daurica Adults

Identification and Comparative Profiling of microRNAs at Different Diapause Stages of Galeruca Daurica Adults

Transgenic microRNA‐14 rice shows high resistance to rice stem borer

Exposure to Temperature and Insecticides Modulates the Expression of Small Noncoding RNA-Associated Transcripts in the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

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Identification and profiling of miRNAs in overwintering Lissorhoptrus oryzophilus via next-generation sequencing

Cited by 14 publications

References 55 publications

Identification and Comparative Profiling of microRNAs&nbsp;at Different Diapause Stages of Galeruca Daurica Adults

Identification and Comparative Profiling of microRNAs&nbsp;at Different Diapause Stages of Galeruca Daurica Adults

Transgenic microRNA‐14 rice shows high resistance to rice stem borer

Exposure to Temperature and Insecticides Modulates the Expression of Small Noncoding RNA-Associated Transcripts in the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

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Identification and Comparative Profiling of microRNAs at Different Diapause Stages of Galeruca Daurica Adults

Identification and Comparative Profiling of microRNAs at Different Diapause Stages of Galeruca Daurica Adults