Identification and purification of a novel adhesion-associated protein in a new strain of Lactobacillus, L15, from flounder (Paralichthys olivaceus)

Chen, Ying; Wang, Lei; Li, Jia-Zhong; Zhan-tao, Shao; An, Liguo

doi:10.1016/j.vetmic.2006.12.009

Cited by 12 publications

(6 citation statements)

References 26 publications

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“…This is due in large part because there is specificity in colonization by the strain-host complex or vice versa. Ying et al (2007) observed that Lactobacillus adhesion to the surface of the intestinal tract of P. olivaceus depends on the specificity of the host strain. Given the above, we suggest that the microbial community of wild P. adspersus provides a suitable environment for the adhesion and colonization of this type of bacteria, and that the LAB isolated in this study could be studied as a specific probiotic to improve production traits in AF.…”

Section: Discussionmentioning

confidence: 98%

Characterization of the intestinal microbiota of wild caught and farmed fine flounder (Paralichthys adspersus)

Salas‐Leiva

Opazo

Remond

et al. 2017

lajar

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ABSTRACT.A comparative analysis of the cultivable intestinal microbiota of farmed (AF) and wild-caught (WF) Paralichthys adspersus was performed. The 16S rRNA gene was used for taxa identification, and the ITS region for strain differentiation. We detected the presence of Vibrio, Bacillus, Photobacterium, Staphylococcus and Carnobacterium in AF, and Exiguobacterium, Klebsiella, Arthrobacter, Raoultella, Kluyvera, Myroides, Streptococcus, Vagococcus, Staphylococcus, Acinetobacter, Psychrobacter, Lactobacillus, Weissella and Lactococcus in WF. The microbial community was more diverse in WF than in AF. Some bacterial groups were only found in wild-caught fish and may be studied as potential beneficial agents for improving production traits in farmed fish. As the first study of microbiota of P. adspersus, it provides significant information that can potentially help improve farming practices by using strains as species-specific probiotics.

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Section: Discussionmentioning

confidence: 98%

Characterization of the intestinal microbiota of wild caught and farmed fine flounder (Paralichthys adspersus)

Salas‐Leiva

Opazo

Remond

et al. 2017

lajar

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“…Several studies reported that indigenous bacteria are more beneficial than bacteria isolated from other sources (Nayak, 2010 ; Mills et al, 2011 ). This may be due at least in part to the specificity in colonization by the host-strain relationship (Ying et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

The Microbiome of Seriola lalandi of Wild and Aquaculture Origin Reveals Differences in Composition and Potential Function

Ramı́rez

Romero

2017

Front. Microbiol.

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Seriola lalandi is an economically important species that is globally distributed in temperate and subtropical marine waters. Aquaculture production of this species has had problems associated with intensive fish farming, such as disease outbreaks or nutritional deficiencies causing high mortalities. Intestinal microbiota has been involved in many processes that benefit the host, such as disease control, stimulation of the immune response, and the promotion of nutrient metabolism, among others. However, little is known about the potential functionality of the microbiota and the differences in the composition between wild and aquacultured fish. Here, we assayed the V4-region of the 16S rRNA gene using high-throughput sequencing. Our results showed that there are significant differences between S. lalandi of wild and aquaculture origin (ANOSIM and PERMANOVA, P < 0.05). At the genus level, a total of 13 genera were differentially represented between the two groups, all of which have been described as beneficial microorganisms that have an antagonistic effect against pathogenic bacteria, improve immunological parameters and growth performance, and contribute to nutrition. Additionally, the changes in the presumptive functions of the intestinal microbiota of yellowtail were examined by predicting the metagenomes using PICRUSt. The most abundant functional categories were those corresponding to the metabolism of cofactors and vitamins, amino acid metabolism and carbohydrate metabolism, revealing differences in the contribution of the microbiota depending on the origin of the animals. To our knowledge, this is the first study to characterize and compare the intestinal microbiota of S. lalandi of wild and aquaculture origin using high-throughput sequencing.

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“…This may be due to the fact that collagen is a single component and it has fewer interactions with bacterial surface proteins, whereas there are multiple proteins in mucus that can interact with bacterial surface proteins, but both interactions cannot fully explain the actual adhesion mechanism of lactic acid bacteria in the organism. The adhesion characteristics were common to many S-layer proteins (Strus et al, 2001 ; Ying et al, 2007 ). Furthermore, we identified that the surface proteins of Elongation factor Tu, Type I glyceraldehyde-3-phosphate dehydrogenase, Small heat shock protein, and 30S ribosomal are possible to interact with the shrimp intestinal proteins by Western blotting.…”

Section: Discussionmentioning

confidence: 99%

Adhesion and Colonization of the Probiotic Lactobacillus plantarum HC-2 in the Intestine of Litopenaeus Vannamei Are Associated With Bacterial Surface Proteins

Shao

et al. 2022

Front. Microbiol.

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Surface proteins are a type of proteins expressed on the surface of bacteria that play an important role in cell wall synthesis, maintenance of cell morphology, and signaling with the host. Our previous study showed that the probiotic Lactobacillus plantarum HC-2 improved the growth performance and immune response of Litopenaeus vannamei. To further investigate the probiotic mechanism, we determined the automatic aggregation ability of the bacteria and surface hydrophobicity of HC-2 after being treated with 5 M of lithium chloride (LiCl) and observed the morphology and adhesion of the bacteria to HCT116 cells. The results showed that with the removal of the HC-2 surface protein, the auto-aggregation ability and surface hydrophobicity of HC-2 decreased, and the crude mucus layer coated on the bacterial surface gradually dissociated. The adhesion rate of HC-2 to HCT116 cells decreased from 98.1 to 20.9%. Moreover, a total of 201 unique proteins were identified from the mixture of the surface proteins by mass spectrometry (MS). Several proteins are involved in transcription and translation, biosynthetic or metabolic process, cell cycle or division, cell wall synthesis, and emergency response. Meanwhile, a quantitative real-time PCR qPCR_ showed that HC-2 was mainly colonized in the midgut of shrimp, and the colonization numbers were 15 times higher than that in the foregut, while the colonization rate in the hindgut was lower. The adhesion activity measurement showed that the adhesion level of HC-2 to crude intestinal mucus of L. vannamei was higher than that of bovine serum albumin (BSA) and collagen, and the adhesion capacity of the bacterial cells decreased with the extension of LiCl-treatment time. Finally, we identified the elongation factor Tu, Type I glyceraldehyde-3-phosphate dehydrogenase, small heat shock protein, and 30S ribosomal protein from the surface proteins, which may be the adhesion proteins of HC-2 colonization in the shrimp intestine. The above results indicate that surface proteins play an important role in maintaining the cell structure stability and cell adhesion. Surface proteomics analysis contributes to describing potential protein-mediated probiotic-host interactions. The identification of some interacting proteins in this work may be beneficial to further understand the adhesion/colonization mechanism and probiotic properties of L. plantarum HC-2 in the shrimp intestine.

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Identification and purification of a novel adhesion-associated protein in a new strain of Lactobacillus, L15, from flounder (Paralichthys olivaceus)

Cited by 12 publications

References 26 publications

Characterization of the intestinal microbiota of wild caught and farmed fine flounder (Paralichthys adspersus)

Characterization of the intestinal microbiota of wild caught and farmed fine flounder (Paralichthys adspersus)

The Microbiome of Seriola lalandi of Wild and Aquaculture Origin Reveals Differences in Composition and Potential Function

Adhesion and Colonization of the Probiotic Lactobacillus plantarum HC-2 in the Intestine of Litopenaeus Vannamei Are Associated With Bacterial Surface Proteins

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