Identification and Quantification of Aconitines and Colchicine in Serum, Urine, and Plants using MonoSpin C18 and LC-MSMS

Saito, Takeshi; Umebachi, Rimako; Namera, Akira; Miyazaki, Shota; Fujita, Yuji; Inokuchi, Sadaki

doi:10.1007/s10337-015-2921-z

Cited by 11 publications

(3 citation statements)

References 28 publications

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“…To compare our results with other studies [ 5 , 6 , 26 ], the validation data in the previous studies were listed in Table S4, where common analytes among the previous studies and the present study were shown. To compare the results appropriately, the validation data for almost the same QC concentration were extracted.…”

Section: Resultsmentioning

confidence: 88%

“…These quantitative values are almost the same as the mean maximum concentration (6.0 ± 3.4 ng/mL) of colchicine in plasma obtained from 20 male volunteers, ingested a single 2.0-mg colchicine tablet on the pharmacokinetic study [ 37 ]. Furthermore, Saito et al reported that the serum concentration of colchicine in an actual poisoning case was 6.1 ng/mL [ 26 ]. Additionally, Abe et al reported that the colchicine concentration in patient plasma in a fatal case of colchicine self-poisoning was 60 ng/mL [ 38 ], remarkably higher than the LLOQ level of our method.…”

Section: Resultsmentioning

confidence: 99%

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Easily Operable Quantification Method of 21 Plant-Derived Alkaloids in Human Serum by Automatic Sample Preparation and Liquid Chromatography–Tandem Mass Spectrometry

Taniguchi¹,

Takamura

Watanabe

et al. 2022

Chromatographia

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In this study, we developed an easily operable quantification method for 21 plant-derived alkaloids in human serum by automatic sample preparation and liquid chromatography–tandem mass spectrometry. We designed to perform parallel sample preparation by a developed apparatus, which increased sample throughput. We conducted an automatic sample preparation through de-proteinization with 0.1% formic acid in methanol and achieved recovery rates of 89–107% (2.0–14% RSD) for all targeted analytes, demonstrating its high repeatability. The method validation results were satisfactory as follows: the linearity ( r 2 ) of each calibration curve ranged from 0.978 to 1.000; the inter- and intra-day accuracies were 89.0–125% and 82.1–110%, respectively; the inter- and intra-day precisions were below 13% and 10%, respectively. Additionally, the lower limits of detection and quantification were 0.0044–0.047 and 0.013–0.14 ng/mL, respectively. Finally, the developed method was applied to pseudo-protoveratrine A poisoning serum and pseudo-colchicine poisoning serum, which were prepared by diluting acute-poisoning mice serum with human serum. Our method successfully quantitated protoveratrine A (0.15–0.25 ng/mL) and colchicine (4.8–6.0 ng/mL). Thus, our method is essential for prompt clinical treatment and critical care on patient in acute intoxication cases caused by plant-derived alkaloids. Supplementary Information The online version contains supplementary material available at 10.1007/s10337-022-04212-5.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Easily Operable Quantification Method of 21 Plant-Derived Alkaloids in Human Serum by Automatic Sample Preparation and Liquid Chromatography–Tandem Mass Spectrometry

Taniguchi¹,

Takamura

Watanabe

et al. 2022

Chromatographia

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“…Considering these mentioned facts, the rapid and sensitive detection of colchicine from a variety of samples is of profound significance in the pharmaceutical, clinical, food, and breeding industries. To date, there have been many analytical methods developed for the detection of colchicine, like thin layer chromatography–densitometer, HPLC using UV or MS detection, liquid chromatography–tandem mass spectrometry, and electrochemical analysis, ,− but these methods are generally time-consuming, expensive, and cumbersome. Therefore, it is necessary and urgent to find a highly efficient and sensitive method to detect colchicine.…”

mentioning

confidence: 99%

Luminescent Detection of Colchicine by a Unique Indium–Organic Framework in Water with High Sensitivity

et al. 2019

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Colchicine is highly toxic to creatures, and sensitively detecting colchicines is of profound significance in the pharmaceutical, clinical, food, and breeding industries. We herein designed and constructed a unique luminescent indium–organic framework {[(CH3)2NH2][In(L)]·9DMF} n (V105) via an in situ ligand-mediated method, which possesses a 2-fold interpenetrated 3D anionic framework. Due to the large channels that exist in the framework with the size of 12 Å × 14 Å, V105 can rapidly remove harmful cationic dyes from water in a few minutes. Importantly, luminescent experiments demonstrate that V105 can selectively detect colchicine with high sensitivity in water, and the limit of detection can reach 1.0 × 10–7 M. To our knowledge, this is the first example of a metal–organic framework-based luminescent sensor for detecting colchicine.

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Monolithic Spin Column Extraction in LC‐MS Bioanalysis

Namera¹,

Saito²

2019

Sample Preparation in LC‐MS Bioanalysis

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Identification and Quantification of Aconitines and Colchicine in Serum, Urine, and Plants using MonoSpin C18 and LC-MSMS

Cited by 11 publications

References 28 publications

Easily Operable Quantification Method of 21 Plant-Derived Alkaloids in Human Serum by Automatic Sample Preparation and Liquid Chromatography–Tandem Mass Spectrometry

Easily Operable Quantification Method of 21 Plant-Derived Alkaloids in Human Serum by Automatic Sample Preparation and Liquid Chromatography–Tandem Mass Spectrometry

Luminescent Detection of Colchicine by a Unique Indium–Organic Framework in Water with High Sensitivity

Monolithic Spin Column Extraction in LC‐MS Bioanalysis

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