Identification and Quantification of Amino Acids From Medicinally Important Plants by Using High-Performance Thin-Layer Chromatography

Abstract

& The present paper deals with simultaneous identification and quantitative determination of five amino acids, viz., L-lysine, L-threonine, L-valine, L-isoleucine, and L-tryptophan using highperformance thin-layer chromatographic (HPTLC) technique in five different medicinal plants, viz., Amoora rohitaka (Roxb.), Adiantum capillus-veneris, Sphaeranthus hirtus Willd., Swertia chirata (Wall.), and Malaxis acuminata. The separation was performed on TLC aluminium plates precoated with silica gel 60 F 254 . Good separation was achieved in the mobile phase of 1-butanol=glacial acetic acid=water (4:2:2) and densitometric determination of these compounds was carried out at 254 nm in absorbance mode. The results provide valuable information about the presence of essential amino acids. Threonine is found in all the analyzed samples in the range of 145 ng=spot to 343 ng=spot. Adiantum capillus-veneris contains the highest amount of amino acid (1.57 mg=g) among the plants under study.

Distribution, Chemical Constituents and Biological Properties of Genus Malaxis

“…[5,22,26] Fresh bulb paste of M. muscifera and M. acuminata is applied to cure phthisis, bronchitis, bleeding diathesis, internal and external hemorrhage, burning sensation, spermopiotic, and fever by the folks of Nagaland, India. [28][29] In folk medicine, the pseudobulbs are utilized in bronchitis and as a tonic. Similarly, pseudobulbs of M. muscifera are used for curing fever, as well as haemostatic, antidiarrhoeal, styptic, antidysenteric, and vitiated conditions of the pitta and vata, and are also recommended in the treatment of joint arthritis.…”

“…Various sugar molecules and their glycosides like galactose (14), glucose (15), D-glucose (16), D (+) sucrose (17), D-mannose (18), fructose (19), β-maltose (20), α-D glucopyranose (21), levoglucosan (22), methyl β-D-glucofuranoide (23), β-galactopyranose (24), gluconolactone (25), α-D-(+) -mannopyranose (26) and rhamnose (27) were obtained from M. acuminata. Also, the structural investigation of M. acuminata revealed various monosaccharides like galactose (14), glucose (15), mannose (18), arabinose (28), βmaltose (20) etc. [41] These compounds possess significant antiinflammatory, antioxidant, and immune-modulating properties.…”

“…Various ayurvedic formulations, for example, Astavarga Churna, Chyawanprash Rasayana, Mahapadma Oil, Chitakaditaila, Vajikaranghirta, Mahamauryaghirta, HaritakKyadiyoga, Bramha Rasayana and Himvanaagada contains Malaxis as an essential ingredient [5,22,26] . Fresh bulb paste of M. muscifera and M. acuminata is applied to cure phthisis, bronchitis, bleeding diathesis, internal and external hemorrhage, burning sensation, spermopiotic, and fever by the folks of Nagaland, India [28–29] . In folk medicine, the pseudobulbs are utilized in bronchitis and as a tonic.…”