Identification and quantification of bacterial genomes carrying antibiotic resistance genes and virulence factor genes for aquatic microbiological risk assessment

Liang, Jinsong; Mao, Guannan; Yin, Xiaole; Ma, Liping; Liu, Lei; Bai, Yaohui; Zhang, Tong; Qu, Jiuhui

doi:10.1016/j.watres.2019.115160

Cited by 134 publications

(56 citation statements)

References 39 publications

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“…These results also showed significant difference of pathogenic composition among the three bays. XB had higher proportion of potential pathogens, frequently containing genes for virulence factors ( Liang et al, 2020 ). Of course, the identification of pathogens from 16S rRNA gene sequence mainly depends on limited reference database in this study.…”

Section: Resultsmentioning

confidence: 99%

“…Based on the analysis of bacterial genomes, Proteobacteria and Firmicutes species were also highly enriched with many ARGs ( Hu et al, 2016 ). In addition, the stronger connectivity between potential pathogenic genera and piARGs in the gXB indicated that pathogens tended to acquire ARGs likely due to their exposure to higher concentrations of antibiotics ( Liang et al, 2020 ). The networks also indicated that some piARG subtypes ( tetA , aacA , aacC , aadK , cusR , etc.)…”

Section: Resultsmentioning

confidence: 99%

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Evaluating the Potential Antibiotic Resistance Status in Environment Based on the Trait of Microbial Community

Huang

et al. 2020

Front. Microbiol.

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The overuse of antibiotics has promoted the propagation and dissemination of antibiotic resistance genes (ARGs) in environment. Due to the dense human population and intensive activities in coastal areas, the health risk of ARGs in coastal environment is becoming a severe problem. To date, there still lacks of a quantitative method to assess properly the gross antibiotic resistance at microbial community level. Here, we collected sediment samples from Hangzhou Bay (HB), Taizhou Bay (TB), and Xiangshan Bay (XB) of the East China Sea for community-level ARGs analysis. Based on the 16S rRNA genes and predictive metagenomics, we predicted the composition of intrinsic ARGs (piARGs) and some related functional groups. Firstly, a total of 40 piARG subtypes, belonging to nine drug classes and five resistance mechanisms, were obtained, among which the piARGs encoding multidrug efflux pumps were the most dominant in the three bays. Secondly, XB had higher relative abundances of piARGs and pathogens than the other two bays, which posed higher potential health risk and implied the heavier impact of long-term maricultural activities in this bay. Thirdly, the co-occurrence network analysis identified that there were more connections between piARGs and some potential pathogenic bacteria. Several piARG subtypes (e.g., tetA , aacA , aacC , and aadK ) distributed widely in the microbial communities. And finally, the microbial diversity correlated negatively with the relative abundance of piARGs. Oil, salinity, and arsenic had significant effects on the variations of piARGs and potential pathogenic bacteria. The abundance-weighted average ribosomal RNA operon ( rrn ) copy number of microbial communities could be regarded as an indicator to evaluate the antibiotic resistance status. In conclusion, this study provides a new insight on how to evaluate antibiotic resistance status and their potential risk in environment based on a quantitative analysis of microbial communities.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Evaluating the Potential Antibiotic Resistance Status in Environment Based on the Trait of Microbial Community

Huang

et al. 2020

Front. Microbiol.

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“…This probabilistic approach firstly consists of the evaluation of viral infections via oral path, i.e., the host and virus interaction via cellular receptors with and without conquering the host defense system and replication in the host cell (Voysey and Brown, 2000;Huang and Haas, 2009), followed by designing the models to study disease progress immediately after infection. The virulence markers are the viral gene sequences representing the viral disease-related traits (Haddad et al, 2018;Liang J. et al, 2020). Therefore, it is observed that the above-discussed appeal must be undertaken for coronavirus probabilistic virulence assessment (Benvenuto et al, 2020).…”

Section: Role Of Omics Techniques For Virulence Assessmentmentioning

confidence: 99%

“…niaid.nih.gov), an integrated surveillance data for supporting researchers in collaboration with various research institutes working on systems biology (Squires et al, 2012;Uyeki et al, 2016). The main aim is to make the comparative genomic research for correlative analysis of coronaviridae family-related genus and strains for the predicted data and annotated genome sequences (Zhu et al, 2016;Liang J. et al, 2020;Randhawa et al, 2020). In this process, the MSA (Multiple Sequence Alignment) signifies the closely related RefSeq constructing the virus ortholog groups with associated protein playing role in virulence (Fumagalli et al, 2010;Chapman et al, 2011;Claytor et al, 2017).…”

Section: Genomicsmentioning

confidence: 99%

Systems Biology Approaches for Therapeutics Development Against COVID-19

Jaiswal

Kumar

Mandeep

et al. 2020

Front. Cell. Infect. Microbiol.

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Understanding the systems biology approaches for promoting the development of new therapeutic drugs is attaining importance nowadays. The threat of COVID-19 outbreak needs to be vanished for global welfare, and every section of research is focusing on it. There is an opportunity for finding new, quick, and accurate tools for developing treatment options, including the vaccine against COVID-19. The review at this moment covers various aspects of pathogenesis and host factors for exploring the virus target and developing suitable therapeutic solutions through systems biology tools. Furthermore, this review also covers the extensive details of multiomics tools i.e., transcriptomics, proteomics, genomics, lipidomics, immunomics, and in silico computational modeling aiming towards the study of host-virus interactions in search of therapeutic targets against the COVID-19.

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“…E o surgimento da atual pandemia está correlacionada com as síndromes de ARDS, SARS e MERS e as mutações relacionadas ao atual coronavírus (covid19) decorre de animais infectados tendo os morcegos como agente transmissor, onde o SARS-CoV-2 é um vírus de RNA com característica de fita única com um genoma de ∼ 30 quilobases que codificam múltiplas proteínas estruturais e é pertencente ao Coronaviridae família do betacoronavírus do gênero tipo 2 (Liang et. al.…”

Section: Introductionunclassified

Recursos fisioterapêuticos utilizados no Pós-COVID 19: Uma revisão bibliográfica

Nagamine

Lourenço

Chaves

2021

RSD

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Pacientes pós-COVID-19 em sua grande maioria necessita de reabilitação fisioterapêutica durante e logo após a internação segundo a Organização Mundial da Saúde. E o programa de reabilitação fisioterapêutico possui uma ênfase e maior importância no sistema cardiorrespiratório. O objetivo desse artigo é demonstrar como os recursos fisioterapêuticos podem auxiliar na melhora desses pacientes Pós-Covid 19 independente do estado em que se encontra, seja ele leve, moderado ou grave, a partir das pesquisas realizadas nas plataformas digitais: Scielo, Pubmed, Associações Internacionais com os descritores coronavírus, fisiopatologia e tratamento, durante o período de 6 meses. Diante das pesquisas realizadas pode-se concluir que a reabilitação fisioterapêutica através de recursos como: thresold (aparelho com carga linear pressórica), RPPI (respiração com pressão positiva intermitente), ressuscitador manual e exercícios resistidos conseguem melhorar a capacidade cardiorrespiratória e promover uma maior tolerância ao recondicionamento dos pacientes Pós-Covid 19, consequentemente melhorando a qualidade de vida.

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Identification and quantification of bacterial genomes carrying antibiotic resistance genes and virulence factor genes for aquatic microbiological risk assessment

Cited by 134 publications

References 39 publications

Evaluating the Potential Antibiotic Resistance Status in Environment Based on the Trait of Microbial Community

Evaluating the Potential Antibiotic Resistance Status in Environment Based on the Trait of Microbial Community

Systems Biology Approaches for Therapeutics Development Against COVID-19

Recursos fisioterapêuticos utilizados no Pós-COVID 19: Uma revisão bibliográfica

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