Identification and Quantification of Carotenoids, By HPLC-PDA-MS/MS, from Amazonian Fruits

Abstract

The major and minor carotenoids from six fruits, buriti (Mauritia vinifera), mamey (Mammea americana), marimari (Geoffrola striata), peach palm (Bactrys gasipaes), physalis (Physalis angulata), and tucuma (Astrocaryum aculeatum), all native to the Amazonia region, were determined by high-performance liquid chromatography-photodiode array detector-mass spectrometry detector (HPLC-PDA-MS/MS), fulfilling the recommended criteria for identification. A total of 60 different carotenoids were separated on a C30 column, all-trans-beta-carotene being the major carotenoid found in all fruits. The presence of apo-10'-beta-carotenol, found in mamey, was not previously reported in foods. In addition, this is the first time that the identification of beta-zeacarotene in natural sources is supported by MS data. The total carotenoid content ranged from 38 microg/g in marimari to 514 microg/g in buriti. All fruits analyzed can be considered good sources of provitamin A, especially buriti, with 7280 RE/100 g.

Efeito da Astrocaryum aculeatum (Tucumã) na toxicidade da Doxorrubicina: modelo experimental in vivo

“…The pulp is well appreciated, and consumed in natura by the population, or as a filling in sandwiches, tapiocas, custard, ice cream, and flour. (4) de Rosso & Mercadante (5) described 24 carotenoids; 21 were chemically identified in the tucumã. Tucumã analysis show one of the highest concentrations of pro-vitamin A, representing 52 mg/100 g of pulp, with a potential antioxidant that block the harmful effects caused by free radicals.…”

“…Tucumã analysis show one of the highest concentrations of pro-vitamin A, representing 52 mg/100 g of pulp, with a potential antioxidant that block the harmful effects caused by free radicals. Carotenoids are essential for cell differentiation, embryonic development, vision, as well as many other functions, including potential therapeutic benefits (5,6) According to Ambrósio et al, (2006), (7) β-carotene is a powerful antioxidant that protects against cardiovascular diseases by inhibiting the oxidative process of low-density lipoprotein (LDL). Important polyphenols, such as quercetin, are also present in tucumã, which is one of the main flavonoids present in the human diet.…”

Desenvolvimento e validação de método espectrofotométrico para determinação de corante à base de luteína adicionado em iogurte desnatado

“…O extrato remanescente foi seco, redissolvido em éter de petróleo e saponificado com igual volume de solução metanólica 10% de KOH. 16 Após retirada do álcali, o extrato saponificado foi seco e analisado nas mesmas condições utilizadas para o extrato bruto (não saponificado).…”

“…Os espectros de absorção UV-visível foram adquiridos entre 200 e 600 nm e os cromatogramas foram processados a 450 nm. 17 Os picos foram identificados com base na ordem de eluição em coluna C 18 e nas seguintes características do espectro UV-visível: l max , estrutura fina espectral calculada como percentagem das alturas (III/II)% e intensidade do pico cis calculada como (A B /A II )%, assinaladas na Figura 1, 16 comparando com informações disponíveis na literatura. 15,[17][18][19] …”

“…16,22,23 Para os testes com acetona, o uso de mesa agitadora foi descartado. Foram realizadas extrações em homogeneizador e almofariz, ambas resultando em valores de recuperação de carotenoides totais inferiores a 80%.…”