Identification and Quantification of Carotenoids Including Geometrical Isomers in Fruit and Vegetable Juices by Liquid Chromatography with Ultraviolet−Diode Array Detection

C, Cortés Vizcaíno; Esteve, María J.; Frígola, Ana; Torregrosa, Francisco

doi:10.1021/jf035505y

Cited by 66 publications

(56 citation statements)

References 48 publications

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“…Although certain xanthophylls are sensitive to saponification, moderate saponification could be employed. Cortes et al (2004) have shown that 30 min incubation at room temperature with 5 mmol of KOH per sample was sufficient to carry out complete saponification of retinol-palmitate. Consequently, in the present study, our gentle saponification step, in line with those used by others (Association of Official Analytical Chemists 1990; Mercadante and Rodriguez-Amaya 1998;Lee et al 2001), produces a chromatographic profile in agreement with the literature where lutein and the violaxanthin are the xanthophylls mainly found in spinach, followed by b-carotene (Larsen and Christisen 2005).…”

Section: Discussionmentioning

confidence: 99%

Development of a method to determine carotenoid composition of fresh forages

Cardinault¹,

Lyan²,

Doreau³

et al. 2008

Can. J. Plant Sci.

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Development of a method to determine carotenoid composition of fresh forages. Can. J. Plant Sci. 88: 1057Á1064. Due to the limited interest in carotenoids in ruminant diets until recently, analyses of forages are often incomplete, focusing mainly on b-carotene and lutein. Carotenoid composition of green forage from middle mountain meadow was analyzed by HPLC after extraction and elimination of chlorophylls by mild saponification. This method of analysis uses two C 18 columns in series with a quaternary gradient system. Our method allowed, for the first time, the identification and quantification of several xanthophylls other than lutein (i.e., violaxanthin, antheraxanthin, epilutein) in chlorophyll-free extracts from carotenoidrich forage. The intra-day (3.5Á7.5%) and inter-day (1.2Á3.5%) coefficients of variation are suitable for routine determination of carotenoids in green forage. This method could also be used in metabolic studies of these micronutrients in ruminants.Key words: Xanthophylls, carotenoids, fresh forage, HPLC Cardinault, N., Lyan, B., Doreau, M., Chauveau, B., Rock, E. et Grolier, P. 2008. De´veloppement d'une me´thode afin de de´terminer la composition en carote´noı¨des de fourrages frais. Can. J. Plant Sci. 88: 1057Á1064. En raison de l'inte´reˆt limitej usqu'a`maintenant pour les carote´noı¨des dans les re´gimes des ruminants, les analyses sont souvent incomple`tes et se focalisant principalement sur le b-carote`ne et la lute´ine. La composition en carote´noı¨des d'un fourrage vert d'une prairie de moyenne montagne a e´te´analyse´e par CLHP apre`s extraction et e´limination des chlorophylles par une saponification mode´re´e. Cette me´thode d'analyse utilise deux colonnes C18 en se´rie avec un syste`me de gradient quaternaire. Notre me´thode a permis pour la premie`re fois d'identifier et de quantifier plusieurs autres xanthophylles (violaxanthine, antheraxanthine, epiluteine) que la luteine dans un extrait de fourrage riche en carote´noı¨des de´pourvu de chlorophylles Les coefficients de variations intra-jour (3,5%-7,5%) et inter-jour (1,2%-3,5%) sont adapte´s pour une analyse courante des carote´noı¨des dans les fourrages verts. Cette me´thode pourrait aussi eˆtre utilise´e dans des e´tudes me´taboliques sur ces micronutriments chez les ruminants.

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Section: Discussionmentioning

confidence: 99%

Development of a method to determine carotenoid composition of fresh forages

Cardinault¹,

Lyan²,

Doreau³

et al. 2008

Can. J. Plant Sci.

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show abstract

“…Carotenoids were extracted and quantified by HPLC, following a procedure validated by Cortés, Esteve, Frígola, and Torregrosa (2004), with some modifications.…”

Section: Carotenoid Compoundsmentioning

confidence: 99%

Changes on phenolic and carotenoid composition of high intensity pulsed electric field and thermally treated fruit juice–soymilk beverages during refrigerated storage

et al. 2011

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“…Extracts of citrus fruit peel and pulp are an important and complex source of carotenoids, which are natural pigments, in considerable amounts and in all species. bcryptoxanthin and b-carotene were identified on the basis of comparing their retention times and absorption spectrum characteristics with corresponding authentic standards (Cortés et al, 2004;Bermejo et al, 2011b).…”

Section: Fruit Quality: Nutritional Qualitymentioning

confidence: 99%

Murcott seedless: influence of gamma irradiation on citrus production and fruit quality

Bermejo

Pardo

Caño

2012

Span. j. agric. res.

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A Seedlessness is an important economic trait relating to fruit quality, and gamma irradiation is a common technique used to obtain seedless citrus fruits. Herein, we report a study of new seedless 'Murcott' mandarin clones obtained by bud irradiation from the self-compatible not parthenocarpic 'Murcott' mandarin. All irradiated clones examined presented lower seed numbers (from 0.23 to 2.47 seeds per fruit) and reduced pollen germination (from 1.40% to 8.55%) whereas the wild-type 'Murcott' showed an average number of 9.03 seeds per fruit and a pollen germination value of 47.15%. Fruit quality and nutritional bio-components were affected differently; some clones presented no changes compared to the control 'Murcott' mandarin, while other clones showed significant differences. High-performance liquid chromatographic methods were used to identify and quantify of these compounds, using photodiode array, mass and refractive index detectors. Our results indicated high contents in natural antioxidants as vitamin C (from 20.13 to 25.73 mg/100 mL) and phenolic compounds, as flavonoids, in these citrus varieties cultived under the Mediterranean climate. Some of these clones, which ripen late in the season and whose fruit quality is maintained or improved, are in the process of registration. In conclusion, budwood irradiation is a suitable technique to improve cultivars, produce seedless cultivars, adjust ripening time or raise the content of health-promoting compounds. Also this study investigates the influence of temperature during flowering on the number of seeds formed. Findings indicate that low temperatures during flower formation decreased pollen germination and seed number.Additional key words: citrus clones; mandarin; nutritional bio-components; pollen germination; temperature. ResumenMurcott sin semillas: influencia de la irradiación gamma sobre la producción de cítricos y la calidad de la fruta El carácter ''sin semillas'' es un rasgo importante relacionado con la calidad de la fruta, y la inducción de mutaciones mediante irradiaciones gamma es una técnica común utilizada para obtener frutos sin semillas de cítricos. Presentamos aquí el estudio realizado en los nuevos clones que hemos obtenido mediante irradiación, a partir de la variedad autocompatible y no partenocárpica 'Murcott'. Todos los clones irradiados examinados presentaron menor número de semillas (de 0,23 a 2,47 semillas por fruto) y reducción de la germinación del polen (de 1,40% a 8,55%), mientras que la mandarina 'Murcott' control mostró 9,03 semillas por fruto y 47,15% de germinación del polen. La calidad de la fruta y el contenido en compuestos bioactivos y nutricionales se vieron afectados de manera diferente, algunos clones no presentaron cambios con respecto al control, mientras que otros clones sí mostraron diferencias significativas. Nuestros resultados indican un alto contenido en antioxidantes naturales como la vitamina C (20,13 a 25,73 mg/100 mL) y compuestos fenólicos, como flavonoides, en estas variedades de cítricos cultivad...

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Identification and Quantification of Carotenoids Including Geometrical Isomers in Fruit and Vegetable Juices by Liquid Chromatography with Ultraviolet−Diode Array Detection

Cited by 66 publications

References 48 publications

Development of a method to determine carotenoid composition of fresh forages

Development of a method to determine carotenoid composition of fresh forages

Changes on phenolic and carotenoid composition of high intensity pulsed electric field and thermally treated fruit juice–soymilk beverages during refrigerated storage

Murcott seedless: influence of gamma irradiation on citrus production and fruit quality

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