2003
DOI: 10.1038/nbt827
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Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry

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Cited by 1,356 publications
(1,488 citation statements)
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References 39 publications
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“…One such enrichment strategy is the enrichment of N‐glycosylated peptides and the subsequent PNGase F‐catalyzed conversion of Asn to Asp using solid state extraction method 90, 91 a method which was successfully applied to serum samples 75, 92. Upon purification, only the modified peptide is quantified.…”
Section: Clinical Applicationsmentioning
confidence: 99%
“…One such enrichment strategy is the enrichment of N‐glycosylated peptides and the subsequent PNGase F‐catalyzed conversion of Asn to Asp using solid state extraction method 90, 91 a method which was successfully applied to serum samples 75, 92. Upon purification, only the modified peptide is quantified.…”
Section: Clinical Applicationsmentioning
confidence: 99%
“…Consortium member Rudi Aebersold from the ETH developed one such method using mass spectrometry that separates out glycoproteins -the proteins involved in key processes such as protein folding or cell-cell signalling -from the background of other proteins 1 . Importantly, the technique quantifies as well as identifies proteins, providing a molecular fingerprint for a cell in a particular stage of health or disease.…”
Section: The Meaning Of Lifementioning
confidence: 99%
“…Unlike N-linked glycosylation where proteomic and enrichment methods have been available, O-linked glycosylation remains challenging to study. 28,29 …”
Section: Introductionmentioning
confidence: 99%