Identification and quantification of n-octyl esters of alkanoic and hexanedioic acids and phthalates as urban wastewater markers in biota and sediments from estuarine areas

Chaler, Roser; Cantón, L.; Vaquero, M. Carmen; Grimalt, Joan O.

doi:10.1016/j.chroma.2004.06.098

Cited by 8 publications

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several rivers and estuary areas acquire land-based contaminants, such as industrial and municipal sewage. The monitoring of these ecosystems is important to identify the occurrence of possibly hazardous pollutants and their adverse influences on the coastal environment, hence the need to provide the necessary data for possible environmental management and preservation [4].…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Assessment of Organic Pollutants Residues in the Aquatic Environment of the Coastal Sediments

2023

View full text Add to dashboard Cite

The current study aimed to monitor organic pollution on island and coastal environments using linear alkylbenzene (LAB). The aquatic environment is affected by the hazardous chemicals discharged through domestic and industrial waste. The distribution, composition, and sources of LABs in the sediments of Port Dickson coast and Pulau Merambong were identified using gas chromatography–mass spectrometry (GC–MS). Chains ranging from long to short (L/S), C13/C12 homologs, and internal to external (I/E) congeners were used to define the degradation rate of LABs and the efficacy of wastewater treatment plants. The results of this study revealed that the concentration of LABs in the sites under investigation varied from 67.4 in Pulau Merambong to 255.8 ng g−1dw, in Port Dickson. The LAB homologs had a significant difference and a significant percentage of sampling stations had C13-LAB homologs. According to the determined LAB ratios (I/E), which ranged from 1.6 in Pulau Merambong to 4.1 in Port Dickson, treated effluents from primary and secondary inputs are being introduced into the aquatic ecosystem of these areas. The degradation of LABs was up to 64% in the interrogated locations. The conclusion is that the wastewater treatment system needs to be improved, and that LAB molecular markers are highly effective in tracing anthropogenic sewage contamination.

show abstract

Section: Introductionmentioning

confidence: 99%

Occurrence and Assessment of Organic Pollutants Residues in the Aquatic Environment of the Coastal Sediments

2023

View full text Add to dashboard Cite

show abstract

“…Moreover, wastewaters often contain relevant concentrations of PAEs (Tran et al, 2022), which are partly transported to the sea by river runoff. Among PAEs, di(2-ethylhexyl)phthalate (DEHP) is the most widely produced and used, as well as the most persistent phthalate found in seawaters (Chaler et al, 2004;Bergéet al, 2013). Indeed, DEHP was reported to reach a high concentration in the marine environment, up to 11,500 μg/kg dw in marine sediments, 4.35 μg/L in marine water, and 1,573 μg/kg ww in marine fishes (Stewart et al, 2014;Hermabessiere et al, 2017;Zhang et al, 2018;Malem et al, 2019;Hu et al, 2020).…”

mentioning

confidence: 99%

Plastic additive di(2-ethylhexyl)phthalate (DEHP) causes cell death and micronucleus induction on a bottlenose dolphin’s (Tursiops truncatus) in vitro-exposed skin cell line

Giovani

Filippi²,

Molino³

et al. 2022

Front. Mar. Sci.

View full text Add to dashboard Cite

Marine plastic pollution is one of the most concerning worldwide environmental issues, and research is day by day demonstrating its adverse effects on marine ecosystems. Nevertheless, little is still known about the toxic potential on marine fauna of chemical additives released by plastic debris. Here we investigated the cyto- and genotoxicity of the most used plasticizer in plastic production, di(2-ethylhexyl)phthalate (DEHP), on a skin cell line (TT) derived from the bottlenose dolphin (Tursiops truncatus), a species particularly exposed to the accumulation of this lipophilic pollutant, being a coastal top predator rich in fatty subcutaneous tissues. Dolphin cell cultures were exposed to increasing DEHP doses (0.01–5 mM) to evaluate effects on cell viability, cell death, and induction of DNA damage. On the hypothesis that bottlenose dolphin cells show greater resistance to DEHP toxicity than terrestrial mammals, as already shown for other pollutants, the same parameters were analyzed on exposed Chinese hamster ovary (CHO) cell lines. Both MTT and Trypan Blue assays showed no significant decrease in dolphin’s cell viability after 24-h DEHP exposure. No induction of primary DNA damage was detected by the comet assay, whereas the cytokinesis-block micronucleus assay revealed significant micronuclei induction and inhibition of cell proliferation starting from the lowest DEHP doses. DEHP had similar but sharper and significant effects on cell viability in CHO cells, also causing a much greater induction of necrosis than that recorded on dolphin cells. For both cell lines, the lack of induction of primary DNA damage (i.e., strand breaks) together with the increase of micronuclei yield after DEHP treatment suggests an aneugenic effect of the phthalate, that is, the loss of entire chromosomes during cell division. Overall, the potential chromosome loss detected could constitute a threat for species of marine mammals constantly exposed to plastic marine litter.

show abstract

Accumulation of di(2‐ethylhexyl) phthalate causes endocrine‐disruptive effects in marine medaka (Oryzias melastigma) embryos

Kang

Huang

et al. 2014

Environmental Toxicology

View full text Add to dashboard Cite

Di (2-ethylhexyl) phthalate (DEHP) is extensively distributed in marine environments. However, limited research on the toxicological and molecular effects of DEHP on marine organisms has been conducted. Our study investigated the accumulation, elimination, and endocrine-disruptive effects of DEHP on embryonic marine medaka (Oryzias melastigma). The medaka embryos were continuously exposed to DEHP (0.01, 0.1, and 1 mg/L) or 17β-estradiol (E2, 0.01 mg/L) until hatching, and the newly hatched larvae were then transferred to clean sea water for 12 days of depuration. DEHP and E2 appeared to have no significant effects on the mortality and hatching rates of medaka embryos, but E2 exposure significantly delayed the hatching. Significantly higher DEHP embryonic burdens were detected in the group treated with higher DEHP (0.1 and 1 mg/L) at 10 dpf (days post fertilization). The recovered larvae showed an elimination tendency of DEHP during the recovery period. DEHP had no significant effects on the transcriptional responses of endocrine-disrupting biomarker genes in the 3-dpf embryos. Treatment with 0.1 and 1 mg/L DEHP elicited a significant induction of transcriptional responses of ER, PPAR, and the CYP19 genes in a concentration-dependent manner at 10 dpf, indicating endocrine disruption may be due to bioaccumulation of DEHP. With the elimination of DEHP during the depuration period, all of the effects on these genes showed no significant effects. However, 0.1 mg/L E2 significantly affected the expression of ER, PPAR, and the CYP19 genes in the exposed embryos at both 3 and 10 dpf and recovered larvae. Therefore, these results demonstrate that accumulation of DEHP caused endocrine disruption in medaka embryos and that recovery in clean sea water may weaken the endocrine-disrupting effects.

show abstract

Identification and quantification of n-octyl esters of alkanoic and hexanedioic acids and phthalates as urban wastewater markers in biota and sediments from estuarine areas

Cited by 8 publications

References 36 publications

Occurrence and Assessment of Organic Pollutants Residues in the Aquatic Environment of the Coastal Sediments

Occurrence and Assessment of Organic Pollutants Residues in the Aquatic Environment of the Coastal Sediments

Plastic additive di(2-ethylhexyl)phthalate (DEHP) causes cell death and micronucleus induction on a bottlenose dolphin’s (Tursiops truncatus) in vitro-exposed skin cell line

Accumulation of di(2‐ethylhexyl) phthalate causes endocrine‐disruptive effects in marine medaka (Oryzias melastigma) embryos

Contact Info

Product

Resources

About