Identification and quantification of Nɛ-(Hexanoyl)lysine in human urine by liquid chromatography/tandem mass spectrometry

Kato, Yoji; Yoshida, Akihiro; Naito, Michitaka; Kawai, Yoshichika; Tsuji, Kentaro; Kitamura, Masayasu; Kitamoto, Noritoshi; Osawa, Toshihiko

doi:10.1016/j.freeradbiomed.2004.09.007

Cited by 38 publications

(26 citation statements)

References 27 publications

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“…The levels of the measured concentrations of the markers correspond to ranges identified in previously published data, which were measured on the different groups of healthy, non-pregnant subjects (11,12,16,25,26). The concentrations of the markers vary between studies.…”

Section: Discussionsupporting

confidence: 68%

“…The concentrations of the markers vary between studies. These studies in healthy non-pregnant women reported that the urinary HEL levels were between 1.58±0.23 and 2.3±1.2 µmol/mol Cr (11,12,16), PRL levels were 21.6±10.6 µmol/mol Cr (11), DiY levels were between 8.8±0.6 and 10.1±0.4 µmol/mol Cr (16,25) and NY levels were between 0.46±0.49 and 1.4±0.4 µmol/mol Cr (16,26).…”

Section: Discussionmentioning

confidence: 97%

“…HEL is formed via the reaction of peroxidised n-6 fatty acid with protein lysine residues, while PRL is generated through the reaction of n-3 fatty acid with these residues (9). Previous studies have suggested that HEL levels are elevated in the urine of patients with diabetes (10)(11)(12), metabolic syndrome (13), rheumatoid arthritis (14) and systemic sclerosis (15). Oxidative damage to proteins may alter various levels of the protein structure and activity.…”

Section: Introductionmentioning

confidence: 99%

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Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Rejc

Kato

Karas-Kuzelicki

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Pregnancy is a physiological state accompanied by excessive levels of oxidative stress (OS), due to the increased demand and utilisation of oxygen. There is increasing evidence that maternally augmented OS exerts an adverse effect on pregnancy outcome. The aim of the present prospective study was to determine the association between the urinary concentration of relatively novel OS markers measured in the second trimester of pregnancy and the infant characteristics at birth. The maternal levels of urinary hexanoyl-lysine (HEL), propanoyl-lysine (PRL), dityrosine (DiY) and 3-nitrotyrosine (NY) were evaluated in generally healthy pregnant subjects to determine their association with birth weight, gestation at delivery and Apgar score. The observed levels of the markers were in agreement with those measured in healthy non-pregnant subjects in a previous study. A positive correlation was detected between HEL and PRL, as well as between HEL and DiY. Although the absence of a correlation between NY and the other markers has been previously noted in a non-pregnant population, a positive correlation in the pair PRL-NY (r=0.367; P<0.001) was observed in the present study. Maternal cigarette smoking was associated with increased urinary PRL levels (P=0.034). The most notable observation in the present study was that high levels of PRL and NY were associated with low Apgar scores at 1 and 5 min after birth (OR, 1.098 and 2.084 for PRL and NY, respectively; P<0.05). However, poor predictive accuracy was shown. For NY, the following results were obtained: Area under the curve (AUC), 0.818; sensitivity, 100%; specificity, 57%; positive predictive value (PPV), 11.54%; and negative predictive value (NPV), 100%.For PLR the values were as follows: AUC, 0.802; sensitivity, 100%; specificity, 62.6%; PPV, 13.05%; and NPV, 100%. DiY was negatively associated with preterm birth risk (OR=0.703; P=0.028). In conclusion, the results of the present study indicated the presence of OS in the second trimester of pregnancy, which was detected with damage to lipids and proteins and associated with an adverse Apgar score; however, the selected urinary markers exhibited poor positive predictive efficacy.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Rejc

Kato

Karas-Kuzelicki

et al. 2016

Experimental and Therapeutic Medicine

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“…HEL is a useful biomarker for initial stage of lipid peroxidation, which can be detected in human atherosclerotic lesions, urine, serum, and tears. 44 It should be noted though that the increase in tear HEL levels might not necessarily be a toxic change resulting from lipid peroxidation, but may be a physiological response to an increased reactive oxygen species burden. …”

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confidence: 99%

Alterations of the tear film and ocular surface health in chronic smokers

Matsumoto

Doğru

Goto

et al. 2008

Eye

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Objective To investigate the effects of chronic smoking on ocular surface and tear functions. Methods Fifteen right eyes of 15 healthy chronic smokers (9 men, 6 women; age range: 36-47 years) who smoked 20 cigarettes per day for 20 years and 20 eyes of 20 control nonsmokers (12 men, 8 women; age range: 38-43 years) were included in this prospective study. All subjects underwent measurements of breath and haemoglobin CO concentration, tear lipid layer interferometry, evaporimetry, tear film break-up time (TBUT), Schirmer's I test, corneal fluorescein staining, conjunctival impression, and brush cytology. Results The mean Hb CO level was significantly higher in smokers compared to non-smokers. TBUT was also significantly shorter in smokers. Tear lipid layer showed significant slowing in spread over the tear film with a concomitant significant increase in tear evaporation rate. Conjunctival impression cytology revealed significant loss of goblet cells and squamous metaplasia in smokers. Brush cytology showed significant conjunctival neutrophil infiltration in smoker subjects. Conclusion Chronic smoking induced distinctive quantitative and qualitative disturbances on the ocular surface health.

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“…HEL is considered to be one of the stable oxidative stress markers for lipid peroxide and protein modi cation [2]. An important role for oxidative stress in the increased HEL seen in diabetes is suggested by experimental and clinical studies [3,4].…”

mentioning

confidence: 99%

Oxidative lipid, protein, and DNA damage as oxidative stress markers in vascular complications of diabetes mellitus

Tabak¹,

Gelışgen²,

Erman³

et al. 2011

CIM

113

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Oxidative lipid, protein, and DNA damage as oxidative stress markers in vascular complications of diabetes mellitus AbstractPurpose: e purpose of this study was to determine the e ects of diabetic complications on oxidation of proteins, lipids, and DNA and to investigate the relationship between oxidative damage markers and clinical parameters.Methods: e study group consisted of 69 type 2 diabetic patients (20 patients without complication, 49 patients with complication) who attended internal medicine outpatient clinics of Istanbul Education and Research Hospital and 19 healthy control subjects. In serum samples of both diabetic patients and healthy subjects, 8-hydroxy-2'deoxyguanosine (8-OHdG), as a marker of oxidative DNA damage, N ε -(hexanoyl)lysine (HEL) and 15-F2t-iso-prostaglandin (15-F2t-IsoP). as products of lipooxidative damage, advanced oxidation protein products (AOPP), as markers of protein damage, and paraoxonase1 (PON1) as antioxidant were studied.Results: 15-F2t-IsoP (p<0.005) and AOPP (p<0.001) levels were signi cantly higher in diabetic group than control group while there were no signi cant di erences in levels of 8-OHdG and HEL between the two groups. AOPP (p<0.001) and 8-OHdG (p<0.001) were signi cantly higher in diabetic group with complications compared to diabetic group without complications.Conclusions: Increased formation of free radicals and oxidative stress, under conditions of hyperglycaemia, is one of the probable causes for evolution of complications in diabetes mellitus. Our study supports the hypothesis that oxidant/antioxidant balance is disturbed in diabetic patients.

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Identification and quantification of Nɛ-(Hexanoyl)lysine in human urine by liquid chromatography/tandem mass spectrometry

Cited by 38 publications

References 27 publications

Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Alterations of the tear film and ocular surface health in chronic smokers

Oxidative lipid, protein, and DNA damage as oxidative stress markers in vascular complications of diabetes mellitus

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