Identification and quantification of novel cranberry-derived plasma and urinary (poly)phenols

Feliciano, Rodrigo P.; Boeres, Albert; Massacessi, Luca; Istas, Geoffrey; Ventura, M. Rita; Santos, Cláudia N.; Heiß, Christian; Rodriguez-Mateos, Ana

doi:10.1016/j.abb.2016.01.014

Cited by 137 publications

(153 citation statements)

References 69 publications

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“…Other than previous work by our group where the same 60 metabolites were quantified in a single concentration of cranberry juice [22], this is a much larger number of metabolites than usually reported after cranberry juice consumption [23,24,25]. Further, here we present quantities of glucuronidated and sulfated metabolites in addition to the methylated and native metabolites, unlike many of the previous works looking at cranberry (poly)phenol metabolites.…”

Section: Discussionmentioning

confidence: 87%

“…A total of 60 compounds in plasma were identified and quantified post-consumption of cranberry juice containing 409, 787, 1238, 1534 and 1910 mg total (poly)phenols, as we have previously reported for the juice containing 787 mg of (poly)phenols [22]. These included cinnamic acids, dihydrocinnamic, flavonols, benzoic acids, phenylacetic acids, benzaldehydes, valerolactones, hippuric acids, catechols, and pyrogallols.…”

Section: Resultsmentioning

confidence: 99%

“…Further, fecal metabolites were not considered and it is possible that a big proportion of metabolites are lost via this route, in particular the high-molecular-weight proanthocyanidins, which are not absorbed to a big extent. The method of quantification of proanthocyanidins in cranberry that we used can also explain in part the low recovery, as previously discussed [22]. Here we used the OS4-dimethylaminocinnamaldehyde (DMAC) method rather than the often used BL DMAC method.…”

Section: Discussionmentioning

confidence: 99%

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Absorption, Metabolism and Excretion of Cranberry (Poly)phenols in Humans: A Dose Response Study and Assessment of Inter-Individual Variability

et al. 2017

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The beneficial health effects of cranberries have been attributed to their (poly)phenol content. Recent studies have investigated the absorption, metabolism and excretion of cranberry (poly)phenols; however, little is known about whether they follow a dose response in vivo at different levels of intake. An acute double-blind randomized controlled trial in 10 healthy men with cranberry juices containing 409, 787, 1238, 1534 and 1910 mg total (poly)phenols was performed. Blood and urine were analyzed by UPLC-Q-TOF-MS. Sixty metabolites were identified in plasma and urine including cinnamic acids, dihydrocinnamic, flavonols, benzoic acids, phenylacetic acids, benzaldehydes, valerolactones, hippuric acids, catechols, and pyrogallols. Total plasma, but not excreted urinary (poly)phenol metabolites, exhibited a linear dose response (r2 = 0.74, p < 0.05), driven by caffeic acid 4-O-ß-d-glucuronide, quercetin-3-O-ß-d-glucuronide, ferulic acid 4-O-ß-d-glucuronide, 2,5-dihydroxybenzoic acid, 2,4-dihydroxybenzoic acid, ferulic acid, caffeic acid 3-O-ß-d-glucuronide, sinapic acid, ferulic acid 4-O-sulfate, 3-hydroxybenzoic acid, syringic acid, vanillic acid-4-O-sulfate, (4R)-5-(3′-hydroxyphenyl)-γ-valerolactone-4′-O-sulfate, 4-methylgallic acid-3-O-sulfate, and isoferulic acid 3-O-sulfate (all r2 ≥ 0.89, p < 0.05). Inter-individual variability of the plasma metabolite concentration was broad and dependent on the metabolite. Herein, we show that specific plasma (poly)phenol metabolites are linearly related to the amount of (poly)phenols consumed in cranberry juice. The large inter-individual variation in metabolite profile may be due to variations in the gut microbiome.

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Section: Discussionmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Absorption, Metabolism and Excretion of Cranberry (Poly)phenols in Humans: A Dose Response Study and Assessment of Inter-Individual Variability

et al. 2017

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“…The chemical structures of these compounds have been previously presented [23]. Water, methanol, acetic acid and acetonitrile (HPLC grade) were purchased from Fisher Scientific (Loughborough, UK).…”

Section: Methodsmentioning

confidence: 99%

“…Highly unstable anthocyanins are degraded into smaller compounds as early as 1 hour postconsumption, before or after being absorbed in the small intestine, leading to a vast array of phenolic metabolites that can be glucuronidated, sulfated and/or methylated and circulate in much higher concentrations than the structurally related anthocyanin metabolites [19,22,23]. Many of the (poly)phenols present in berries are not absorbed in the small intestine and they reach the colon intact, where they are degraded by the gut microbiota into smaller phenolic metabolites that are absorbed, metabolized and appear in circulation at later timepoints [9,19,23].…”

Section: Introductionmentioning

confidence: 99%

Bioavailability of wild blueberry (poly)phenols at different levels of intake

Rodriguez-Mateos

Feliciano

Cifuentes-Gomez

et al. 2016

JBR

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Abstract. BACKGROUND:Data from human intervention studies have highlighted potential cardiovascular benefits of blueberry (poly)phenols. However, such biological effects are dependent on their bioavailability and, as such, information on the absorption, metabolism and excretion of such compounds is necessary. OBJECTIVE: To investigate whether the bioavailability of blueberry (poly)phenols is intake-dependent, a group of nine healthy volunteers consumed three wild blueberry drinks containing 766 mg (lower), 1278 mg (medium) and 1791 mg (higher) total (poly)phenols, corresponding to 34, 56 and 80 g, respectively, freeze-dried blueberry powder. METHODS: Plasma levels of (poly)phenol metabolites were assessed at baseline and at 1, 2, 4 and 6 hours post-consumption, using UPLC-Q-TOF mass spectrometry. RESULTS: Twenty-three phenolic acid metabolites were quantified in plasma after blueberry consumption. Increases in plasma (poly)phenol metabolites were observed in all the interventions tested. The area under the curve of the concentration over time (AUC) significantly increased when comparing the lower and higher (poly)phenol interventions. Linear doseresponse regressions were obtained for 11 metabolites, while the plasma concentration of the remaining 12 metabolites was not affected by increasing amounts of (poly)phenols in the blueberry interventions. CONCLUSION: Absorption and metabolism of blueberry (poly)phenols are not exclusively intake-dependent at the amounts tested, evidencing a complex metabolic fate of these compounds.

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