Identification and quantification of phytochelatins in roots of rice to long-term exposure: evidence of individual role on arsenic accumulation and translocation

Batista, Bruno Lemos; Nigar, Meher; Mestrot, Adrien; Rocha, Bruno Alves; Barbosa, Fernando; Price, Adam H.; Raab, Andrea; Feldmann, Jörg

doi:10.1093/jxb/eru018

Cited by 155 publications

(83 citation statements)

References 52 publications

(85 reference statements)

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“…Samples were treated according to Batista et al (2014). The determination of Al, Si, P, K, Ca, Mg, B, Mn, Fe, Cu, Zn and Ni was carried out using an inductively coupled plasma mass spectrometer (ICP-MS, Agilent 7900, Santa Clara, USA).…”

Section: Etermining Photosynthetic Pig Mentsmentioning

confidence: 99%

Silicon mitigates oxidative stress and has positive effects in Eucalyptus platyphylla under aluminium toxicity

Lima¹,

Júnior²,

Barbosa³

et al. 2016

PLANT SOIL ENVIRON

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Positive interactions to silicon (Si) were reported in Solanum nigrum plants (Liu et al. 2013) exposed to toxic cadmium (Cd) levels, while Kidd et al. (2001) also described that Si mitigates the toxicity by aluminium (Al) in Zea mays. In addition, the protective action of Si was observed in Oryza sativa under high levels of zinc (Zn) (Song et al. 2014), Hordeum vulgare under high concentrations of chromium (Cr) and Cucumis sativus exposed to manganese (Mn) toxicity (Shi et al. 2005).The tolerance to metal toxicity can be induced by the compartmentalization, chelation and/or immobilization of metal ions into the vacuole, cytoplasm, apoplast or/and cell wall. In addition, Britez et al. (2002) described that Si decreased Al toxicity due to the formation of Al-Si complexes in the apoplast, which are non-toxic forms. Based on this overview, our hypothesis is that Si must reduce the negative impacts on metabolism provoked by the action of Al in Eucalyptus platyphylla plants. The aim of this research is to determine the Si contribution on nutrient concentrations, compounds linked to oxidative stress, photosynthetic pigments and gas exchange, and determine if Si can improve the tolerance mechanism of young E. platyphylla plants exposed to Al toxicity. MATERIAL AND METHODS Plants ABSTRACTThe tolerance to metal toxicity, such as aluminium, can be induced by the formation of Al-Si complexes. Therefore, the aim of this research is to determine the contribution of Si on nutrient concentrations, compounds linked to oxidative stress, photosynthetic pigments and gas exchange and to determine if Si can improve the tolerance mechanism of young Eucalyptus platyphylla plants exposed to Al toxicity. The experimental setup was completely randomized with four treatments (0, 1.6 mmol/L Al, 2.0 mmol/L Si and 1.6 mmol/L Al + 2.0 mmol/L Si; being described as the control, Al toxicity, Si and Al toxicity + Si, respectively). The treatment with Si attenuated the negative effects of Al on nutrient concentrations by reducing superoxide, hydrogen peroxide, malondialdehyde and electrolyte leakage. Pigments and gas exchange exhibited beneficial effects after Si application, with positive interactions also being detected between Si-Al. Therefore, this study demonstrates that Si reduced the oxidative stress and improved the tolerance mechanism of young E. platyphylla plants exposed to Al toxicity.

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Section: Etermining Photosynthetic Pig Mentsmentioning

confidence: 99%

Silicon mitigates oxidative stress and has positive effects in Eucalyptus platyphylla under aluminium toxicity

Lima¹,

Júnior²,

Barbosa³

et al. 2016

PLANT SOIL ENVIRON

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“…As shown in Table 2, wheat root As was found to be positively correlated with root Cd (Table 2). One pos- sible reason for this is the different levels of phytochelatin (PC) synthesis in different wheat cultivars under As and Cd exposure [49,50]. Cultivars with a higher PC production may have higher As and Cd tolerance, as well as a higher capacity for As and Cd accumulation [50][51][52].…”

Section: Grain Asmentioning

confidence: 99%

“…One pos- sible reason for this is the different levels of phytochelatin (PC) synthesis in different wheat cultivars under As and Cd exposure [49,50]. Cultivars with a higher PC production may have higher As and Cd tolerance, as well as a higher capacity for As and Cd accumulation [50][51][52]. In addition, some metal transporters such as NRAMP1 (Natural Resistance-Associated Macrophage Protein) maybe contribute to the enhancement of both As and Cd accumulation in plants.…”

Section: Grain Asmentioning

confidence: 99%

The transportation and accumulation of arsenic, cadmium, and phosphorus in 12 wheat cultivars and their relationships with each other

Shi

Zhu

Bai

et al. 2015

Journal of Hazardous Materials

103

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“…7 (Grill et al, 2002;Klapheck 1988). Leguminosae (Klapheck et al, 1995), graminae (Batista et al, 2014;Oven et al, 2002), maize (Chassaigne et al, 2001), horseradish (Kubota et al, 2000) and some species of fungi (Collin-Hansen et al, 2007) have been found to produce hGSH and hPC in addition to GSH/PC (Mounicou et al, 2001), which can give an even greater redox buffering capacity than that observed for C. vulgaris.…”

Section: Arsenic (Iii) Effects In C Vulgarismentioning

confidence: 99%

The mechanisms of detoxification of As(III), dimethylarsinic acid (DMA) and As(V) in the microalga Chlorella vulgaris

et al. 2016

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The response of Chlorella vulgaris when challenged by As(III), As(V) and dimethylarsinic acid (DMA) was assessed through experiments on adsorption, efflux and speciation of arsenic (reduction, oxidation, methylation and chelation with glutathione/phytochelatin [GSH/PC]). Our study indicates that at high concentrations of phosphate (1.62 mM of HPO4 2− ), upon exposure to As(V), cells are able to shift towards methylation of As(V) rather than PC formation. Treatment with As(V) caused a moderate decrease in intracellular pH and a strong increase in the concentration of free thiols (GSH). Passive surface adsorption was found to be negligible for living cells exposed to DMA and As(V). However, adsorption of As(III) was observed to be an active process in C. vulgaris, because it did not show saturation at any of the exposure periods. Chelation of As(III) with GS/PC and to a lesser extent hGS/hPC is a major detoxification mechanism employed by C. vulgaris cells when exposed to As(III). The increase of bound As-GS/PC complexes was found to be strongly related to an increase in concentration of As(III) in media. C. vulgaris cells did not produce any As-GS/PC complex when exposed to As(V). This may indicate that a reduction step is needed for As(V) complexation with GSH/PC. C. vulgaris cells formed DMASV -GS upon exposure to DMA independent of the exposure period. As(III) triggers the formation of arsenic complexes with PC and homophytochelatins (hPC) and their compartmentalisation to vacuoles. A conceptual model was devised to explain the mechanisms involving ABCC1/2 transport. The potential of C. vulgaris to bio-remediate arsenic from water appeared to be highly selective and effective without the potential hazard of reducing As(V) to As(III), which is more toxic to humans.

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Identification and quantification of phytochelatins in roots of rice to long-term exposure: evidence of individual role on arsenic accumulation and translocation

Abstract: SummarySix varieties of rice were exposed to low and high levels of arsenic in the same soil. Their individual responses of expressing phytochelatins have been correlated to inorganic arsenic uptake, transport, and accumulation in the rice grain.

Cited by 155 publications

References 52 publications

Silicon mitigates oxidative stress and has positive effects in Eucalyptus platyphylla under aluminium toxicity

Silicon mitigates oxidative stress and has positive effects in Eucalyptus platyphylla under aluminium toxicity

The transportation and accumulation of arsenic, cadmium, and phosphorus in 12 wheat cultivars and their relationships with each other

The mechanisms of detoxification of As(III), dimethylarsinic acid (DMA) and As(V) in the microalga Chlorella vulgaris

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