Identification and quantification of valuable plant substances by IR and Raman spectroscopy

Schulz, Hartwig; Barańska, Małgorzata

doi:10.1016/j.vibspec.2006.06.001

Cited by 827 publications

(568 citation statements)

References 66 publications

(103 reference statements)

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“…FTIR spectroscopy measures the vibrations of bonds within functional groups and generates a spectrum that can be regarded as a metabolic fingerprint. Similar IR spectral profiles (3000 -600 cm -1 , figure 4) were found by a preliminary visual analysis for purpose of an exploratory overview of data, revealing typical signals of e.g., lipids (2910 -2845 cm -1 ), monoterpenes (1732, 1592, 1114, 1022, 972 cm -1 ), sesquiterpenes (1472 cm -1 ), and sucrose (1122 cm -1 - Schulz & Baranska, 2007) for all the studied samples. However, we were not able to identify by visual inspection of the spectra a clear picture regarding a discriminating effect of any primary or secondary metabolites among the propolis samples.…”

Section: Attenuated Total Reflectance-fourier Transform Infrared Specsupporting

confidence: 71%

“…Indeed, the development of Fourier transform (FT) IR and attenuated total reflectance (ATR) techniques have also evolved allowing rapid IR measurements of organosolvent extracts of plant tissues, edible oils, and essential oils, for example (Damm et al, 2005;Lai et al, 1994;Schulz & Baranska, 2007). In consequence of the strong dipole moment of water, IR spectroscopy applications have mostly focused on the analysis of dried or non-aqueous plant matrices and currently IR methods are widely used as a fast analytical technique for the authentication and detection of adulteration of vegetable oils.…”

Section: Attenuated Total Reflectance-fourier Transform Infrared Specmentioning

confidence: 99%

See 1 more Smart Citation

Metabolomics and Chemometrics as Tools for Chemo(bio)diversity Analysis - Maize Landraces and Propolis

Maraschin¹,

Kuhnen²,

Lemos³

et al. 2012

Chemometrics in Practical Applications

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Section: Attenuated Total Reflectance-fourier Transform Infrared Specsupporting

confidence: 71%

Section: Attenuated Total Reflectance-fourier Transform Infrared Specmentioning

confidence: 99%

Metabolomics and Chemometrics as Tools for Chemo(bio)diversity Analysis - Maize Landraces and Propolis

Maraschin¹,

Kuhnen²,

Lemos³

et al. 2012

Chemometrics in Practical Applications

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“…The two techniques, commonly referred to as vibrational or molecular spectroscopy, offer chemical characterization of plant samples via identifiable spectral features. A vibrational spectrum of a plant sample contains specific signatures of the constituent compounds, such as water, lipids, proteins, carbohydrates, pigments and complex biopolymers (Schulz and Baranska 2007). A major advantage of these techniques is that they can provide economical high-throughput analysis of biological samples without any chemical pretreatment.…”

Section: Introductionmentioning

confidence: 99%

Vibrational microspectroscopy enables chemical characterization of single pollen grains as well as comparative analysis of plant species based on pollen ultrastructure

Zimmermann

Bağcıoğlu

Sandt

et al. 2015

Planta

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Main conclusion: Chemical imaging of pollen by vibrational microspectroscopy enables characterization of pollen ultrastructure, in particular phenylpropanoid components in grain wall for comparative study of extant and extinct plant species.Keywords: FTIR microspectroscopy, Raman microspectroscopy, Pinales, imaging, cell wall. 2 SUMMARYA detailed characterization of conifer (Pinales) pollen by vibrational microspectroscopy is presented. The main problems that arise during vibrational measurements were scatter and saturation issues in Fourier transform infrared (FTIR), and fluorescence and penetration depth issues in Raman. Single pollen grains larger than approx. 15 µm can be measured by FTIR microspectroscopy using conventional light sources, while smaller grains may be measured by employing synchrotron light sources. Pollen grains that were larger than 50 µm were too thick for FTIR imaging since the grain constituents absorbed almost all infrared light. Chemical images of pollen were obtained on sectioned samples, unveiling the distribution and concentration of proteins, carbohydrates, sporopollenins and lipids within pollen substructures. The comparative analysis of pollen species revealed that, compared with other Pinales pollens, Cedrus atlantica has a higher relative amount of lipid nutrients, as well as different chemical composition of grain wall sporopollenin. The pre-processing and data analysis, namely extended multiplicative signal correction and principal component analysis, offer simple estimate of imaging spectral data and indirect estimation of physical properties of pollen. The vibrational microspectroscopy study demonstrates that detailed chemical characterization of pollen can be obtained by measurement of an individual grain and pollen ultrastructure. Measurement of phenylpropanoid components in pollen grain wall could be used, not only for the reconstruction of past environments, but for assessment of diversity of plant species as well. Therefore, analysis of extant and extinct pollen species by vibrational spectroscopies is suggested as a valuable tool in biology, ecology and palaeosciences.3

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“…44 The weak band at 3009 cm -1 can be attributed to C−H stretching in =C−H. 51 This indicates the presence of unsaturated fatty acids.…”

Section: Ftir Fingerprint Analysismentioning

confidence: 99%

Chemometric Analysis of UV Characteristic Profile and Infrared Fingerprint Variations ofCoffea arabicaGreen Beans under Different Space Management Treatments

Terrile¹,

Marcheafave²,

Oliveira³

et al. 2016

Journal of the Brazilian Chemical Society

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Ultraviolet characteristic profiles and infrared spectroscopic (FTIR) fingerprints of green bean extracts of Coffea arabica L., cv. IAPAR 59, cultivated in two planting patterns, rectangular and square, and at two different densities, 10,000 and 6,000 plants ha -1 , identified as R 10 , R 6 , S 10 , and S 6 were analyzed with principal component and hierarchical cluster analyses. A simplex centroid design for four solvents (ethanol, acetone, dichloromethane, hexane) was used for sample extraction. The largest chlorogenic acid (CGA) contents were found at the lower planting density. The dichloromethane extracts of the S 10 treatment showed the highest levels of unsaponifiable lipids (cafestol and kahweol). The R 6 treatment showed a slightly higher content of cafestol and kahweol. Cluster analysis of FTIR fingerprints confirmed that the CGA and caffeine levels differentiate the spatial arrangements. The FTIR fingerprints suggest that green beans from S 6 and R 10 were richer in lipids and the other two treatments had more sugars and proteins.

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Identification and quantification of valuable plant substances by IR and Raman spectroscopy

Cited by 827 publications

References 66 publications

Metabolomics and Chemometrics as Tools for Chemo(bio)diversity Analysis - Maize Landraces and Propolis

Metabolomics and Chemometrics as Tools for Chemo(bio)diversity Analysis - Maize Landraces and Propolis

Vibrational microspectroscopy enables chemical characterization of single pollen grains as well as comparative analysis of plant species based on pollen ultrastructure

Chemometric Analysis of UV Characteristic Profile and Infrared Fingerprint Variations ofCoffea arabicaGreen Beans under Different Space Management Treatments

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