Identification and Quantitation of Acetone Extractives of Wood and Bark by Ion Exchange and Capillary GC with a Spreadsheet Program

Sitholé, Bruce; Sullivan, Joseph; Allen, L. H.

doi:10.1515/hfsg.1992.46.5.409

Cited by 32 publications

(13 citation statements)

References 8 publications

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“…The capillary column used was a raphy (GC) and GC-mass spectrometry (MS) using high-temperature, polyimide-coated fused-silica tubshort-and medium-length high-temperature capillary ing DB5-HT (0.25 mm I.D., 0.1 mm film thickness) columns (with thin films), respectively, that enable from J&W Scientific (Folsom, CA, USA), specially elution and separation of high-molecular-mass lipids processed for an extended temperature of 4008C. [6][7][8][9][10][11], are employed.…”

Section: Problems Recent Advances In Biotechnology Havementioning

confidence: 99%

“…Several authors have used short GC conventional capillary columns, which allow the elution and separation of high-molecular-mass lipids, for the analysis of wood lipophilic extractives, although they do not allow the best resolution [10,11]. In the present study, the range of temperature of the analysis have been extended by using high-temperature capillary columns.…”

Section: Rapid Analysis Of Wood Lipophilic Extractives By Gc and Gc-msmentioning

confidence: 99%

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Analysis of lipophilic extractives from wood and pitch deposits by solid-phase extraction and gas chromatography

Gutiérrez

Rı́o

González-Vila

et al. 1998

Journal of Chromatography A

104

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An analytical procedure for the analysis of lipophilic extractives from wood and pitch deposits is described in this paper. It comprises a rapid gas chromatographic method that enables the analysis of a high number of samples in a short period of time. Short-length, high-temperature capillary columns with thin films and high temperature-programming rates were preferred for the rapid analysis of wood extractives since they enable elution and separation of compounds within a wide molecular mass range (from fatty acids to sterol esters and triglycerides) in the same chromatographic analysis in a short period of time. Several examples of analysis of extractives from pine and eucalypt woods and pitch deposits in an eucalypt kraft pulp, are shown. On the other hand, a simple fractionation method using solid-phase extraction (SPE) in aminopropyl cartridges is described for the preparative scale separation and fractionation of wood lipophilic extractives into major lipid classes. The SPE advantages include smaller sample and solvent requirements and ease of use compared to conventional solvent extraction techniques.

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Section: Problems Recent Advances In Biotechnology Havementioning

confidence: 99%

Section: Rapid Analysis Of Wood Lipophilic Extractives By Gc and Gc-msmentioning

confidence: 99%

Analysis of lipophilic extractives from wood and pitch deposits by solid-phase extraction and gas chromatography

Gutiérrez

Rı́o

González-Vila

et al. 1998

Journal of Chromatography A

104

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show abstract

“…However, sterols and some sterol esters and waxes, do not form soluble soaps under the alkaline conditions used in kraft pulping, and therefore, have a tendency to deposit and cause pitch problems (Swan 1967;Affleck and Ryan 1969;Leone and Breuil 1998). The higher concentration of these compounds in relation to the saponifiable extractives is the main cause for pitch problems in the kraft pulping of some hardwoods commonly used in the pulp and paper industry, such as aspen or eucalypt (Swan 1967;Allen 1988;Allen et al 1991;Dunlop-Jones et al 1991;Sitholé et al 1992;Chen et al 1995;Leone and Breuil 1998).…”

Section: Introductionmentioning

confidence: 99%

“…A solid-phase extrac-tion and a chromatographic procedure developed for the analysis of wood extractives (Gutiérrez et al 1998) were used in the present study. Short and medium-length high temperature capillary columns with thin films, which allow the elution and separation of high-molecular-mass lipids such as waxes, sterol esters and triglycerides (Wakeham and Frew 1982;Lusby et al 1984;Evershed et al 1989;Sitholé et al 1992;Örså and Holmbom 1994) were used for the gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analyses. This work is part of a wider project aimed at the evaluation of the viability of biotechnological solutions for eliminating problematic lipids from both wood and pulp by treatment with fungi and enzymes, respectively, thus introducing environmentally-friendly procedures in pulp and paper manufacture.…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition of Lipophilic Extractives from Eucalyptus globulus Labill. Wood

Gutiérrez¹,

Río²,

González-Vila³

et al. 1999

Holzforschung

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SummaryThe composition of lipophilic extractives in the chloroform soluble fraction of the acetone extract from Eucalyptus globulus wood has been examined. The lipid extract was fractionated by solid-phase extraction on aminopropyl-phase cartridges into four different fractions of increasing polarity. The total lipid extract and the resulting fractions were analyzed by gas chromatography and gas chromatography-mass spectrometry, using high temperature capillary columns. The main compounds identified included sterols, sterol esters, fatty acids, steroid ketones, hydrocarbons and triglycerides. Minor compounds such as fatty alcohols, mono-and diglycerides, waxes and tocopherols were also identified among the lipids from E. globulus wood.

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“…The injector was temperature programmed (GC-MS) using high-temperature short capillary from 1208C (0.1 min) to 3808C at a rate of 2008C / columns which have shown to be useful for the min and held until the end of the analysis. Helium analysis of wood extractives [5][6][7][8]. On the other was used as carrier gas.…”

Section: Introductionmentioning

confidence: 99%

Analysis of impurities occurring in a totally chlorine free-bleached Kraft pulp

Rı́o¹,

Gutiérrez²,

González-Vila³

1999

Journal of Chromatography A

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A set of impurities (specks) occurring in a TCF (totally chlorine free)-bleached Kraft pulp of Eucalyptus globulus wood was studied. The impurities were Soxhlet extracted with acetone, and the extracts subsequently analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) using high-temperature capillary columns. The lipophilic fraction isolated from E. globulus wood extractives was also analyzed for comparison. The composition of the acetone extracts was very similar to that of E. globulus wood. Fatty acids, steroid hydrocarbons, sterols, steroid ketones and sterol esters, arising from E. globulus wood extractives survived the cooking and bleaching processes and accumulated in the pulp. On the other hand, the residue left after acetone extraction was studied by pyrolysis-GC-MS. The results indicated that it was composed of small particles of polyisoprene rubber. In conclusion, the speck impurities studied here seems to be composed of two different moieties, a lipophilic part arising from wood extractives and a core of small particles of synthetic polymers (polyisoprene rubber). 

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Identification and Quantitation of Acetone Extractives of Wood and Bark by Ion Exchange and Capillary GC with a Spreadsheet Program

Cited by 32 publications

References 8 publications

Analysis of lipophilic extractives from wood and pitch deposits by solid-phase extraction and gas chromatography

Analysis of lipophilic extractives from wood and pitch deposits by solid-phase extraction and gas chromatography

Chemical Composition of Lipophilic Extractives from Eucalyptus globulus Labill. Wood

Analysis of impurities occurring in a totally chlorine free-bleached Kraft pulp

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