Identification and Quantitation of Superoxide Anion: Essential Steps in Elucidation of the Phagocyte “Respiratory Burst”

Nauseef, William M.

doi:10.4049/jimmunol.1402580

Cited by 10 publications

(5 citation statements)

References 34 publications

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“…Priming of the respiratory burst in neutrophils in suspension can be evaluated in vitro using the superoxide dismutase (SOD)‐inhibitable cytochrome c reduction assay, the standard technique that specifically measures superoxide production, but also by luminol‐amplified chemiluminescence or fluorescent probes‐based flow cytometry that measure ROS . SOD‐inhibitable cytochrome c reduction assay has the advantages to be specific for extracellular superoxide anions and to be a quantitative method . Luminol‐amplified chemiluminescence method is neither specific for superoxide anions nor quantitative for ROS, but has the advantages to be very sensitive and to detect both extracellular and intracellular ROS.…”

Section: Technical Considerations To Assess the Priming Of The Neutromentioning

confidence: 99%

Priming of the neutrophil respiratory burst: role in host defense and inflammation

El-Benna

Hurtado-Nédelec

Marzaioli

et al. 2016

Immunological Reviews

377

328

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Neutrophils are the major circulating white blood cells in humans. They play an essential role in host defense against pathogens. In healthy individuals, circulating neutrophils are in a dormant state with very low efficiency of capture and arrest on the quiescent endothelium. Upon infection and subsequent release of pro-inflammatory mediators, the vascular endothelium signals to circulating neutrophils to roll, adhere, and cross the endothelial barrier. Neutrophils migrate toward the infection site along a gradient of chemo-attractants, then recognize and engulf the pathogen. To kill this pathogen entrapped inside the vacuole, neutrophils produce and release high quantities of antibacterial peptides, proteases, and reactive oxygen species (ROS). The robust ROS production is also called 'the respiratory burst', and the NADPH oxidase or NOX2 is the enzyme responsible for the production of superoxide anion, leading to other ROS. In vitro, several soluble and particulate agonists induce neutrophil ROS production. This process can be enhanced by prior neutrophil treatment with 'priming' agents, which alone do not induce a respiratory burst. In this review, we will describe the priming process and discuss the beneficial role of controlled neutrophil priming in host defense and the detrimental effect of excessive neutrophil priming in inflammatory diseases.

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Section: Technical Considerations To Assess the Priming Of The Neutromentioning

confidence: 99%

Priming of the neutrophil respiratory burst: role in host defense and inflammation

El-Benna

Hurtado-Nédelec

Marzaioli

et al. 2016

Immunological Reviews

377

328

View full text Add to dashboard Cite

show abstract

“…n = 10. *P < 0.05versus apoptosis cell in LPS group; # P < 0.05 versus dead cell in LPS group advantages of being a quantitative method that is specific for extracellular superoxide anions [24]. Luminolamplified chemiluminescence method has the advantages of being very sensitive and able to detect both extracellular and intracellular ROS.…”

Section: Discussionmentioning

confidence: 95%

“…In this study, we evaluated the initiation of respiratory burst of suspended granulocytes in vitro using a SOD-inhibited cytochrome c reduction test, and luminol-amplified chemiluminescence to measure ROS [ 23 ]. SOD-inhibitable cytochrome c reduction assay is a standard technique for measuring superoxide dismutase production and has the advantages of being a quantitative method that is specific for extracellular superoxide anions [ 24 ]. Luminol-amplified chemiluminescence method has the advantages of being very sensitive and able to detect both extracellular and intracellular ROS.…”

Section: Discussionmentioning

confidence: 99%

Emodin alleviates LPS-induced inflammatory response in lung injury rat by affecting the function of granulocytes

et al. 2020

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Background: Acute lung injury (ALI) and/or acute respiratory distress syndrome (ARDS) are critical life-threatening syndromes characterized by the infiltration of a large number of granulocytes (mainly neutrophils) that lead to an excessive inflammatory response. Emodin (Emo) is a naturally occurring anthraquinone derivative and an active ingredient of Chinese medicine. It is believed to have anti-inflammatory effects. In this study, we examined the impact of Emo on the pulmonary inflammatory response and the granulocytes function in a rat model of lipopolysaccharide (LPS)-induced ALI. Results: Treatment with Emo protected rat against LPS-induced ALI. Compared to untreated rat, Emo-treated rat exhibited significantly ameliorated lung pathological changes and decreased tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). However, Emo has no protective effect on the rat model of acute lung injury with granulocyte deficiency. In addition, treatment with Emo enhanced the bactericidal capacity of LPS-induced granulocytes via the up-regulation of the ability of granulocytes to phagocytize bacteria and generate neutrophil extracellular traps (NETs). Emo also downregulated the respiratory burst and the expression of reactive oxygen species (ROS) in LPS-stimulated granulocytes, alleviating the damage of granulocytes to surrounding tissues. Finally, Emo can accelerate the resolution of inflammation by promoting apoptosis of granulocytes. Conclusion: Our results provide the evidence that Emo could ameliorates LPS-induced ALI via its anti-inflammatory action by modulating the function of granulocytes. Emo may be a promising preventive and therapeutic agent in the treatment of ALI.

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“…In this study, we evaluated the initiation of respiratory burst of suspended neutrophils in vitro using a SOD-inhibited cytochrome c reduction test, and luminol-amplified chemiluminescence to measure ROS [23] . SOD-inhibitable cytochrome c reduction assay is a standard technique for measuring superoxide dismutase production and has the advantages of being a quantitative method that is specific for extracellular superoxide anions [24] . Luminol-amplified chemiluminescence method has the advantages of being very sensitive and able to detect both extracellular and intracellular ROS.…”

Section: Discussionmentioning

confidence: 99%

Emodin alleviates LPS-induced inflammatory response in lung injury rat by affecting the function of neutrophils

Mei

Ying

Zhang

et al. 2020

Preprint

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Background: Acute lung injury (ALI) and/or acute respiratory distress syndrome (ARDS) are critical life-threatening syndromes characterized by the infiltration of a large number of neutrophils that lead to an excessive inflammatory response. Emodin (Emo) is a naturally occurring anthraquinone derivative and an active ingredient of Chinese medicine. It is believed to have anti-inflammatory effects. In this study, we examined the impact of Emo on the pulmonary inflammatory response and the neutrophil function in a rat model of lipopolysaccharide (LPS)-induced ALI. Results: Treatment with Emo protected rat against LPS-induced ALI. Compared to untreated rat, Emo-treated rat exhibited significantly ameliorated lung pathological changes and decreased tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). However, Emo has no protective effect on the rat model of acute lung injury with neutrophil deficiency. In addition, treatment with Emo enhanced the bactericidal capacity of LPS-induced neutrophils via the up-regulation of the ability of neutrophils to phagocytize bacteria and generate neutrophil extracellular traps (NETs). Emo also downregulated the neutrophil respiratory burst and the expression of reactive oxygen species (ROS) in LPS-stimulated neutrophils, alleviating the damage of neutrophils to surrounding tissues. Finally, Emo can accelerate the resolution of inflammation by promoting apoptosis of neutrophils. Conclusion:Our results provide the evidence that Emo could ameliorates LPS-induced ALI via its anti-inflammatory action by modulating the function of neutrophils. Emo may be a promising preventive and therapeutic agent in the treatment of ALI.

show abstract

Identification and Quantitation of Superoxide Anion: Essential Steps in Elucidation of the Phagocyte “Respiratory Burst”

Cited by 10 publications

References 34 publications

Priming of the neutrophil respiratory burst: role in host defense and inflammation

Priming of the neutrophil respiratory burst: role in host defense and inflammation

Emodin alleviates LPS-induced inflammatory response in lung injury rat by affecting the function of granulocytes

Emodin alleviates LPS-induced inflammatory response in lung injury rat by affecting the function of neutrophils

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