Identification and quantitative analysis of lipids and other organic compounds contained in eggs of Colorado potato beetle (Leptinotarsa decemlineata)

Abstract

The importance of insects in the environment is huge, not always positive. Harmful insects are burdensome and cause large economic losses. One of the representatives of harmful insects is potato beetle (Leptinotarsa decemlineata). Researching the chemical composition of this insect can be used to more effectively protect plants against this pest. The eggs of Colorado potato beetle (Leptinotarsa decemlineata) were chosen for analysis because their composition is little known. Samples for analysis were prepared using the Folch method, and gas chromatography coupled with mass spectrometry (GC/MS) was selected for final determinations. As a result of the analyses, carboxylic acids, dicarboxylic acids, hydroxy acids, monoacylglycerols, alcohols, sterols and other organic compounds were identified in the tested samples. The highest content was determined for acids with a carbon chain C16:0-C18:0: palmitic (23 ± 0.88 µg/g), linoleic (32 ± 2 µg/g), oleic (83 ± 8.7 µg/g) and stearic (50 ± 2.2 µg/g). In the case of alcohols and sterols, the highest content was determined for docosanol (2.5 ± 0.04 μg/g) and cholesterol (23 ± 1.4 µg/g), respectively. The knowledge of the chemical composition of eggs can help in the work on effective insecticides, which will be effective only on a species of harmful insect.

Virulence of entomopathogenic fungi against Culex pipiens: Impact on biomolecules availability and life table parameters

“…A serial dilution of oleic and palmitic acid mix. (7:3) was used to construct a standard curve (5–25 mg/ml) ( Wojciechowska et al, 2019 ).…”

“…Bovine Serum Albumin was used to construct a standard curve for the quantification of total protein in samples. The absorbance was measured at 595 nm ( Wojciechowska et al, 2019 ).…”

The composition of lipid profiles in different developmental stages of Dermestes ater and Dermestes maculatus and their susceptibility to the entomopathogenic fungus Conidiobolus coronatus

“…Typically, they contain 27 to 30 carbon atoms in the molecule. Cholesterol is the most commonly found steroid in insect lipids (Sreekantuswamy and Siddalingaiah 1981;Gołębiowski et al 2013;Cerkowniak et al 2013;Wojciechowska et al 2019). Cholesterol is present in all mammalian cells and body fluids.…”

The impact of insecticides containing deltamethrin and cyfluthrin on the composition of surface compounds in the larvae, females and males of Tenebrio molitor

“…Among the alkanes, mainly pentacosane, hexacosane, heptacosane and nonacosane were identified. The alkanes have also been described in many other insects: Lucilia sericata (Gołębiowski, Paszkiewicz, et al, 2012), Leptinotarsa decemlineata (Wojciechowska et al, 2019b), Anagasta kuehniella (Hebanowska et al, 1990), Blattella germanica (Augustynowicz et al, 1987), Aphthona lacertosa and Aphthona nigriscutis (Nelson et al, 2002).…”