Identification and relationships of accessions grown in the grapevine (Vitis vinifera L.) Germplasm Bank of Castilla y Léon (Spain) and the varieties authorized in the VQPRD areas of the region by SSR-marker analysis

Santana, Juan Carlos; Hidalgo, Elena; Lucas, Anne-Sophie; Recio, P.; Ortiz, Jesús; Martín, Juan José Molina; Yuste, J.; Arranz, C.; Rubio, José António

doi:10.1007/s10722-007-9261-2

Cited by 33 publications

(16 citation statements)

References 26 publications

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“…The Ho values of the VVMD24, VMC2C3, VVMD28, VVS2, VVIBO1 and VVMD31 loci were high. Previous reports also found relatively high Hes in some other grape loci (Costantini et al 2005, Santana et al 2007. In this study, the frequency of null alleles (r) at these VrZAG79, VVMD27, VVMD5, VrZAG62, VMC2H4, VVMD7, VVIH54 and VrZAG83 loci was positive, but these low values suggest the absence of null alleles ( Table 2).…”

Section: Ssr Analysissupporting

confidence: 55%

Genetic analysis of grapevine cultivars from the Eastern Mediterranean Region of Turkey, based on SSR markers

Gök;SOYDAM¹

2009

Tarım Bilimleri Dergisi

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: Turkey is one of the centres for grapevine diversity with many local grapevine cultivars with potentially desirable characteristics are still widely cultivated throughout several regions in the country. One of such regions is the Eastern Mediterranean region of the country. To characterize the grapevine germplasm of this region, we performed a genetic analysis of 59 grapevine cultivars using 14 simple sequence repeat (SSR) loci. The genetic relationships of the cultivars which did not appear to be linked to their ecogeographical distributions within the region were determined. Among the cultivars analyzed in this study, several synonymous and homonymous cultivars were identified, suggesting that some of the grapevine cultivars with different names might be genetically identical while some cultivars with the same names might be genetically different. This study represents a first step toward the genetic characterization of the grapevine genotypes of this region and will help promote more effective germplasm management and breeding programs.

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Section: Ssr Analysissupporting

confidence: 55%

Genetic analysis of grapevine cultivars from the Eastern Mediterranean Region of Turkey, based on SSR markers

Gök;SOYDAM¹

2009

Tarım Bilimleri Dergisi

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“…The experiments were carried out at the tissue culture and in vitro conservation laboratory of Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil, between 2007 and2008. Approximately 1.0-cm long microshoots, with one axillary bud and one lateral leaf, were taken from a total of ten grape genotypes: six V. vinifera genotypes ('Brasil', 'Flora', 'Sun Red', 'Emperor', 'Pinotage', and 'Zinfandel'), two V. labrusca genotypes ('Videira 889' and 'Goethe'), and two hybrids ('Baco Blanc' and 'Paulistinha').…”

Section: Methodsmentioning

confidence: 99%

“…The genetic diversity of perennial plants, including grapevines, is usually sampled and maintained as live plants in field gene banks (Santana et al, 2008;Leão & Motoike, 2011). These collections are constantly in danger of being lost by exposure to environmental adversity, pest attacks, propagation issues, and frequent budget constraints, not to mention their potential to spread pests and diseases, which are important barriers to germplasm exchange (Engelmann, 2009).…”

Section: Introductionmentioning

confidence: 99%

Short-term storage in vitro and large-scale propagation of grapevine genotypes

Silva

Luis

Scherwinski‐Pereira

2012

Pesq. agropec. bras.

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-The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, the best results for number of roots were obtained using a culture medium supplemented with 0.4 µmol L -1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20 o C, can be micropropagated in large scale.

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“…The values of the Ho were always higher than those of the He. This fact can be explained, according to Lopes et al (1999), by the artificial selection in order to improve the yields and quality; or perhaps, as suggested in Santana et al (2008), by the intention to maintain variability for satisfying preferences of the consumers in different regions.…”

Section: Discussionmentioning

confidence: 99%

Ampelography and microsatellite DNA analysis of autochthonous and endangered grapevine cultivars in the province of Huesca (Spain)

Casanova

Mozas

Ortiz

1970

Span. j. agric. res.

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The province of Huesca (Spain) is a traditional area for growing grapevines. There are archaeological evidences of their cultivation at least since the second century B.C. Nevertheless, in the last years the varietal diversity is markedly decreasing due to the rooting out of old plantations that are replaced with new broadly known varieties, since the market demands a certain standardization of the wine producing varieties. The objective of this study is to examine and characterise the existing varieties in the province of Huesca, mainly in vineyards planted before 1960. A total of 47 accessions were collected in 14 plots from 11 municipalities. Moreover 36 accessions from the region, planted at the Movera grapevine germplasm bank (Movera collection), located at Zaragoza (Spain) were included in the study. Ampelographic characterisation was carried out with 50 OIV descriptors for two consecutive years. Molecular characterisation was made with the following microsatellites: VVS2, VVMD5, VVMD7, VVMD27, VrZAG62 and VrZAG79. Ampelographic results were compared by cluster analysis. Most of the accessions were identified, being considered as new varieties: two white, Carrillera and Moscatel, and six red or black: Macicillo, Bomogastro, Angelina, Terrer, Parraleta Roja and Garnacha Gorda. Several new synonymies were detected, as Miguel for Vitadillo or Parrel for Trepat. Seven accessions remained unidentified. Out of the studied Vitis vinifera varieties, 23 were considered as endangered, indicating the valuable patrimony of the zone to be preserved.Additional key words: homonymy; minor varieties; synonymies; varietal identification; Vitis germplasm bank. ResumenAmpelografía y análisis de ADN con microsatélites de cultivares de vid autóctonos y en peligro de extinción en la provincia de Huesca (España)La provincia de Huesca (España) ha sido un lugar tradicional de cultivo de vid. Existe evidencia arqueológica de la existencia de dicho cultivo desde el siglo II a.C. Sin embargo, en las últimas décadas la diversidad varietal está disminuyendo marcadamente debido al arranque de antiguas plantaciones que están siendo reemplazadas con nuevas variedades ampliamente conocidas, ya que el mercado demanda una cierta estandarización de las variedades productoras de vino. El objetivo de este estudio es caracterizar e identificar las variedades existentes en la provincia de Huesca, principalmente en viñedos plantados antes de 1960. Se muestrearon 47 accesiones de 14 parcelas en 11 municipios. Además se incluyeron otras 36 accesiones de la provincia de Huesca que se encuentran en el banco de germoplasma de vid de Movera (colección de Movera), situado en Zaragoza (España). La caracterización ampelográfica se llevó a cabo con 50 descriptores durante dos años consecutivos. La caracterización molecular se realizó con los siguientes microsatélites: VVS2, VVMD5, VVMD7, VVMD27, VrZAG62 y VrZAG79. Los resultados ampelográficos se compararon mediante análisis de enjambres. Se identificó la mayor parte de las accesiones, detectándose l...

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Identification and relationships of accessions grown in the grapevine (Vitis vinifera L.) Germplasm Bank of Castilla y Léon (Spain) and the varieties authorized in the VQPRD areas of the region by SSR-marker analysis

Cited by 33 publications

References 26 publications

Genetic analysis of grapevine cultivars from the Eastern Mediterranean Region of Turkey, based on SSR markers

Genetic analysis of grapevine cultivars from the Eastern Mediterranean Region of Turkey, based on SSR markers

Short-term storage in vitro and large-scale propagation of grapevine genotypes

Ampelography and microsatellite DNA analysis of autochthonous and endangered grapevine cultivars in the province of Huesca (Spain)

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