Identification and structural characterization of lipid A from <scp>Escherichia coli</scp>, Pseudomonas putida and Pseudomonas taiwanensis using liquid chromatography coupled to high‐resolution tandem mass spectrometry

Froning, Matti; Helmer, Patrick O.; Hayen, Heiko

doi:10.1002/rcm.8897

Cited by 11 publications

(16 citation statements)

References 43 publications

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“…LPS is composed of lipid A, an oligosaccharide core and an O-antigen. The lipid A or O-antigen structure differs between the different species of Gram-negative bacteria [ 120 , 121 , 122 ]. On the other hand, Gram-positive bacteria contain glycolipids, glucolipids and lipoteichoic acid (LTA), which is a typical component of the cell membrane.…”

Section: Bacteria Identificationmentioning

confidence: 99%

“Omic” Approaches to Bacteria and Antibiotic Resistance Identification

Janiszewska

Szultka‐Młyńska

Pomastowski

et al. 2022

IJMS

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The quick and accurate identification of microorganisms and the study of resistance to antibiotics is crucial in the economic and industrial fields along with medicine. One of the fastest-growing identification methods is the spectrometric approach consisting in the matrix-assisted laser ionization/desorption using a time-of-flight analyzer (MALDI-TOF MS), which has many advantages over conventional methods for the determination of microorganisms presented. Thanks to the use of a multiomic approach in the MALDI-TOF MS analysis, it is possible to obtain a broad spectrum of data allowing the identification of microorganisms, understanding their interactions and the analysis of antibiotic resistance mechanisms. In addition, the literature data indicate the possibility of a significant reduction in the time of the sample preparation and analysis time, which will enable a faster initiation of the treatment of patients. However, it is still necessary to improve the process of identifying and supplementing the existing databases along with creating new ones. This review summarizes the use of “-omics” approaches in the MALDI TOF MS analysis, including in bacterial identification and antibiotic resistance mechanisms analysis.

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Section: Bacteria Identificationmentioning

confidence: 99%

“Omic” Approaches to Bacteria and Antibiotic Resistance Identification

Janiszewska

Szultka‐Młyńska

Pomastowski

et al. 2022

IJMS

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“…Another popular group in γ-Proteobacteria is the family Pseudomonadaceae . Pseudomonas aeruginosa and taxonomically related bacteria contain lipid A with fatty acids of shorter carbon chains [ 22 , 23 ]. The 2 and 2′ positions of the backbone are substituted by 3-hydroxylauric acid (3-OH-C 12:0 ), whereas the 3 and 3′ positions are substituted (but not fully) by 3-hydroxycapric acid (3-OH-C 10:0 ).…”

Section: Diversity Of Lipid a Structure In Various Taxonomic Groupmentioning

confidence: 99%

Variation, Modification and Engineering of Lipid A in Endotoxin of Gram-Negative Bacteria

Kawahara

2021

IJMS

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Lipid A of Gram-negative bacteria is known to represent a central role for the immunological activity of endotoxin. Chemical structure and biosynthetic pathways as well as specific receptors on phagocytic cells had been clarified by the beginning of the 21st century. Although the lipid A of enterobacteria including Escherichia coli share a common structure, other Gram-negative bacteria belonging to various classes of the phylum Proteobacteria and other taxonomical groups show wide variety of lipid A structure with relatively decreased endotoxic activity compared to that of E. coli. The structural diversity is produced from the difference of chain length of 3-hydroxy fatty acids and non-hydroxy fatty acids linked to their hydroxyl groups. In some bacteria, glucosamine in the backbone is substituted by another amino sugar, or phosphate groups bound to the backbone are modified. The variation of structure is also introduced by the enzymes that can modify electrostatic charges or acylation profiles of lipid A during or after its synthesis. Furthermore, lipid A structure can be artificially modified or engineered by the disruption and introduction of biosynthetic genes especially those of acyltransferases. These technologies may produce novel vaccine adjuvants or antagonistic drugs derived from endotoxin in the future.

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“…11,12 (A summary of important previous studies is given in Table S1.) In recent years, high-performance liquid chromatography (HPLC) has been increasingly utilized, [13][14][15][16] which has significantly improved the separation efficiency and reduced the running time for PPCPs separations. 17 The concentrations of most PPCPs in environmental samples range from ng/L to μg/L, which requires solid-phase extraction (SPE) to enrich the PPCPs before analysis.…”

Section: Introductionmentioning

confidence: 99%

An advanced LC–MS/MS protocol for simultaneous detection of pharmaceuticals and personal care products in the environment

Yao

et al. 2022

Rapid Comm Mass Spectrometry

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Rationale The development of appropriate analytical screening techniques for pharmaceuticals and personal care products (PPCPs) is the basis for studying the distribution and environmental impact of emerging contaminants (ECs). Mass spectrometry‐based screening methods vary with the complexity of the target compounds. It is challenging to balance both positive and negative ion quantification with a low detection limit. To establish a set of experimental methods including extraction, chromatography‐separation and mass spectrometry screening is one of the most important topics in PPCP research. This paper describes a universal and efficient qualification and quantification protocol for the simultaneous detection of 34 PPCPs in different environmental samples in a single analytical data acquisition run. Methods Thirty‐four representative PPCPs, which are widely distributed in the environment with high ecological toxicity and complex chemical structures, were selected as representative target ECs. The extraction of the target PPCPs was achieved using only one solid‐phase extraction cartridge without the need to adjust the pH of samples. The enriched samples were detected by LC–MS/MS in both positive and negative ion modes simultaneously. The protocol was evaluated based on the accuracy, precision, detection limits and matrix effects. Results This method achieved simultaneous detection of PPCPs in both positive and negative ion modes, with a single analytical cycle of 12 min. The observed SPE recoveries were between 40% and 115%. The instrumental detection limits (IDL) varied from 0.01 to 1 pg, and the method detection limits (MDL) were between 0.002 and 3.323 ng/l in different matrices. Most of the PPCPs were subjected to matrix suppression below 30%. The method was successfully applied for quantitative analysis of the PPCPs in different environmental samples, including river samples, wastewater treatment plant (WWTP) samples and soil samples. Conclusions This protocol developed a rapid and efficient detection method to simultaneous qualitative and quantitative 34 representative PPCPs in the environment. The IDL ranged from 0.01 to 1 pg and the MDL ranged from 0.002 to 3.323 ng/l in different matrices. The detection limit was one order of magnitude lower compared to previous studies. The protocol also provided a wide application range for different environmental matrices, which permitted the migration and transformation of PPCPs to be explored.

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Identification and structural characterization of lipid A from Escherichia coli, Pseudomonas putida and Pseudomonas taiwanensis using liquid chromatography coupled to high‐resolution tandem mass spectrometry

Cited by 11 publications

References 43 publications

“Omic” Approaches to Bacteria and Antibiotic Resistance Identification

“Omic” Approaches to Bacteria and Antibiotic Resistance Identification

Variation, Modification and Engineering of Lipid A in Endotoxin of Gram-Negative Bacteria

An advanced LC–MS/MS protocol for simultaneous detection of pharmaceuticals and personal care products in the environment

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