Identification and Structure Elucidation of a Novel Antifungal Compound Produced by Pseudomonas aeruginosa PGPR2 Against Macrophomina phaseolina

Illakkiam, Devaraj; Ponraj, Paramasivan; Shankar, Manoharan; Muthusubramanian, Shanmugam; Rajendhran, Jeyaprakash; Gunasekaran, Paramasamy

doi:10.1007/s12010-013-0469-7

Cited by 20 publications

(12 citation statements)

References 30 publications

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“…These P. aeruginosa products include phenazines such as pyocyanin (5-N-methyl-1-hydroxyphenazine) (19)(20)(21)(22), 1-hydroxyphenazine (19,21,22), phenazine-1-carboxamide, phenazine-1-carboxylic acid (22), and dirhamnolipids (23). Other P. aeruginosa products have been noted to be toxic to other fungi, though their effects on A. fumigatus have not been studied, including 3,4-dihydroxy-2-heptylquinoline (PQS) and phenazin-1-ol (24), 3,4-dihydroxy-N-methyl-4-(4-oxochroman-2-yl)butanamide (25), and lipopolysaccharide (26). However, studying individual molecules for toxicity does not give insight into their relative contributions in a mixture, how the molecules would behave in the presence of other P. aeruginosa and/or A. fumigatus metabolites, or whether the concentrations studied are relevant to those that occur in intermicrobial interactions (27,28).…”

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confidence: 99%

Studies of Pseudomonas aeruginosa Mutants Indicate Pyoverdine as the Central Factor in Inhibition of Aspergillus fumigatus Biofilm

et al. 2018

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and are common opportunistic bacterial and fungal pathogens, respectively. They often coexist in airways of immunocompromised patients and individuals with cystic fibrosis, where they form biofilms and cause acute and chronic illnesses. Hence, the interactions between them have long been of interest and it is known that can inhibit We have approached the definition of the inhibitory molecules by studying 24 mutants with various virulence genes deleted for the ability to inhibit biofilms. The ability of cells or their extracellular products produced during planktonic or biofilm growth to affect biofilm metabolism or planktonic growth was studied in agar and liquid assays using conidia or hyphae. Four mutants, the, ,, and mutants, were shown to be defective in various assays. This suggested the siderophore pyoverdine as the key inhibitory molecule, although additional quorum sensing-regulated factors likely contribute to the deficiency of the latter two mutants. Studies of pure pyoverdine substantiated these conclusions and included the restoration of inhibition by the pyoverdine deletion mutants. A correlation between the concentration of pyoverdine produced and antifungal activity was also observed in clinical isolates derived from lungs of cystic fibrosis patients. The key inhibitory mechanism of pyoverdine was chelation of iron and denial of iron to Interactions between human pathogens found in the same body locale are of vast interest. These interactions could result in exacerbation or amelioration of diseases. The bacterium affects the growth of the fungus Both pathogens form biofilms that are resistant to therapeutic drugs and host immunity. and biofilms are found , e.g., in the lungs of cystic fibrosis patients. Studying 24 mutants, we identified pyoverdine as the major anti- compound produced by Pyoverdine captures iron from the environment, thus depriving of a nutrient essential for its growth and metabolism. We show how microbes of different kingdoms compete for essential resources. Iron deprivation could be a therapeutic approach to the control of pathogen growth.

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Studies of Pseudomonas aeruginosa Mutants Indicate Pyoverdine as the Central Factor in Inhibition of Aspergillus fumigatus Biofilm

et al. 2018

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“…Lytic enzymes, such as chitinase, proteases, or cellulases produced by antagonistic microorganisms, are responsible for the lysis or hyperparasitism expressed against deleterious fungal pathogens or insects. In the biological control mechanisms, these extracellular enzymes released by beneficial microorganisms are digesting the cell wall components of the phytopathogenic fungi (Illakkiam et al, 2013). Such enzymatic activity was found in all Bacillus strains analyzed in this study (Table 2), suggesting these beneficial properties as additional mechanism in their biological control activity.…”

Section: Bacillus Enzymatic Characterizationmentioning

confidence: 60%

Enzimatic and Genetic Variability in Bacillus spp. Strains with Plant Beneficial Qualities

et al. 2016

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Plant health is one of the issues that have to be maintained and closely monitored during cultivation and harvest. In this regard, prevention is the key factor in organic production. Biological control of plant pathogens and plant growth stimulation can be done through beneficial microorganisms. Different bacterial bio-preparates are available on the market, many of them based on selected strains of Bacillus species. In our previous studies, we isolated autochthones strains of Bacillus spp. with beneficial traits for plant protection and growth promotion. Considering the interest for biological production, and public concern for healthy products without significantly reduced yield, different biological control strains of Bacillus spp. that could be used as bio-inoculants for plant protection were analyzed.Thirteen biocontrol strains of Bacillus spp. were analyzed in comparison with three referent strains of B.subtilis and B.licheniformis. Qualitative and semi-quantitative analysis of these biocontrol Bacillus spp. strains was studied in order to characterize their enzymatic activity with implications either in cell wall degradation of plant pathogenic microorganisms, or in metabolism of various substrates. Genetic variability was studied by rep-PCR analysis compared with reference strains of B.subtilis and B.licheniformis. Microbiological studies performed in order to characterize the selected beneficial bacteria for their ability to produce lytic enzyme involved in plant pathogenic inhibition and plant growth stimulation revealed chitinase, cellulose, protease, lipase, amylase, decarboxylase, ACC-deaminase and phosphatease activity. The molecular techniques revealed significant genetic differences among the bacterial strains analyzed. The study allowed the detection of several enzymatic mechanisms involved in plant growth and protection, and revealed the potential of autochthon microbiota to be used for biotechnological purposes.

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“…This strain also had a strong suppressive effect on the growth of Macrophomina phaseolina . Earlier, we have reported a secondary metabolite (3, 4-dihydroxy-N-methyl-4-(4-oxochroman-2-yl) butanamide) and a secreted protease responsible for the antifungal activity [7, 25]. Among several reported strains, P. aeruginosa M18 strain was the only reported genome with plant growth promotion and biocontrol activity [26].…”

Section: Resultsmentioning

confidence: 99%

“…These metabolites include phenazine, pyocyanin, paerucumarin, pyoverdine, pyochelin, hydrogen cyanide, AHLs, rhamnolipids, phenylacetic acid, and macrolide antibiotics. In addition, we have demonstrated earlier that 3, 4-dihydroxy-N-methyl-4-(4-oxochroman-2-yl) butanamide, a secondary metabolite synthesized by PGPR2, showed antagonism against Macrophomina phaseolina [7]. Based on the survey of the PGPR2 genome putative biosynthetic pathway was identified.…”

Section: Resultsmentioning

confidence: 99%

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Genome Sequencing of a Mung Bean Plant Growth Promoting Strain ofP. aeruginosawith Biocontrol Ability

Illakkiam

Shankar

Ponraj

et al. 2014

International Journal of Genomics

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Pseudomonas aeruginosa PGPR2 is a mung bean rhizosphere strain that produces secondary metabolites and hydrolytic enzymes contributing to excellent antifungal activity against Macrophomina phaseolina, one of the prevalent fungal pathogens of mung bean. Genome sequencing was performed using the Ion Torrent Personal Genome Machine generating 1,354,732 reads (6,772,433 sequenced bases) achieving ~25-fold coverage of the genome. Reference genome assembly using MIRA 3.4.0 yielded 198 contigs. The draft genome of PGPR2 encoded 6803 open reading frames, of which 5314 were genes with predicted functions, 1489 were genes of known functions, and 80 were RNA-coding genes. Strain specific and core genes of P. aeruginosa PGPR2 that are relevant to rhizospheric habitat were identified by pangenome analysis. Genes involved in plant growth promoting function such as synthesis of ACC deaminase, indole-3-acetic acid, trehalose, mineral scavenging siderophores, hydrogen cyanide, chitinases, acyl homoserine lactones, acetoin, 2,3-butanediol, and phytases were identified. In addition, niche-specific genes such as phosphate solubilising 3-phytase, adhesins, pathway-specific transcriptional regulators, a diguanylate cyclase involved in cellulose synthesis, a receptor for ferrienterochelin, a DEAD/DEAH-box helicase involved in stress tolerance, chemotaxis/motility determinants, an HtpX protease, and enzymes involved in the production of a chromanone derivative with potent antifungal activity were identified.

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Identification and Structure Elucidation of a Novel Antifungal Compound Produced by Pseudomonas aeruginosa PGPR2 Against Macrophomina phaseolina

Cited by 20 publications

References 30 publications

Studies of Pseudomonas aeruginosa Mutants Indicate Pyoverdine as the Central Factor in Inhibition of Aspergillus fumigatus Biofilm

Studies of Pseudomonas aeruginosa Mutants Indicate Pyoverdine as the Central Factor in Inhibition of Aspergillus fumigatus Biofilm

Enzimatic and Genetic Variability in Bacillus spp. Strains with Plant Beneficial Qualities

Genome Sequencing of a Mung Bean Plant Growth Promoting Strain ofP. aeruginosawith Biocontrol Ability

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